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Establishing method of hepatocyte apoptosis animal model and application thereof

A technology of animal model and method establishment, applied in the direction of medical preparations containing active ingredients, biological tests, drug combinations, etc., can solve the problems of lack of liver cell apoptosis models and difficulties in the development of new anti-hepatitis drugs for hepatitis treatment strategies, etc. Achieve good correlation, reduce liver inflammation and pathological damage, and consume less manpower and financial resources

Inactive Publication Date: 2011-08-03
伍义行
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still a lack of stable and reliable hepatocyte apoptosis models available, which has caused great difficulties in the expansion of hepatitis treatment strategies and the development of new anti-hepatitis drugs

Method used

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  • Establishing method of hepatocyte apoptosis animal model and application thereof
  • Establishing method of hepatocyte apoptosis animal model and application thereof
  • Establishing method of hepatocyte apoptosis animal model and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Preparation and Evaluation of Hepatocyte Apoptosis Mouse Model

[0015] 1 Prepare the following materials:

[0016] 1.1 Experimental animals: ICR mice were provided by Zhejiang Experimental Animal Center.

[0017] 1.2D-Galactosamine: a product of Jiaxing Hengjie Biological Engineering Co., Ltd.

[0018] 1.3 Lipopolysaccharide: product of SIGMA Company of the United States.

[0019] 1.4 Cell apoptosis DNALadder detection kit: Nanjing KGI Biotechnology Development Co., Ltd.

[0020] 1.5 Apoptosis fluorescent Hoechst33342 / PI double staining kit: Nanjing KGI Biotechnology Development Co., Ltd.

[0021] 1.6 In Situ Cell Death Detection Kit, POD: Roche Kit

[0022] 2 The preparation method is as follows:

[0023] 2.1 The mice were adaptively fed for 3 days before the experiment.

[0024] 2.2 Prepare the apoptosis inducer D-galactosamine and lipopolysaccharide solution with physiological saline according to the formula ratio determined by screening.

[0025] ...

Embodiment 2

[0029] Example 2: Evaluation of anti-apoptotic drugs

[0030] 1 Prepare the following materials:

[0031] 1.1 Experimental animals: ICR mice were provided by Zhejiang Experimental Animal Center.

[0032] 1.2D-Galactosamine: a product of Jiaxing Hengjie Biological Engineering Co., Ltd.

[0033] 1.3 Test liquid (diluted with normal saline)

[0034] 1.4 Lipopolysaccharide: product of SIGMA Company of the United States.

[0035] 1.5 Apoptosis DNA Ladder Detection Kit: Nanjing KGI Biotechnology Development Co., Ltd.

[0036] 2 The evaluation method is as follows:

[0037] 2.1 ICR mice were randomly divided into three dose groups, large, medium and small, and a solvent control group, with 10 mice in each group.

[0038] 2.2 Preventive administration three days before modeling, once a day; administration once a day after modeling, and 4 hours after the last administration, the mice were sacrificed, serum was taken to measure Caspase-3 activity by ELISA, and liver tissue was take...

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Abstract

The invention relates to a preparation method of a hepatocyte apoptosis animal disease model by coinduction of D-galactosamine and lipopolysaccharide, in particular to a simple inducer formula and a simple operation process which can generate a hepatocyte apoptosis rate above 80% and reproduce a stable and reliable hepatocyte apoptosis animal model. The invention also relates to the application of the hepatocyte apoptosis animal model to the research on the pathogenesis of hepatitis and evaluation of novel anti-hepatitis medicaments.

Description

1. Technical field [0001] The invention relates to a preparation method of an animal disease model, in particular a simple inducer formula and a simple operation procedure, which can produce a liver cell apoptosis rate of more than 80%, and reproduce a stable and reliable liver cell apoptosis animal model And its application in the study of the pathogenesis of hepatitis and the evaluation of new anti-hepatitis drugs. 2. Background technology [0002] Hepatitis is a general term for inflammatory diseases of the liver. According to the etiology, it can be divided into viral, alcoholic, drug-induced and autoimmune hepatitis. Viral hepatitis is the most common, of which hepatitis B is the main one, and hepatitis C next. Hepatitis B is a very harmful worldwide epidemic infectious disease caused by hepatitis B virus (HBV). According to statistics, there are currently about 350 million HBV carriers in the world, and China is a high-infection area of ​​HBV. HBV carriers Patients a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K31/7008A61P1/16A61P31/12G01N33/50
Inventor 胡少青伍义行郝冰洁姜威张虹莉贾袁媛
Owner 伍义行
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