Device and method for preparing stem cells by continuous perfusion bioreactor/tank bag system

Abstract

The invention relates to a device for preparing stem cells through a continuous perfusion bioreactor / culture bottle (bag) system. The device comprises a culture solution storage tank / bag, a monitored bioreactor, a central cell culture warehouse, a waste liquor / obtained liquor collecting container, physicochemical and biochemical indicator detecting head groups, an online monitoring system and a sterile filter membrane filter, which are connected by a speed-controlled pipeline system of a fluid-controlled sterile driver. The connecting relationships are that: the culture solution storage tank / bag is connected with the monitored bioreactor through the speed-controlled pipeline system; the monitored bioreactor is connected with an inlet of the central cell culture warehouse through the speed-controlled pipeline system; an outlet of the central cell culture warehouse is connected with the waste liquor / obtained liquor collecting container through the speed-controlled pipeline system; the monitored bioreactor is connected to the online monitoring system through a physicochemical and biochemical indicator detecting head group of the reactor; and the online monitoring system is connected with the physicochemical and biochemical indicator detecting head groups at the inlet and outlet of the central cell culture warehouse respectively.

Description

technical field [0001] The present invention relates to a device and method for preparing stem cells in a continuous perfusion bioreactor and a culture bottle / bag system in the field of bioengineering, in particular to a continuous perfusion bioreactor and a culture bottle / bag system that are valuable but difficult to grow Devices and methods for stem cells. Background technique [0002] Judging from the current progress of tissue engineering, the use of stem cells to treat tissue and organ damage and necrosis is the most promising treatment method, and its medical effect and economic return expectations are considerable. So far, stem cells (such as mesenchymal stem cells) are representative of the therapeutic cell culture preparation method. Traditionally, the stem cells are extracted and inoculated on a plate or T bottle, and placed in a 37°C, saturated humidity, 5% carbon dioxide incubator. Perform static discontinuous feed culture. However, the preparation of stem cell...

AI Technical Summary

Problems solved by technology

However, similar studies only focus on the problem of limited single factors in the process of stem cell expansion, and have not been able to use continuous perfusion bioreactors combined with effective monitoring methods to perform efficient, continuous, controllable, and engineerable amplification of stem cells. amplified preparation

Images