Porcine trachea epithelial cell line and building method thereof

An epithelial cell and a method for establishing a technology, applied in the field of biological animal cell lines, can solve the problems of high requirements on nutritional conditions, few monoclonal positive cells, and small amount of cell growth.
CN102174573AInactive Publication Date: 2011-09-07张彦明 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
张彦明
Publication Date
2011-09-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a building method of a porcine trachea epithelial cell line. The method comprises the following steps of: (1) primary culture: sterilely acquiring newborn piglet tracheas, clearing adhesive tissues, performing digestion and separation by adopting a pronase perfusion cold digestion method to obtain high-purity trachea epithelial cells, suspending the separated primary trachea epithelial cells in a DF12 culture medium, and culturing the cells at the temperature of 37 DEG C under the condition of 5 percent CO2; (2) transfection and screening: extracting and purifying eukaryotic expression plasmids pCI-neo-hTERT containing hTERT genes, and guiding the eukaryotic expression plasmids pCI-neo-hTERT for coding hTERT and neo genes to the porcine trachea epithelial cell primarily cultured in the step (1) by adopting a liposome transfection method to perform transfection; and (3) screening and amplified culture. The method is easy in culture, the growth speed is high, and the operating method is simple and convenient.
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Description

technical field

[0001] The invention relates to the field of biological animal cell lines, in particular to a porcine tracheal epithelial cell line and a method for establishing the same. Background technique

[0002] The tracheal epithelium is located on the inner surface of the tracheal lumen and is a pseudostratified ciliated columnar epithelium, which is the first barrier for the body to defend against harmful substances. Its constituent cells - tracheal epithelial cells, in addition to having a mechanical defense function, can also synthesize and secrete a variety of mucus components and cytokines, which are used to regulate the growth of airway epithelial cells, maintain the integrity of the tracheal epithelium, moisten and purify the respiratory tract, resist In addition, tracheal epithelial cells also play an important role in participating in the immune response of airway mucosa and helping to eliminate tracheal inflammation.

[0003] At present, there is no resear...

Claims

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