13 results about "Kluyveromyces lactis" patented technology

This invention provides an in vitro cell-free protein synthesis system, its reagent kit, and its applications, belonging to the field of protein synthesis technology. This invention improves an in vitro cell-free protein synthesis system based on Kluyveromyces lactis by adding exogenous L-arabinose, significantly enhancing the system's protein synthesis capacity. Furthermore, it provides a more efficient and higher-throughput in vitro protein synthesis reagent kit and a method for synthesizing exogenous proteins. The improvements provided by this invention can be achieved without molecular modification, are simple and convenient to operate, and save costs.

The invention relates to a composition with sleep aiding and skin repairing effects and a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a composition with sleep aiding and skin repairing effects. The composition is prepared from the following components in parts by weight: 72 to 114 parts of compound plant essential oil, 3 to 5 parts of ceramide NP and 1 to 3 parts of sodium hyaluronate, the compound plant essential oil is prepared from lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil and rhodiola rosea essential oil; the rhodiola rosea essential oil is obtained by fermenting a mixed strain composed of kluyveromyces lactis and trichoderma asperellum and then extracting essential oil. The research shows that the mixed strain fermentation can synergistically improve the extraction rate of the rhodiola rosea essential oil. In addition, the compound plant essential oil provided by the invention can also synergistically improve the anti-inflammatory, moisturizing and anti-allergy repair capabilities of the composition and has the effect of improving sleep disorders, so that the composition has better sleep aiding and skin repairing effects at the same time.

This invention belongs to the field of cosmetic technology and discloses a composition containing polypeptides, its preparation method, and its application. The composition provided by this invention comprises: polypeptides and a solubilizing and penetration-enhancing complex; the polypeptides include at least one selected from Codonopsis pilosula cyclic peptide B, conopeptide, carnosine, acetyl tetrapeptide-5, acetyl hexapeptide-8, and nonapeptide-1; the solubilizing and penetration-enhancing complex comprises the fermentation product filtrate of Kluyveromyces lactis / Ganoderma lucidum fruiting body extract, polyols, polysorbate, and ferulic acid. The composition provided by this invention, through the synergistic effect between its components, can effectively improve the solubility and stability of the polypeptides, preventing precipitation or exudation, while effectively promoting the transdermal absorption of the polypeptides; the composition provided by this invention has excellent antioxidant and anti-aging effects.

The present invention relates to a fermented dairy product comprising the microorganisms S. thermophilus and L. kefiri,, and which can further comprise a yeast selected from Lachancea sp. and Kluyveromyces lactis. The invention further relates to a method for obtaining said dairy product and food and beverages containing same.

This invention relates to a multi-effect synergistic lipid-lowering composition for food and its preparation method, belonging to the field of food technology. The composition includes the following raw materials: a compound fermentation broth, *Gynostemma pentaphyllum* extract, *Lycium chinense* root bark extract, and *Rosa rugosa* fruit extract. In the preparation of the compound fermentation broth, *Rosa laevigata*, mulberry twigs, and *Fritillaria cirrhosa* are co-fermented with a compound microbial culture consisting of *Pediococcus pentosaceus*, *Lactobacillus curvatureii*, and *Kluyveromyces lactis*. This fully utilizes the synergistic effects of the raw materials and the advantages of the fermentation process, further enhancing the antioxidant activity of the multi-effect synergistic lipid-lowering composition. Furthermore, this invention also uses *Gynostemma pentaphyllum*, *Lycium chinense* root bark, and *Rosa rugosa* fruit as raw materials to prepare extracts, which are then compounded as components of the multi-effect synergistic lipid-lowering composition. This effectively utilizes the lipid-lowering properties of different raw materials, synergistically enhancing their effects to achieve lipid-lowering and weight-loss effects.

The application discloses a method for constructing a genetically engineered Kluyveromyces lactis strain for producing miR162a, which comprises co-transferring two plasmids expressing DCL1, HYL1 and SE genes from Arabidopsis thaliana into a Kluyveromyces lactis GG799 strain by electroporation, so that the yeast obtains the ability to cut plant-derived precursor miRNA, one of the plasmids synchronously expresses a precursor of miR162a from Lycium ruthenicum Murr, and the genetically engineered Kluyveromyces lactis strain for stably producing miR162a is obtained after acetamide screening. The application further discloses the genetically engineered Kluyveromyces lactis strain constructed according to the above method and application thereof. The genetically engineered strain constructed by the method has a high yield of miR162a at a laboratory flask level, and can stably produce miR162a after continuous subculture, the genetically engineered Kluyveromyces lactis strain exhibits the ability to promote osteogenic differentiation in a cell experiment, and can effectively improve the osteoporosis symptoms of mice in an animal experiment.

This invention provides a natto fermentation composition and its application, belonging to the field of natto fermentation technology. The natto fermentation composition provided by this invention includes *Lactobacillus casei*, *Kluyveromyces lactis*, and *Bacillus natto*. The natto fermentation composition provided by this invention significantly increases nattokinase activity while reducing ammonia odor, making the fermented natto more acceptable to consumers. The natto fermented using the natto fermentation composition and method of this invention has a nattokinase activity as high as 2620.43±4.05 U / mL, and the natto fermented by this invention has a low volatile basic nitrogen content and a high sensory evaluation.

