Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Isariacicadae strain and use thereof

A technology for C. cicadae and C. cicadae, which is applied to the field of inoculating silkworm to cultivate Cicada and Cic. Cicada strains, and can solve the problems of listing, unsatisfactory yield, unsuitable medicinal materials and the like.

Inactive Publication Date: 2011-11-23
GUIZHOU UNIV
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its main production areas are in Jiangsu, Zhejiang, Sichuan, Yun, Gui, Fu, and Guangxi, but the output is far from meeting people's demand for cicadae as medicine and health food
Cicadas cultivated on natural substrates have only sub-seats but no insect bodies. They are quite different from wild cicadas that are compounded with insects and fungi in appearance. Therefore, they are not suitable for marketing as medicinal materials and can only be used as raw materials for processing related medicines or health products.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Ichosporum cicadae of the present invention ( Isaria cicadae ) Separation steps of Mount Qingcheng-16:

[0028] (1) Fresh specimens of cicada nymphs that died of fungal diseases were collected from the bamboo forest of Mount Qingcheng in July 2003;

[0029] (2) Immediately burn the sterilized inoculation needle from the swollen top of the spore bundle of the specimen with an alcohol flame, gently pick a small amount of spore powder and place it on the slanted surface of the sterilized Sabouraud medium, at natural temperature After 3 to 5 days, white colonies covered the slant.

[0030] (3) Medan-stained glass slides were made with transparent tape slime fungus, and examined under a microscope. The spore-forming structure of the screened bacteria was similar to that of I. cicadae ( Isaria cicadae ) kind of similarity, get Isaria cicadae Mount Qingcheng - 16.

[0031] To further illustrate with the following example Isaria cicadae The purpose of Mount Qingcheng-16....

Embodiment 2

[0033] A kind of Cicada Isyphilis ( Isaria cicadae ) Qingcheng Mountain-16 cultivates the method for cicadae, comprises the following steps:

[0034] (1) Will Isaria cicadae ) Qingchengshan-16 uses Capek, modified Capek (Capek + 0.05% protein jelly), Sabourd or potato dextrose agar (PDA) medium to cultivate spore powder at 26-28°C;

[0035] (2) After culturing for 1 week, use 0.05% Tween 80 water to wash the spores of I. cicadae from the slant of the petri dish or test tube and dilute to 1X10 6 to 1X10 8 Conidia / ml concentration of spore suspension;

[0036] (3) Gently put the 5th instar silkworm into the above spore suspension for 2 seconds, pick it up, put it into a sterilized petri dish with absorbent paper, and cultivate it for 3 weeks at room temperature with moisture (RH value above 80%) Get cicadas.

Embodiment 3

[0038] A kind of Cicada Isyphilis ( Isaria cicadae ) Qingcheng Mountain-16 cultivates the method for cicadae, comprises the following steps:

[0039] (1) (2) Synchronously with embodiment 2;

[0040] (3) Gently put the 5th instar silkworm into the above spore suspension for 3 seconds, then pick it up, put it into a sterilized petri dish with absorbent paper, keep it moist at 28°C (RH value above 80%) and cultivate for 3 Zhou got the cicada flower.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an Isariacicadae Qingcheng mountain-16 strain and use thereof. The collection number of the strain is CCTCCNO.m2010186. The mycological characteristics of the strain include: after the bacteria are cultured at 25+ / -1 DEG C in a Capek culture medium for 14 days, the diameter of the bacterial colony is 70 millimeters, the bacteria are panniform and flat and have a bean milk yellow powder layer, and the backside of the bacteria is colorless; and after the bacteria are cultured at 25+ / -1 DEG C in a Sabourd culture medium for 14 days, the diameter of the bacterial colony is 70 millimeters, the bacterial have a bean milk yellow centric powder wheel layer and have light tawny dews, and the backside of the bacteria is colorless. The strain can be used to obtain infected bodies which are the same as those obtained by CordycepsciecadaeShing collected in natural mountain forest.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of I. cicadae, and at the same time, the use of the strain of I. cicadae in inoculating silkworms to cultivate cicadas. Background technique [0002] Cicada is a complex of fungal parasitic nymphs and deadly worms and bacteria. It is named cicada because the spore bunches that grow out of its head are shaped like flower buds. Cicadae is an ancient, valuable and extremely valuable insect fungus. Cicada is a precious herbal medicine for clearing away heat, detoxifying, dispelling wind, analgesic and improving eyesight; Clinical trials have shown that cicadae can improve kidney function and slow down the complications of renal failure; cicadae can significantly increase the survival time of mice under normal pressure, hypoxia and high temperature, indicating that it has anti-stress and anti-fatigue effects, and has Good sedative and hypnotic effect. Cicadas generally grow in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14A01G1/04C12R1/645
Inventor 刘爱英邹晓胡海燕赵杰宏罗力
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products