Negundo chastetree fruit extract and application thereof

A technology of extract, Vitex chrysanthemum, applied in the field of medicine

A technology of extract, Vitex chrysanthemum, applied in the field of medicine

CN102258633BActive Publication Date: 2013-04-03SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
0 Cites 3 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Negundo chastetree fruit extract and application thereof
  • Negundo chastetree fruit extract and application thereof
  • Negundo chastetree fruit extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Preparation of Vitex Vitex Extract

[0019] After pulverizing the whole herb of Vitex Vitex, put it into an extraction tank, heat extract with 80% ethanol for 3 times, each time the amount of solvent is about 10 times of the amount of crude drug, each extraction time is 2 hours, combine the extracts and concentrate to dryness, and Further drying is obtained to obtain the total extract of Vitex Vitex, which is dissolved in water and applied to AB-8 macroporous adsorption resin, and the eluted part with 60-80% ethanol is collected, concentrated and dried to obtain the Vitex Vitex extract of the present invention.

Embodiment 2

[0020] Example 2: Anti-rheumatoid arthritis pharmacodynamic experiment of Vitex chrysanthemum extract

[0021] 2.1 Experimental materials

[0022] 24 adult male Wistar rats (provided by the Experimental Animal Center of the Second Military Medical University) of 180-220 g, ELISA kits for rat serum TNF-α and IL-1 (Sigma, USA). Main instruments: toe volume measuring instrument (YLS-7A, equipment station of Shandong Academy of Medical Sciences), microplate reader (BIO-RAD 550), centrifuge (Labofuge 400R, Heraeus company), electronic balance (FA 110A type, Shanghai Precision Scientific Instruments Ltd).

[0023] 2.2 Experimental modeling and administration

[0024] Before the experiment, 24 rats were weighed and randomly divided into 3 groups according to body weight, 8 rats in each group, free to eat and drink, and to drink water. Divided into three groups: blank group, model group, and Vitex japonica group. Blank group: without any treatment; model group: bovine type II collag...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of medicine. Rheumatoid arthritis (RA) is one of the most important diseases which are harmful to human health at present, and the formidable task and problem to be solved in the global medicine world is to seek high-efficiency and low-toxicity medicaments for preventing and treating the RA. The invention provides negundo chastetree fruit extract. A method for preparing the negundo chastetree fruit extract comprises the following steps of: performing hot extraction on negundo chastetree fruit by using hydrous ethanol, concentrating extracting solution, loading to macroporous adsorbent resin, eluting by using the hydrous ethanol, concentrating eluent and drying to obtain the negundo chastetree fruit extract. Pharmacodynamic study indicates that the negundo chastetree fruit extract can be used for preparing medicaments or food for resisting the rheumatoid arthritis. The negundo chastetree fruit extract is a new source for seeking the medicaments for resisting the rheumatoid arthritis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the extract of Vitex vetex and its application in medicine or food for preventing or treating rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (RA) is one of the most important diseases that endanger human health at present, and is called "the cancer that never dies". RA is a chronic systemic autoimmune inflammatory disease characterized by multi-joint involvement, synovial inflammation, and bone and cartilage destruction. Its pathogenesis has not yet been fully elucidated. Disorders are closely related (Imboden JB. The immunopathogenesis of rheumatoid arthritis. Annu Rev Pathol Mech Dis 2009.4: 417-434.). The prevalence of RA in my country is about 0.3%-0.4%, and there are about 4 million patients. The disability rate is 60% within 5-10 years of the disease course, and 90% of patients in the late stage lose their social labor force and self-care ability...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
03 Apr 2013
Publication
CN102258633B
IPC
A61K36/85; A61P19/02; A61P29/00
Inventors
郑承剑; 秦路平