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Trichlorfon biomimetic enzyme-linked immunosorbent assay method

A detection method, bionic immune technology, applied in measurement devices, color/spectral property measurement, instruments, etc.

Inactive Publication Date: 2011-12-21
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004]The purpose of the present invention is to overcome the defects of long detection time, expensive instrument, poor accuracy and cumbersome pre-treatment in the traditional trichlorfon detection method, and provide a A rapid detection method for trichlorfon

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  • Trichlorfon biomimetic enzyme-linked immunosorbent assay method
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  • Trichlorfon biomimetic enzyme-linked immunosorbent assay method

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Embodiment Construction

[0029] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0030] In the invention, the trichlorfon molecularly imprinted polymer film is used as a biomimetic antibody in conjunction with an enzyme-linked immunological technique, thereby establishing a rapid detection method with high sensitivity for the trichlorfon. Its specific embodiment is:

[0031] 1) Dissolve 1 mmol (257.45 mg) trichlorfon in 3 mL acetonitrile and 2 mL water, then add 2 mmol (86.09 mg) MAA, stir magnetically for 30 min, then add 2 mmol (198.2 mg) EGDMA and 0.03 g azobisiso Butyronitrile (AIBN), magnetically stirred for 1 h. Add 200 μL of the above mixed solution to each well of the 96-well ELISA plate for polymerization reaction for 18 hours, discard the reaction solution, and continuously elute with 1:9 (v / v) glacial acetic acid-methanol ...

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Abstract

The invention relates to a dipterex bionic enzyme linked immunosorbent detection method. The bionic enzyme linked immunosorbent detection method with high sensitivity on the dipterex is established by using a dipterex molecular imprinting polymer film as an artificial antibody instead of a biological antibody and by utilizing an enzyme linked immunosorbent assay principle. The lowest detection limit of the method is 7 ugL<-1> and is far less than the highest residue limit value; and the analysis time is greatly shortened (by 1.0 hour compared with the traditional biological immune analysis time), so the method is applicable to rapid detection of the dipterex. A bionic antibody prepared by the method can be reused for more than 50 times, so cost is greatly reduced; and the method is simplein pretreatment, high in sensitivity, simple in test operation and applicable to rapid detection of the dipterex in various kinds of food.

Description

technical field [0001] The invention relates to a bionic ELISA detection method for trichlorfon, which belongs to the technical field of food safety detection, in particular to a rapid and sensitive detection method for trichlorfon. Background technique [0002] Trichlorphon (trichlorphon, O, O-dimethyl-(2,2,2-trichloro-1-hydroxyethyl) phosphate) is an organophosphorus spectrum insecticide synthesized by Bayer in 1952 and start production. It has the characteristics of high efficiency, low toxicity, low residue, and good water solubility, so it is widely used in agriculture, forestry, horticulture, animal husbandry, and hygiene. Also because of its wide application and increasingly serious abuse, the damage, carcinogenicity and mutagenicity of trichlorfon to animal nervous system have attracted more and more attention. GB 16319-1996 stipulates that the maximum residue of trichlorfon in food shall not exceed 0.1 mg Kg -1 . [0003] At present, most domestic and foreign de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/31
Inventor 徐志祥孟玲乔旭光
Owner SHANDONG AGRICULTURAL UNIVERSITY
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