Method for assisting identifying chicken with different fat traits and primer special for same

Abstract

The invention discloses a method for identifying chicken with different fat traits in an assisted manner and a primer special for the same. The method is characterized by detecting whether the nucleotide 301, from 5' terminal, of DNA shown in the sequence 3 in a sequence table in chromosome 1 of the chicken to be detected is T or C and determining whether the genetype of the chicken to be detected is TT, CT or CC, wherein the fat traits of the chicken with CC genetype are superior to the chicken with TT genetype and the chicken with CT genetype; the CC genetype is a homozygote with nucleotideat the site being C; the TT genetype is a homozygote with nucleotide at the site being T; and the CT genetype is a heterozygote of the CC genetype and the TT genetype. The method can assist screeningthe chicken with excellent fat traits (lower content of belly fat and simultaneously better growth conditions), has the advantages of quickness, simpleness and convenience and capability of screeningat early stage, has great significance in breeding the chicken and further has great significance in studying the mechanism of fat metabolism of the chicken.

Description

technical field [0001] The invention relates to a method for assisting in identifying chickens with different fat traits and special primers thereof. Background technique [0002] In 1990, Issemann et al. first discovered that PPAR, a new steroid hormone receptor, can be activated by a class of fatty acid-like compounds peroxisome proliferators (PP), so it was named peroxisome proliferator activation. receptor (PPAR). (Issemann I. and Green S. Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators. Nature, 1990, 347(6294): 645-50). PPARs belong to the nuclear receptor superfamily. There are currently three subtypes of PPARs: PPARα, PPARβ, and PPARγ. Nuclear receptors belong to fat-soluble hormone receptors, and their primary structures have high homology and contain the same functional domains, namely N-terminus (A / B region), DNA binding region (C region), connecting region ( D) and the ligand-binding region (E / F) at the C-terminus...

AI Technical Summary

Problems solved by technology

Determination is time-consuming and requires a large capital investment, making it difficult to apply to breeding practice

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