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Method for propagating potamogeton malaianus

A technology of marigolds and explants, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of reducing the degree of eutrophication of water bodies, unable to grow roots, unable to survive, etc. The effect of rooting, improving the survival rate, and meeting the needs of the project

Active Publication Date: 2011-12-07
南京中科水治理股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Restoring the population of sargassum can effectively reduce the degree of eutrophication in water bodies and increase the diversity of aquatic ecosystems. Therefore, sargassum is a conventional plant species planted in aquatic vegetation restoration and water eutrophication control projects, but There are certain difficulties in propagating S. japonica by cuttings. Direct cuttings of explants cannot grow roots for a long time and cannot survive.

Method used

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  • Method for propagating potamogeton malaianus
  • Method for propagating potamogeton malaianus

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1: the propagation method of a kind of kohlrabi of the present invention, comprises the steps:

[0018] 1. Collect well-grown and free of diseases and insect pests, and wash off the surface attachments of the cantaloupe with clean water;

[0019] 2. Cut the stems of the kohlrabi, keep 2 to 3 internodes for each section, 12 cm long, and remove the leaves;

[0020] 3. Cultivation conditions of explants: the sheared kohlrabi was cultured in nutrient solution containing phytohormone Hoagland, the temperature was kept at 25°C, the light intensity was 4000-5000LUX, and the day ratio was 1 : 1, after 10 days of cultivation, new roots and shoots will grow;

[0021] In addition to the basic elements of Hoagland's nutrient solution, each liter of Hoagland's nutrient solution also contains 1 mg of gibberellin and 1 mg of indole butyric acid;

[0022] To prepare 1 liter of Hoagland's nutrient solution:

[0023] (1) Preparation of iron salt solution

[0024] The formu...

Embodiment 2

[0039] Embodiment 2: a kind of method for propagating of Selina oleracea, its specific implementation steps are as follows:

[0040] 1. The well-grown and free-of-all diseases and insects of the canola collected in the river can be washed with clean water for 3 times to remove the surface attachments of the canola.

[0041] 2. Cut the stem section of the kohlrabi into 15 cm long explants, keep 2 to 3 internodes in each section, and remove the leaves.

[0042] 3. Put the sheared sargassum explants into nutrient solution containing plant hormone Hoagland for cultivation. Keep the temperature at 25°C, control the light intensity and time, cultivate for 10 days, and the roots and shoots will grow about 1 cm long.

[0043] Wherein the preparation method of Hoagland's nutrient solution is basically the same as that of Example 1, except that the addition of gibberellin and indole butyric acid is 5 mg of gibberellin and 4 mg of indole butyric acid;

[0044] 4. Rinse the explants of ...

Embodiment 3

[0047] Embodiment 3: the propagation method of a kind of kohlrabi of the present invention, comprises the steps:

[0048] 1. Collect well-grown and free of diseases and insect pests, and wash 2 to 3 times with clean water to remove the surface attachments;

[0049] 2. Cut the stems of kohlrabi, keep 2 to 3 internodes in each section, about 12 to 15 cm long, and remove the leaves;

[0050] 3. Cultivation conditions of explants: put the cut kohlrabi into Hoagland nutrient solution for cultivation, keep the temperature at 25°C, light intensity of 4000-5000LUX, day ratio of 1:1, After 12 days of cultivation, new roots and shoots will grow;

[0051] Wherein the preparation method of Hoagland's nutrient solution is basically the same as in Example 1, except that the addition of gibberellin and indole butyric acid is 3 mg of gibberellin and 2 mg of indole butyric acid;

[0052] 4. When the roots and buds of the explants grow to 1 cm, rinse them with clean water once and then cut th...

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Abstract

The invention discloses a method for propagating potamogeton malaianus. The method comprises the following steps of: selecting and sterilizing an explant; preparing culture solution and culture conditions; and cutting and managing the explant. The potamogeton malaianus is cultured in Hoagland nutrient solution, the explant is induced to sprout by gibberellins and generate a new root system by indolebutyric acid, and then the potamogeton malaianus explant with induced root and sprout is cut into a seedling culture field and cultivated into a finished product of seedlings. A large number of potamogeton malaianus seedlings can be propagated in a short time period; and the method is easy to operate, effectively avoids the phenomenon that cut seedlings do not root, improves the survival rate of the cut seedlings and meets the engineering requirements because a large number of seedlings can be propagated in a short time period.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for propagating submerged plant sargassum. Background technique [0002] Potamogeton malaianus Miq. Potamogetonaceae, Potamogeton. Perennial submerged floating-leaf herb with fibrous roots at the nodes, and buds on it develop upward into plants with a well-developed underground rhizome system; it grows in lakes, ponds and rivers with relatively hard geology. In my country, except for a few provinces such as Shanxi, Gansu, and Ningxia, it is distributed in other provinces. In many lakes, belacans used to be the dominant species, but with the intensification of eutrophication of water bodies, belacans gradually disappeared, and now it is difficult to find belacans in rivers and lakes. It is imminent to restore the water ecosystem and rebuild the populations of S. japonica. At present, the seedlings of kohlrabi are mainly collected from natural rivers and lakes, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00
Inventor 柴夏马亦兵刘平平周志岭蔡玉巧史加达刘从玉万陆军谢超
Owner 南京中科水治理股份有限公司
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