Method for early diagnosing acute kidney injury caused by contrast medium by utilizing MBL (mannan-binding lectin)

A technology for acute kidney injury and early diagnosis, applied in the medical field, can solve problems such as poor specificity and delay in diagnosis time

Inactive Publication Date: 2011-03-30
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for early diagnosis of acute kidney injury caused by contrast medium using MBL to solve the technical problems of time lag in diagnosis and poor specificity in the prior art

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Embodiment Construction

[0006] Nowadays, there are more and more opinions that SCr is not a sensitive, specific and reliable diagnostic index for AKI. The study of new diagnostic markers can provide the basis for early diagnosis, early intervention and guiding treatment. Compared with serum and other body fluid samples, urine has a relatively simple protein composition, good stability, and easy analysis. It can be used for the detection of various human diseases, especially urinary system diseases. It is a better clinical specimen than serum.

[0007] At present, the applicant has found that urinary interleukin-18 (IL-18) and urinary neutrophil gelatinase-associated apolipoprotein (NGAL) may be used as early diagnostic markers of AKI, but the urine mannose-binding lectin For acute kidney disease, especially the diagnostic value of contrast agent-induced acute kidney injury, there is no related report.

[0008] Therefore, the applicant proposes a method for early diagnosis of acute kidney injury caus...

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Abstract

The invention relates to a method for early diagnosing an acute kidney injury caused by a contrast medium by utilizing urine MBL (mannan-binding lectin), which comprises the following steps of: comparing fluorescence difference electrophoresis charts of the urine of a patient 24 hours after an operation and the basis urine of the patient before the operation, and searching out a plurality of differentially expressed protein spots; identifying through a matrix-supported laser desorption ionization time of flight mass spectrometry technique to discover that the mannan-binding lectin (MBL) and serine protease related to the MBL are both increased in the differentially expressed protein spots, and the difference has a statistical significance for prompting that the acute kidney injury caused by the contrast medium is related to a pathway of MBL (mannan-binding lectin) complement activation, which is verified by a patient with the kidney injury caused by the contrast medium through an urine ELISA (enzyme-linked immuno sorbent assay) way. If applied to the clinic, the method of the invention can be used for detecting the urine MBL (mannan-binding lectin) 24 hours after an operation through the ELISA (enzyme-linked immuno sorbent assay) way and early diagnosing the kidney injury caused by the contrast medium, which is 24 hours earlier than the diagnosis of serum creatinine, and provides basis for early clinical diagnosis, early treatment and improvement of prognosis.

Description

technical field [0001] The present invention relates to the field of medicine, in particular to a method for early diagnosis of acute kidney injury caused by contrast medium, which can be directly applied to medical clinical examination, using MBL (Mannose binding lectin, MBL). Background technique [0002] With the wide application of contrast technology in clinical practice, especially in elderly patients with severe comorbidities, contrast induced nephropathy (CIN) has become the third leading cause of hospital-acquired renal failure, accounting for 11% of the rate. In the United States, there are about one million angiography operations per year, and the incidence of CIN is 150,000 times per year; taking at least 1% of patients requiring dialysis treatment as an example, the average length of hospital stay for patients who do not require dialysis is 2 days, Assuming a daily cost of $500, the annual increase in medical expenses is as high as $148,000,000. Once renal fai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N27/62G01N33/53C12Q1/37
Inventor 倪兆慧王玲贾明宏
Owner 贾明宏
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