Application of jerusalem artichokes in repairing cadmium (Cd) polluted environment
A technology that pollutes the environment and cadmium pollution is applied in the field of biological treatment of pollutants, which can solve the problems of limited absorption and accumulation capacity of heavy metals, limited biomass, and environmental pollution.
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Embodiment 1
[0017] Using Jerusalem artichoke (Nanyu No. 1) as the material, the Jerusalem artichoke tubers are rinsed with tap water and distilled water, and then surface sterilized with 0.05% fungicide. The sterilized Jerusalem artichoke tubers with bud-eyes are sown in a plastic pot with quartz sand and a hole in the lower part of the turnover box. The turnover box contains a certain depth of 1 / 2 Hoagland's culture solution. The culture medium was changed every other day, and the quartz sand was fully rinsed each time to ensure the uniformity of the culture conditions and natural light. After the tubers germinate, use 1 / 2 Hoagland’s nutrient solution (2.5mM Ca(NO 3 ) 2 , 2.5mM KNO 3 , 0.5mM KH 2 PO 4 , 0.5mM MgSO 4 , 25μMH 3 BO 3 , 2.25μM MnCl 2 , 1.9μM ZnSO 4 , 0.15μM CuSO 4 , 0.05mM(NH 4 ) 6 Mo 7 O 24 , 5μMFeEDTA, the same below) watering. At the three-leaf stage, thin seedlings are transplanted, one tree per pot is reserved, and three pots are treated for each. Treat at six-leaf stag...
Embodiment 2
[0019] Using Jerusalem artichoke (Nanyu No. 1) as the material, the culture conditions are the same as above, and it is processed at the six-leaf stage, setting 25mg L -1 Cd concentration, directly add the solid CdCl 2 ·2.5H 2 Dissolve O in 1 / 2 Hoagland’s nutrient solution, stir and mix well, and pour. Replace 1 / 2 Hoagland’s culture medium at intervals of the treatment period. Take care not to let the nutrient solution get on the above-ground part of the plant when replacing it. Take samples for testing after three weeks of treatment. The results are as follows: leaf length 7.47cm, leaf width 3.13cm, plant height 15.90cm, root length 12.37cm, root dry weight 0.18g, above ground dry weight 0.51g, whole plant dry weight 0.69g, root Cd concentration 1344.70mg / kg , The Cd concentration in the stem is 174.79mg / kg, the Cd concentration in the leaves is 141.90mg / kg, the root Cd accumulation is 0.24mg, the stem Cd accumulation is 0.09mg, and the leaf Cd accumulation is 0.04mg.
Embodiment 3
[0021] Using Jerusalem artichoke (Nanyu No. 1) as the material, the culture conditions are the same as above, and it is processed at the six-leaf stage, setting 50mg L -1 Cd concentration, directly add the solid CdCl 2 ·2.5H 2 Dissolve O in 1 / 2 Hoagland’s nutrient solution, stir and mix well, and pour. Replace 1 / 2 Hoagland’s culture medium at intervals of the treatment period. Take care not to let the nutrient solution get on the above-ground part of the plant when replacing it. Take samples for testing after three weeks of treatment. The results are as follows: leaf length 7.80cm, leaf width 2.80cm, plant height 15.77cm, root length 11.93cm, root dry weight 0.22g, above ground dry weight 0.64g, whole plant dry weight 0.86, root Cd concentration 2167.90mg / kg, The Cd concentration in the stem was 331.20 mg / kg, the Cd concentration in the leaf was 328.77 mg / kg, the Cd accumulation in the root was 0.48 mg, the Cd accumulation in the stem was 0.21 mg, and the Cd accumulation in the ...
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