Application of in-situ crosslinking hydrogel capable of intraocular injection in preparing artificial vitreous bodies

An in-situ cross-linking and hydrogel technology, applied in prosthesis, medical science, etc., can solve the problem of lack of intraocular fillings and achieve the effect of no obvious toxic reaction

Inactive Publication Date: 2011-01-05
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, so far, there is a lack of ideal intraocular fillers clinically.

Method used

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  • Application of in-situ crosslinking hydrogel capable of intraocular injection in preparing artificial vitreous bodies
  • Application of in-situ crosslinking hydrogel capable of intraocular injection in preparing artificial vitreous bodies
  • Application of in-situ crosslinking hydrogel capable of intraocular injection in preparing artificial vitreous bodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, the cell safety test of the in situ cross-linked gel of the present invention

[0029] The cell experiment steps are as follows:

[0030] 1. Dissolve 4ARM-PEG-SH and PEG-S-EMA in phosphate buffer solution with pH = 7.4 and concentration of 0.1mol / L respectively, and make solutions with a concentration of 150mg / mL, and mix the two solutions with The volume ratio was 2:1 and mixed in vitro for 3 hours to obtain gel blocks for cell experiments.

[0031] 2. Cell lines (rat retinal pigment epithelial cell D407 cell line; monkey eye retinal vascular endothelial cell RF / 6A) (CRL-1780 cell line) were routinely cultured, and the culture medium was DMEM / F12=1:1 (purchased from Hyclone, USA company), add fetal bovine serum (final concentration is 10%), and get the cells passed to 3-6 passages for experiment.

[0032] 3. Digest the cells with 0.25% trypsin, mix well to make a cell suspension, count to 50000 / ml, take a sterile 96-well plate, add 100 μl of cell suspensio...

Embodiment 2

[0040] Example 2. Safety experiment of in situ cross-linked gel filling in animal eyes of the present invention

[0041] Five Qingzilan rabbits were selected for the experiment.

[0042] The animal experiment steps are as follows:

[0043] 1. Anesthetize the Qingzilan rabbit.

[0044] 2. Cut the hemibulbar conjunctiva along the limbus, and make perfusion holes and vitrectomy holes with a 19G scleral puncture knife.

[0045] 3. Insert the perfusion head and turn on the perfusion. The formula of the perfusion solution is as follows: add 50% glucose 4ml, dexamethasone (5mg / 1ml) 1.6ml, epinephrine hydrochloride (1mg / 1ml) 0.5ml to 500ml lactated Ringer’s solution, Tobramycin (80,000 U / 1ml) 0.2ml.

[0046] 4. The vitreous cutting head extends into the vitreous cavity to remove all the vitreous.

[0047] 5. Dissolve 4ARM-PEG-SH and PEG-S-EMA respectively in phosphate buffer solution with a concentration of 0.1mol / L at pH=7.4, and prepare a solution with a concentration of 150mg / m...

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PUM

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Abstract

The invention discloses an application of in-situ crosslinking hydrogel in preparing artificial vitreous bodies, which is characterized in that the in-situ crosslinking hydrogel is prepared by in-situ crosslinking after mixing a four-arm-end mercapto polyethylene glycol solution shown in the formula (I) and a polymer solution shown in the formula (II); wherein m in the formula (I) is an integer larger than 1, and n in the formula (II) is an integer larger than 1; and the two solutions are filled in eyes without vitreous bodies after mixing and can effectively form gel. A cell experiment and an animal in vivo experiment prove that the in-situ crosslinking hydrogel does not cause obvious inflammatory reaction and intraocular organ toxic reaction and can be used as the artificial vitreous bodies.

Description

technical field [0001] The invention relates to the application of an in-situ cross-linked hydrogel capable of intraocular injection in the preparation of artificial vitreous body. Background technique [0002] Vitrectomy is a commonly used clinical surgical method for the treatment of retinal detachment, vitreous hemorrhage, trauma to the posterior segment of the eye and other fundus diseases. After the vitreous body is removed, intraocular fillers need to be injected to reset the pressure on the retina and maintain the shape of the eyeball. Commonly used intraocular fillers include silicone oil and inert gas (such as SF 6 、C 2 f 6 and C 3 f 8 Wait). However, silicone oil filling has complications such as silicone oil emulsification, secondary glaucoma, corneal band degeneration, and concurrent cataract, which must be removed by a second operation. There is nothing that can be done for those whose eyes are dependent on silicone oil and the eyeballs shrink after the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/52C08J3/24C08J3/075C08L51/08C08L71/00
Inventor 陶勇姜燕荣郭宝华黄延宾童新明
Owner PEOPLES HOSPITAL PEKING UNIV
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