This invention provides a method for preparing low-fat lactose-free milk, belonging to the field of dairy product technology. Based on raw milk, the method involves centrifuging to obtain low-fat milk, adding lactase (produced through aerobic fermentation using Aspergillus oryzae CICC 40228 and Kluyveromyces lactis BNCC 186193 and then immobilizing it) for enzymatic hydrolysis. The milk is then subjected to vacuum degassing, VTIS instantaneous sterilization, and two-stage homogenization to obtain low-fat lactose-free milk. The immobilized lactase prepared by the combined microbial fermentation has high activity and good compatibility, enabling deep lactose hydrolysis without introducing undesirable flavors. Vacuum degassing removes gases and trace amounts of off-flavors from the milk base and prevents oxidative denaturation. VTIS instantaneous sterilization achieves commercial sterility while maximizing the preservation of milk nutrients and natural flavor. Two-stage homogenization optimizes the low-fat milk colloidal dispersion system through the synergistic effect of high-pressure crushing and low-pressure stabilization, solving the problems of fat floating and protein precipitation.

The invention belongs to the technical field of enzyme engineering and amino acid biosynthesis, and particularly discloses an alpha-amino adipic acid-delta-semialdehyde dehydrogenase mutant and application thereof. The invention provides a series of alpha-amino adipic acid-delta-semialdehyde dehydrogenase mutants with excellent catalytic activity on L-2-amino adipic acid-delta-semialdehyde by carrying out directional modification on alpha-amino adipic acid-delta-semialdehyde dehydrogenase derived from kluyveromyces lactis. The invention further provides a preparation method of the alpha-amino adipic acid-delta-semialdehyde dehydrogenase mutants. By taking the mutant as a catalyst, the invention provides a biosynthesis method of L-2-aminoadipic acid, and the method can increase the substrate conversion rate to 84.93%-95.27%, so that the utilization rate of raw materials is remarkably increased, the production cost of the raw materials is reduced, and the yield of L-2-aminoadipic acid is increased. The defects of long production route, high cost, harsh reaction conditions, low yield, serious pollution and the like in a chemical synthesis route are also avoided, and green, efficient and low-cost preparation of the L-2-aminoadipic acid is realized.

The invention belongs to the technical field of biology, and discloses kluyveromyces lactis FRI and application of lysate thereof in preparation of weaned piglet feed additives or application of lysate thereof in preparation of weaned piglet feeds, and the preservation number of the kluyveromyces lactis FRI is CGMCC No.30910; the preservation date is June 24, 2024, the classification name is Kluyveromyces lactis, the preservation unit is China General Microbiological Culture Collection Center, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Kluyveromyces lactis and lysate thereof are added into the feed, so that the growth performance of weaned piglets can be improved, and the diarrhea rate can be reduced.

The invention relates to a beta-galactosidase mutant derived from Kluyveromyces lactis and application of the beta-galactosidase mutant, and belongs to the technical field of enzyme engineering and genetic engineering. A series of mutants are obtained by performing molecular modification on beta-galactosidase from kluyveromyces lactis, and the beta-galactosidase mutant L764T of which the catalytic activity is remarkably improved is screened by verifying hydrolytic activity and transglycosylation activity. Compared with a wild enzyme, the mutant enzyme has the advantages that the catalytic efficiency is improved to 2.9 times, the specific enzyme activity is improved to 3.36 times, the transglycosylation efficiency is improved by 36.4%, and the thermal stability is also improved. Based on the advantages of beta-galactosidase from Kluyveromyces lactis in the aspects of safety, food industry adaptability and the like, the mutant enzyme disclosed by the invention has a wide application prospect, and breaks through the limitation of raw materials used in the current dairy product preparation industry; the method is expected to be used in the production of lactose-free dairy products or low-lactose dairy products and the synthesis of galactooligosaccharides.

The invention belongs to the technical field of fermentation engineering, and particularly relates to roselle fermentation liquor with a weight reducing effect. The preparation method of the roselle fermentation liquor comprises the following steps: pretreating roselle, adding nutritional factors, and uniformly mixing to obtain a fermentation substrate; adding kluyveromyces marxianus liquid, kluyveromyces lactis liquid and lactobacillus helveticus liquid for fermentation, and filtering after fermentation to obtain roselle fermentation liquid; the nutritional factors comprise inulin and linoleic acid. Experiments prove that the roselle fermentation liquor provided by the invention can effectively reduce the body fat rate of rats and has a weight reducing effect.

The invention relates to a beta-galactosidase mutant with high transglycosylation activity and thermal stability and application of the beta-galactosidase mutant, and belongs to the technical field of enzyme engineering and genetic engineering. According to the invention, beta-galactosidase mutants V842G and L764T / V842G with significantly improved catalytic activity and thermal stability are obtained by carrying out molecular modification on beta-galactosidase from Kluyveromyces lactis and screening hydrolytic activity, transglycosylation activity and half-life period at 40 DEG C. The invention also provides a preparation method of the beta-galactosidase mutants V842G and L764T / V842G. Compared with a wild enzyme, the mutant enzyme has the advantages that the catalytic efficiency is improved to 1.5-4.85 times, the specific enzyme activity is improved to 88.9 U / mg-92.3 U / mg, the transglycosylation efficiency is improved by 79.7% at most, and the half-life period under the condition of 40 DEG C is prolonged by 54.7%-101.5%. The beta-galactosidase mutant disclosed by the invention has good performance in lactose degradation and galactooligosaccharide (GOS) synthesis.