Screening method of D-arabitol high-yield yeast mutant strain

An arabitol and mutant strain technology, applied in the field of ion beam, can solve the problem of large randomness of ion implantation technology transformation, and achieve the effect of speeding up the screening speed, improving the reliability and ensuring the screening efficiency

Pending Publication Date: 2014-11-26
SHAANXI UNIV OF SCI & TECH
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Problems solved by technology

However low energy N + The transformation of ion implantation technology is highly random, so it is particularly important to establish a high-throughput rapid screening method

Method used

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  • Screening method of D-arabitol high-yield yeast mutant strain

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Embodiment Construction

[0024] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0025] (1) culture medium

[0026] The composition of the high-sugar solid medium is: glucose 500.0g / L, peptone 20.0g / L, yeast extract 10.0g / L, agar 20.0g / L, pH 6.5;

[0027] The composition of YPD liquid medium is: glucose 20.0g / L, peptone 20.0g / L, yeast extract 10.0g / L, pH 6.5;

[0028] The composition of YPD fermentation medium is: glucose 200.0g / L, peptone 20.0g / L, yeast extract 10.0g / L, pH 6.5.

[0029] The composition of the slant medium is: glucose 20.0g / L, peptone 20.0g / L, yeast extract 10.0g / L, agar 20g / L, pH 6.5;

[0030] (2) The method on which the screening is based

[0031] (1) High-sugar tolerance test: the starting strain implanted with low-energy ions was inoculated on a high-sugar solid medium, cultured at a constant temperature of 37° C. for 48 hours, and the growth of the bacteria was observed.

[0032] (2) Re-screening by paper...

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Abstract

The invention provides a screening method of a D-arabitol high-yield yeast mutant strain. By using a mutant strain obtained by carrying out mutagenesis on Kluyveromyces lactis by a low-energy N+ ion implantation technique as a screening object, a high-flux screening method, which is implemented by primary screening based on a high-sugar solid culture medium and secondary screening based on paper chromatography/high-performance liquid chromatography combination, is established. The method has the advantages of high screening efficiency, low cost, short screening period and the like, is easy to operate, and provides a new method for efficiently screening the D-arabitol high-yield strain by modifying the yeast with low-energy ion implantation.

Description

technical field [0001] The invention belongs to the technical field of ion beams, and in particular relates to a screening method for yeast mutant strains with high D-arabitol production. Background technique [0002] D-arabitol is a five-carbon sugar alcohol with the molecular formula C 5 h 12 o 5 , with a molecular weight of 152.12, is an isomer of xylitol and ribitol. Sugar alcohols are a type of polyols reduced by sugar. They have the advantages of low calorific value, anti-caries, and no effect on insulin levels. They have important application values ​​in the fields of medicine, food, and chemical industry. At present, the conventional method for producing D-arabitol is mainly chemical synthesis, but this method has complex reaction process, high equipment requirements and serious environmental pollution. In view of the shortcomings of the chemical synthesis method, people focus on the production of D-arabitol by the biotransformation method. The reaction condition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12R1/645
Inventor 钱卫东周颖欣王婷宁肖肖蔡长龙毛培宏
Owner SHAANXI UNIV OF SCI & TECH
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