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Application of long-chain neurotoxin of cobra in preparation of muscarine type M4 receptor activator

A technology of receptor activators and neurotoxins, which can be used in nervous system diseases, anti-inflammatory agents, pharmaceutical formulations, etc., and can solve the problem of no reports on choline receptors

Inactive Publication Date: 2011-08-03
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on which choline receptor and its receptor subtype

Method used

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  • Application of long-chain neurotoxin of cobra in preparation of muscarine type M4 receptor activator
  • Application of long-chain neurotoxin of cobra in preparation of muscarine type M4 receptor activator
  • Application of long-chain neurotoxin of cobra in preparation of muscarine type M4 receptor activator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] This example uses behavioral and whole-cell voltage clamp methods to record the effects of cobra long-chain neurotoxin (α-CbTX) on mouse behavior and T-type calcium currents in dorsal root neurons, and identify the participating cobra long-chain neurotoxins (α-CbTX) receptor subtype for analgesia.

[0020] 1) Identification of choline receptors in mouse dorsal root ganglion (DRG)

[0021] Experimental Materials

[0022] Kunming mice, weighing 18-22g, were provided by Suzhou Animal Center, animal certificate number: SYXK (Su) 2007-0035.

[0023] Experimental program

[0024] 5 mice were taken, DRG was isolated under microsurgical operation, RNA was extracted, cDNA was obtained by reverse transcription method, and the corresponding primers of the four subtype receptors of choline were used for amplification. Such as figure 1 Shown is the expression of muscarinic M1R, M2R, M3R and M4R receptors in mouse dorsal root ganglia. figure 1 M is the ladder, DRG is the dorsal ...

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Abstract

The invention discloses application of long-chain neurotoxin (alpha-cobratoxin, alpha-CbTX) of cobra in preparation of a muscarine type M4 receptor activator. The neurotoxin has the function of reducing pain through activating M4 receptor-dependent T type calcium current, and the muscarine type M4 receptor activator containing the long-chain neurotoxin of the cobra can be established to perform the application of clinical analgesia.

Description

technical field [0001] The invention belongs to the field of new discovery of drug targets, and specifically relates to the application of a cobra long-chain neurotoxin (α-cobratoxin, α-CbTX) in the preparation of a muscarinic M4 receptor activator. The body exerts an analgesic effect. Background technique [0002] Snake venom is a complex mixture of components secreted by snake venom glands. More than 90% of the dry matter of snake venom is protein, which is the main component of its toxicity and its biological activity. Traditional medicine believes that cobra and its poisonous components can clear the meridians, dispel rheumatism, and have the effect of strengthening the body. As early as the beginning of the 20th century, people began to use snake venom to relieve malignant tumor pain, neuralgia and joint pain. A series of reports about snake venom having analgesic effects similar to morphine, which is dependent on analgesia, is a major problem that confuses clinical t...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P25/04A61P29/00
Inventor 陶金秦正红蒋星红张玲
Owner SUZHOU UNIV
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