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Antrodia camphorata cyclohexenone compound for suppressing growth of lymphoma tumor cells

A technology of tumor cells and compounds, applied in the field of Antrodia antrodia cyclohexenone compounds used to inhibit the growth of lymphoma tumor cells, can solve unpublished specific problems

Active Publication Date: 2011-11-09
GOLDEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although it can be known from many current experiments that the extract of Antrodia Cinnamomea has the aforementioned effects, and its components have been analyzed one after another, but no specific research has been published on which active ingredients in the extract can contribute to the anticancer effect of Antrodia Cinnamomea. The relevant active ingredients need to be clarified by further experimental research. Therefore, if the ingredients contained in the extract can be found to effectively inhibit tumor growth, it will be beneficial to the research on the mechanism of Antrodia Cinnamomea inhibiting cancer, and the application of Antrodia Cinnamomea to cancer such as lymphatic Cancer treatment and prevention are of great help

Method used

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  • Antrodia camphorata cyclohexenone compound for suppressing growth of lymphoma tumor cells
  • Antrodia camphorata cyclohexenone compound for suppressing growth of lymphoma tumor cells
  • Antrodia camphorata cyclohexenone compound for suppressing growth of lymphoma tumor cells

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separate

[0024] About 100 grams of Antrodia mycelium, fruiting bodies or the mixture of the two are placed in a conical flask, and an appropriate proportion of water and alcohols (70% to 100% alcohol aqueous solution) is added, wherein the alcohol It is preferably ethanol, stirred and extracted at 20-25°C for at least 1 hour, then filtered with filter paper and a 0.45 μm filter membrane, and the filtrate was collected to obtain the Antrodia camphorata extract.

[0025] The Antrodia camphorata extract collected above was analyzed using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP18 chromatographic tube (column), and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B) ) as the mobile phase (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio 10% to 90%; 35 to 4...

Embodiment 2

[0028] Anti-lymphoma tumor cell activity test in vitro

[0029] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , added to the culture medium containing human lymphoma tumor cell U937 to test the viability of tumor cells. Among them, the test of cell viability can be analyzed by conventional MTT assay, and the lymphoma tumor cell U937 is a human histiocytic lymphoma cell line.

[0030] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide) is a yellow dye, which can be absorbed by living cells and absorbed by succin...

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Abstract

The invention relates to a new use of a compound. In the invention, (4-hydroxy-2,3-dimethoxy-6-methy-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone is obtained from extract of Antrodia camphorata by separation and purification, and the cyclohexenone compound can be used for suppressing the growth of lymphoma tumor cells and can also be used in medical composition for suppressing the growth of the lymphoma tumor cells.

Description

technical field [0001] The present invention relates to a new application of a compound, in particular to a use of a compound isolated and purified from the extract of Antrodia cinnamomea to inhibit the growth of lymphoma tumor cells. Background technique [0002] Lymphoma is characterized by the destruction of normal lymphocytes and their replacement by immature lymphocytes. Lymphoma is a malignant tumor that is prone to occur in the lymphatic system in the body, including lymphocytes, lymphatic vessels, and lymph glands; on the other hand, lymphocyte regulatory organs responsible for the production, storage, and division of lymphocytes include bone marrow, spleen, and lymphocytes. Thymus lesions can also induce lymphoma. Clinically, lymphoma is often divided into Hodgkin's lymphoma (Hodgkin's disease) and non-Hodgkin's lymphoma (Non-Hodgkin's lymphoma) according to the different pathological types of tumors. Jerkin's lymphoma is the most common. [0003] Possible causes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00
Inventor 刘胜勇温武哲郭茂田
Owner GOLDEN BIOTECH
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