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Method for fermenting to produce tremellan by staged pH value control policy

A technology of tremella polysaccharide and stages is applied in the field of staged pH value control strategy fermentation and production of tremella polysaccharide, and achieves the effects of improving yield and simple process

Inactive Publication Date: 2013-07-24
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using the three-stage pH value control method to improve the yield of tremella polysaccharides has not been reported in the literature.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Embodiment 1: fermenter fermentation adopts three-stage culture method, and all the other conditions are the same as the comparative example. In the first stage of fermentation (0-72h), the pH value is controlled at 4.0, in the second stage of fermentation (72-96h), the pH value is controlled at 6.0, and in the third stage of fermentation 96-120h, the pH value is maintained at 7.0, using this three-stage pH value The control strategy fermented and produced tremella polysaccharide, and its yield was increased to 2.0g / L.

Embodiment 2

[0008] Embodiment 2: Fermentation in fermenter adopts three-stage culture method, and other conditions are the same as the comparative example. In the first stage of fermentation (0-72h), the pH value is controlled at 5.0, in the second stage of fermentation (72-96h), the pH value is controlled at 6.0, and in the third stage of fermentation 96-120h, the pH value is maintained at 7.0, using this three-stage pH value The control strategy fermented and produced tremella polysaccharide, and its yield increased to 2.6g / L.

Embodiment 3

[0009] Embodiment 3: Fermentation in fermenter adopts three-stage culture method, and other conditions are the same as the comparative example. In the first stage of fermentation (0-72h), the pH value is controlled at 4.5, in the second stage of fermentation (72-96h), the pH value is controlled at 6.0, and in the third stage of fermentation 96-120h, the pH value is maintained at 6.5, using this three-stage pH value The control strategy fermented and produced tremella polysaccharide, and its yield increased to 2.9g / L.

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Abstract

The invention relates to a method for fermenting to produce tremellan by a staged pH value control policy, belonging to the technical field of optimized application of a pH control technology in a fermenting process. The method comprises the following steps of: controlling the pH value to be 5.0 in a first fermenting stage (0-72 hours); controlling the pH value to be 6.0 in a second fermenting stage (72-96 hours); and maintaining the pH value to be natural in a third fermenting stage (96-120 hours). By adopting the three-stage pH value control process to ferment to produce the tremellan, the output can be improved to 3.8g / L, while the output of a contrast embodiment is 1.5g / L. The tremellan three-stage pH value control technology provided by the invention has the advantages of simple and efficient fermenting process and important industrial application value and has certain guiding and enlightening importance to the fermented production of other fungus polysaccharose.

Description

technical field [0001] The invention discloses a method for fermenting and producing tremella polysaccharide with a strategy of pH value control in stages, and relates to the technical field of the application of pH control technology in optimizing the fermentation process. Background technique [0002] The main medicinal ingredients of white fungus are polysaccharides, mainly including acid polysaccharides, neutral heteropolysaccharides, acid oligosaccharides, cell wall polysaccharides, etc. Tremella polysaccharide has the functions of strengthening the heart, delaying aging, anti-thrombosis, lowering blood fat, lowering blood sugar, anti-hepatitis, anti-mutation, anti-tumor, improving immunity, and anti-radiation. Tremella polysaccharide has a wide range of applications in medicine, beauty, health food and other fields. Tremella polysaccharides can be isolated from Tremella fruiting bodies, and can also be produced by submerged fermentation of conidia. Due to the low cos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 朱虎曹春旭
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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