RNA (Ribonucleic Acid) sequencing quality control method and device relating to gene expression

Abstract

The invention discloses RNA (Ribonucleic Acid) sequencing quality control method and device relating to gene expression. The method comprises the following steps of: respectively carrying out DGE (Digital Gene Expression) analysis and RNA-Seq (Ribonucleic Acid-Sequence) analysis on a sequencing segment obtained through a sequencing technology; respectively carrying out correlation analysis between a DGE analysis result and a qPCR (quantitative Polymerase Chain Reaction) result and between a transcriptome analysis result and the qPCR result; judging the difference of DGE analysis and the transcriptome analysis in gene expression quantification according to a correlation analysis result and selecting a sequencing analysis mode from the DGE analysis and the transcriptome analysis; and selecting 1M of reads from analysis results acquired from the selected sequencing analysis mode to analyze the sequencing stability of the gene expression. By carrying out the correlation analysis and the comprehensive estimation of the gene segment, the invention selects a gene expression analysis method with higher reliability, ensures the accuracy of the sequencing work and provides a quality control scheme for the production stability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a ribonucleic acid (RNA, RiboNucleic Acid) sequencing quality control method and device for gene expression. Background technique [0002] Gene expression refers to the process of transcribing gene fragment deoxyribonucleic acid (DNA, Deoxyribonucleic acid) into messenger ribonucleic acid (mRNA, Messenger RNA) and mRNA translation into protein. With the completion of all nucleotide sequencing of the Human Genome Project (HGP, Human Genome Project), the focus of human genome research has gradually entered the post-genome era (Postgenome Era) and tilted towards gene function and gene diversity. Through the parallel analysis of the expression of a large number of genes in individuals at different growth and development stages or in different physiological states, the functions of corresponding genes in organisms are studied, and the mechanism of multi-gene synergy at different levels is...

AI Technical Summary

Problems solved by technology

With the emergence of Solexa sequencing technology, high-throughput, low-cost sequencing has become possible, and compared with the analog signal of chip technology, expression analysis based on Solexa sequencing technology avoids cross-hybridization, complex analysis models and sensitivity in chip technology Low disadvantages; however, due to the limitation of high-throughput sequencing read length, its application in de novo sequencing of unknown genomes is limited, and this part of the work still requires traditional sequencing methods (read length can reach 850 base) assistance

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