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Method for preparing pulsatilla chinensis (Bunge) Regel saponin matter and application of pulsatilla chinensis (Bunge) Regel saponin matter in preparation of antischistosomal medicament

An anti-schistosomiasis, Pulsatilla technology, applied in the fields of medicine and veterinary medicine

Active Publication Date: 2011-05-25
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, Pulsatillae water extract and alcohol extract are mixed with some ineffective substances in the mixture, and large doses are required to have sufficient anti-schistosomiasis activity

Method used

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  • Method for preparing pulsatilla chinensis (Bunge) Regel saponin matter and application of pulsatilla chinensis (Bunge) Regel saponin matter in preparation of antischistosomal medicament
  • Method for preparing pulsatilla chinensis (Bunge) Regel saponin matter and application of pulsatilla chinensis (Bunge) Regel saponin matter in preparation of antischistosomal medicament
  • Method for preparing pulsatilla chinensis (Bunge) Regel saponin matter and application of pulsatilla chinensis (Bunge) Regel saponin matter in preparation of antischistosomal medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1, 3-O-α-L-rhamnopyranose-(1→2)-α-L-arabinopyranosyl hedera saponin 28-O-α-L-rhamnopyranose- The preparation method of (1→4)-β-D-glucopyranose-(1→6)-β-D-glucopyranoside is:

[0035] Step 1: Grind Pulsatilla pulsatillae medicinal material into coarse powder, soak in 0-95% ethanol for 0.5-2h, heat and reflux extract 6-12 times the amount for 2-3 times, each time for 1-3h, filter, and combine the filtrates. The above-mentioned filtrate is concentrated under reduced pressure at 50-70°C to 0.5-1.0g raw medicinal materials / mL, refrigerated overnight at 0-4°C, separated from the gel, filtered, and the filtrate is dried by spray drying, vacuum microwave drying or other methods, and collected Dry extract or spray dry powder to get Pulsatilla pulsatilla extract.

[0036] In the second step, the extract of Pulsatilla pulsatillae is dissolved in water, adsorbed by D101 or AB-8 or ADS-17 macroporous resin, eluted with water, 35-45% ethanol, and 95% ethanol in sequence, and ...

Embodiment 2

[0044] Example 2, 3-O-α-L-rhamnopyranose-(1→2)-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranose- The preparation method of (1→4)-β-D-glucopyranose-(1→6)-β-D-glucopyranoside is:

[0045]Step 1: Grind Pulsatilla pulsatillae medicinal material into coarse powder, soak in 70% ethanol for 0.5h, heat and reflux extracting 12 times the amount twice, 3h each time, filter, and combine the filtrates. Concentrate the above filtrate at 50°C to 0.5g raw medicinal materials / mL, refrigerate overnight at 0°C, separate the gel, filter, dry the filtrate by spray drying, vacuum microwave drying or other methods, and collect the dry extract or spray dry powder , that is, Pulsatilla extract.

[0046] In the second step, the Pulsatilla pulsatilla extract is dissolved in water, adsorbed by D101 macroporous resin, eluted with water, 40% ethanol, and 95% ethanol in sequence, and the 40% ethanol eluted part is collected. Anti-schistosomiasis pharmacological experiments prove that the eluted p...

Embodiment 3

[0054] Example 3, 3-O-α-L-rhamnopyranose-(1→2)-α-L-arabinopyranosyl hedera saponin 28-O-α-L-rhamnopyranose- The preparation method of (1→4)-β-D-glucopyranose-(1→6)-β-D-glucopyranoside is:

[0055] Step 1: Grind Pulsatilla pulsatillae medicinal material into coarse powder, soak in 60% ethanol for 2 hours, heat and reflux extract 6 times the amount for 33 times, each time for 1 hour, filter, and combine the filtrates. The above filtrate was concentrated under reduced pressure at 70°C to 1.0g raw medicinal materials / mL, refrigerated overnight at 4°C, separated from the gel, filtered, and the filtrate was dried by spray drying, vacuum microwave drying or other methods, and the dry extract or spray-dried powder was collected , that is, Pulsatilla extract.

[0056] In the second step, the Pulsatilla pulsatilla extract is dissolved in water, adsorbed by AB-8 macroporous resin, eluted with water, 35% ethanol, and 95% ethanol in sequence, and the eluted part of 35% ethanol is collecte...

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Abstract

The invention relates to a method for preparing a pulsatilla chinensis (Bunge) Regel saponin matter and application of the pulsatilla chinensis (Bunge) Regel saponin matter in preparation of an antischistosomal medicament. The pulsatilla chinensis (Bunge) Regel saponin matter is a single-component compound, and the method for preparing the pulsatilla chinensis (Bunge) Regel saponin matter comprises the following steps of: grinding the pulsatilla chinensis (Bunge) Regel medicinal materials into coarse powder, soaking by using ethanol, performing reflux extraction, condensing and drying, collecting dry extract, dissolving the dry extract in water, performing macroporous resin adsorption, and collecting the ethanol eluation part; and treating by using an opposite phase silica gel column, and collecting the eluation part of methanol and water to obtain the single-component compounds. According to the comparison with a comparison product and the LC / MS / MS test, the compounds are 3-O-alpha-L-rhamnopyranosyl-(1-2)-alpha-L-arabinopyranosyl-hederagenin28-O-alpha-L-rhamnopyranosyl-(1-4)-beta-D-glucopyranose-(1-6)-beta-D-glucopyranoside.

Description

technical field [0001] The invention belongs to the technical field of medicine and veterinary medicine, and relates to the application of Pulsatilla saponins in the preparation of anti-schistosomiasis drugs and the preparation method of the substance. The effective components have significant anti-schistosomiasis activity and can be used for human or other mammals Prevention and treatment of animal schistosomiasis. Background technique [0002] Pulsatilla medicinal material is taken from the dried root of Pulsatilla chinensis (Bunge) Regel, a plant of the genus Pulsatilla in the family Ranunculaceae, which is bitter in taste and cold in nature. Return stomach, large intestine meridian, tool heat-clearing and toxic substances removing, the merit of cooling blood and stopping dysentery. For pyretotoxic blood dysentery, pruritus vulvae leukorrhagia, amoebic dysentery. According to literature reports, Pulsatillae contains Protoanemonin, Pulsatillaein (Anemonin), Pulsatillaein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P33/12C07J63/00C07H15/256C07H1/08
CPCY02A50/30
Inventor 杨世林李孝贤许琼明夏超明朱卫丰刘艳丽简辉罗晓健李笑然冯育林
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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