Isariacicadae strain and use thereof
A technology for C. cicadae and C. cicadae, which is applied to the field of inoculating silkworm to cultivate Cicada and Cic. Cicada strains, and can solve the problems of listing, unsatisfactory yield, unsuitable medicinal materials and the like.
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Embodiment 1
[0027] Ichosporum cicadae of the present invention ( Isaria cicadae ) Separation steps of Mount Qingcheng-16:
[0028] (1) Fresh specimens of cicada nymphs that died of fungal diseases were collected from the bamboo forest of Mount Qingcheng in July 2003;
[0029] (2) Immediately burn the sterilized inoculation needle from the swollen top of the spore bundle of the specimen with an alcohol flame, gently pick a small amount of spore powder and place it on the slanted surface of the sterilized Sabouraud medium, at natural temperature After 3 to 5 days, white colonies covered the slant.
[0030] (3) Medan-stained glass slides were made with transparent tape slime fungus, and examined under a microscope. The spore-forming structure of the screened bacteria was similar to that of I. cicadae ( Isaria cicadae ) kind of similarity, get Isaria cicadae Mount Qingcheng - 16.
[0031] To further illustrate with the following example Isaria cicadae The purpose of Mount Qingcheng-16....
Embodiment 2
[0033] A kind of Cicada Isyphilis ( Isaria cicadae ) Qingcheng Mountain-16 cultivates the method for cicadae, comprises the following steps:
[0034] (1) Will Isaria cicadae ) Qingchengshan-16 uses Capek, modified Capek (Capek + 0.05% protein jelly), Sabourd or potato dextrose agar (PDA) medium to cultivate spore powder at 26-28°C;
[0035] (2) After culturing for 1 week, use 0.05% Tween 80 water to wash the spores of I. cicadae from the slant of the petri dish or test tube and dilute to 1X10 6 to 1X10 8 Conidia / ml concentration of spore suspension;
[0036] (3) Gently put the 5th instar silkworm into the above spore suspension for 2 seconds, pick it up, put it into a sterilized petri dish with absorbent paper, and cultivate it for 3 weeks at room temperature with moisture (RH value above 80%) Get cicadas.
Embodiment 3
[0038] A kind of Cicada Isyphilis ( Isaria cicadae ) Qingcheng Mountain-16 cultivates the method for cicadae, comprises the following steps:
[0039] (1) (2) Synchronously with embodiment 2;
[0040] (3) Gently put the 5th instar silkworm into the above spore suspension for 3 seconds, then pick it up, put it into a sterilized petri dish with absorbent paper, keep it moist at 28°C (RH value above 80%) and cultivate for 3 Zhou got the cicada flower.
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