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Peanut somatic embryo induction and plant regeneration method

A technique for peanuts and plants, applied in plant regeneration, botany equipment and methods, horticultural methods, etc., can solve the problem of low frequency of plant regeneration, and achieve the effect of avoiding chimerism and high regeneration rate

Inactive Publication Date: 2012-08-22
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for induction of somatic embryos of peanuts and regeneration of plants, which can solve the problem of low frequency of regeneration of plants in the method of induction of somatic embryos of peanuts and regeneration of plants in the prior art

Method used

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  • Peanut somatic embryo induction and plant regeneration method
  • Peanut somatic embryo induction and plant regeneration method
  • Peanut somatic embryo induction and plant regeneration method

Examples

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Effect test

Embodiment 1

[0021] A method for peanut somatic embryo induction and plant regeneration, including the following steps:

[0022] 1 Preparation of somatic embryo induction medium:

[0023] Select MSB 5 (MS Inorganic Salt+B 5 Organic component) is a minimal medium, with 3% sucrose and 0.8% agar added to MSB 5 The pH value of the culture medium is adjusted to 5.8, and the culture room temperature is 24~26℃, the light intensity is 2500~3000Lx, and the daily light is 13h. The MSB 5 Add 2,4-D to the medium to form a somatic embryo induction medium. The amount of 2,4-D added is MSB per liter 5 Add 5mg to the culture medium. 2,4-D is a chlorinated phenoxyacetic acid herbicide. It can also be used as a plant growth regulator. It is a commonly used auxin for inducing callus formation.

[0024] 2Select the explant material:

[0025] The test materials are Huayu 20, Huayu 22, Huayu 23 and Huayu in the series of new peanut varieties selected and promoted by the Shandong Peanut Research Institute (formerly the P...

Embodiment 2

[0036] A method for peanut somatic embryo induction and plant regeneration, including the following steps:

[0037] 1 Preparation of somatic embryo induction medium:

[0038] Select MSB 5 Is the basic medium, add 3% sucrose, 0.8% agar, MSB 5 The pH value of the culture medium is adjusted to 5.8, and the culture is carried out under the conditions of 24~26℃, light intensity of 2500~3000Lx and daily light of 10~15h. 5 Add 2,4-D to the medium to form a somatic embryo induction medium. The amount of 2,4-D added is MSB per liter 5 Add 10 mg to the medium.

[0039] 2Select the explant material:

[0040] The test materials are Huayu 20, Huayu 22, Huayu 23 and Huayu in the series of new peanut varieties selected and promoted by Shandong Peanut Research Institute (formerly the Peanut Research Institute of Chinese Academy of Agricultural Sciences). Yu No. 25 and a new peanut variety Luhua No. 11, which was selected and promoted by Qingdao Agricultural University (formerly Laiyang Agricultural C...

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Abstract

The invention provides a peanut somatic embryo induction and plant regeneration method, which can solve the problem of low plant regeneration frequency in the prior art. The method of the invention comprises the following main steps: separating embryonic leaflet from a peanut embryo which is treated by surface sterilization and soaked, and inoculating the embryonic leaflet to an induction culture medium to which 2,4-D (5-10mg / L) is added for culturing a somatic embryo; then transferring the somatic embryo to a somatic embryo germinating culture medium to which BAP (4mg / L) is added, and subculturing twice to obtain regenerated plants; and cutting the somatic embryo at the bottom after the somatic embryo germinates and elongates, and transferring the cut somatic embryo to a 1 / 2MS inorganic salt and B5 organic rooting culture medium to which NAA (0.2-0.5mg / L) is added for inducing rooting to obtain complete plants. By taking the embryonic leaflet of peanut mature seeds as explant, and using the induction culture medium of 2,4-D (5-10mg / L) and the somatic embryo germinating culture medium of BAP (4mg / L), the somatic embryo induction rate and plant regeneration rate are high, and simultaneously the somatic embryo is derived from one cell, and the chimerism of regenerated plants is avoided.

Description

Technical field [0001] The invention relates to a peanut breeding method, in particular to a method for inducing peanut somatic embryos and plant regeneration. Background technique [0002] Peanuts are important oil crops and economic crops in my country, and they play an important role in agriculture and the entire national economy. However, due to the narrow genetic basis and poor resistance of peanut cultivars, they are especially susceptible to various diseases and insect pests, which seriously affect their yield and quality. Although breeders try to breed stress-resistant varieties, they lack resources of highly resistant varieties in peanut cultivars. Therefore, the breeding of disease-resistant varieties has always been a difficult problem to solve. At present, genetic transformation of resistance genes and in vitro mutation breeding have received extensive attention at home and abroad. Whether gene transformation and in vitro mutagenesis can be successfully applied to f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王晶珊赵明霞隋炯明乔利仙盖树鹏郭宝太王晓杰
Owner QINGDAO AGRI UNIV
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