48 results about "Metrifonate" patented technology

The invention relates to a method for detecting ten types of organophosphorus pesticides by using magnetic separation-gas chromatography. The method is used for quickly detecting ten types of organophosphorus pesticides (dipterex, methamidophos, acephate, omethoate, moncrotophos, dimethoate, phosphamidon, parathion-methyl, malathion and chlorpyrifos) based on combination of solid-phase extraction, magnetic separation and the gas chromatography. The method comprises the following steps: preparing a magnetic solid-phase extraction agent, establishing a combination system of the magnetic separation and the gas chromatography, and detecting the organophosphorus pesticides in objects to be detected. A method capable of quickly separating and detecting ten types of organophosphorus pesticides at high sensitivity simultaneously is established by taking prepared magnetic chitosan microspheres with high absorption performance on the ten types of organophosphorus pesticides such as dipterex and the like as adsorption functional materials and using combination of the solid-phase extraction, magnetic separation and the gas chromatography. The method is simple in experimental operation, high in detection sensitivity, low in cost and applicable to detection of residues of ten types of organophosphorus pesticides such as trace dipterex and the like in all products.

The invention relates to a pharmaceutical composition based on an active ingredient that is enclosed in liposomes for topical, transdermal application. The interior of said liposomes comprises an acidic, aqueous medium containing at least one cholinesterase inhibitor, preferably from the group containing donepezil, rivastigmine, galantamine, physostigmine, heptylphysostigmine, phenserine, tolserine, cymserine, thiatolserine, thiacymserine, neostigmine, huperzine, tacrine, metrifonate and dichlorvos, or an enantiomer or derivative of at least one of said compounds. In addition, the invention relates to a method for producing said composition, optionally in a sterile form and also to the use of the liposomes charged with the active ingredient in various galenic formulations for topical, transdermal application with a depot effect in the epidermis, for the prophylaxis and / or treatment of cutaneous neuropathic pain or the loss of cutaneous sensory function as a result of neuropathy.

The invention relates to a dipterex bionic enzyme linked immunosorbent detection method. The bionic enzyme linked immunosorbent detection method with high sensitivity on the dipterex is established by using a dipterex molecular imprinting polymer film as an artificial antibody instead of a biological antibody and by utilizing an enzyme linked immunosorbent assay principle. The lowest detection limit of the method is 7 ugL<-1> and is far less than the highest residue limit value; and the analysis time is greatly shortened (by 1.0 hour compared with the traditional biological immune analysis time), so the method is applicable to rapid detection of the dipterex. A bionic antibody prepared by the method can be reused for more than 50 times, so cost is greatly reduced; and the method is simplein pretreatment, high in sensitivity, simple in test operation and applicable to rapid detection of the dipterex in various kinds of food.

The present invention discloses a pesticide main agent for controlling poplar longicorn and preparation made up by using said pesticide main agent. Said main agent is made up by mixing 2.0-20.0% of acetamiprid and 10.0-40.0% of dichlorvos or trichlorfon or acephate.

The invention discloses a liquid chromatography-tandem mass spectrometry method for measuring 18 semi-volatile organic pollutants in water. According to the method, a clean water sample such as surface water, drinking water, and the like is simply filtered by a 0.22 [mu]m micro-porous filter membrane, and then a liquid chromatography-mass spectrometry instrument is used to separate and detect the following 18 compounds in water: aniline, benzidine, acrylamide, picric acid, carbaryl, deltamethrin, microcystic toxin-LR, dibutyl phthalate, bis(2-ethylhexyl) phthalate, dichlorvos, trichlorphon, parathion, methyl parathion, malathion, dimethoate, demeton, atrazine, and carbofuran. The provided method can detect many target substances. The detection range of the method is 0.001 to 0.49 [mu]g / L, the relative standard deviation is 0.7 to 15.4%; and the analysis method has the advantages of high sensitivity, quick analysis speed, little pollution, and simple and efficient pretreatment, is especially suitable for standard analysis of drinking water source monitoring, and solves the problems of bad conformability, large labor strength, and low analysis efficiency of the conventional standard method.

The invention belongs to the technical field of content determination of trichlorfon, and provides a content determining method for trichlorfon in dried fish. The method comprises the following specific steps of: (1) preparing a standard curve; (2) preparing a sample solution, to be specific, (1) pretreating; (2) extracting; (3) concentrating; and (4) purifying; and (3) detecting the sample with a gas chromatography-mass spectrometry method. Compared with the prior art, the method has the advantages that: during preparation of the sample solution, a purifying step in a pretreatment process is mainly increased, so that the interference of greasy substances in the sample on determination is greatly lowered, and the probability of a false positive result is lowered; and trichlorfon is detected by using the gas chromatography-mass spectrometry method, so that determination is accurate, and the detection sensitivity is high.

The invention discloses a cultivation method for peony plantation which comprises following steps: land selection: high-lying sunny lands with total salt content under 0.3%, good water drainage and loose soil are selected; seed selection: full seeds selected out by wet cleaning process are soaked in warm water of 50 DEG C; seedling growing: sowing is carried out in September to October and drill sowing is carried out with wet plant ash after mixing; transplanting: over-thin and overlong roots are trimmed so that the length of roots is 20 cm, then the roots are soaked in mass fraction 50% Thiram 800 times solution for 15 to 20 minutes, and then dried and planted; field management: weeding is performed 3 to 4 times and additional fertilizer is applied 2 times every year; insect disease controlling: according to the types of plant diseases, 1:1:120 Bordeaux mixture and 0.3 to 0.4 DEG calcium polysulphides are reasonably sprayed, and 1:800 trichlorfon is used for controlling insect pest. By means of the method of the invention, peony grows fast and has high survival rate, good economic benefit and social benefit; the method is suitable for promotion.

The invention relates to the technical field of medicine for prevention and control of diseases and insect pests, and especially relates to a citrus tetradacus citri prevention and control liquid anda preparation method thereof; brown sugar, Baijiu, white vinegar, trichlorfon and water are controlled according to a certain proportion, the trapping effect of the insect prevention liquid is significantly enhanced, the effect of prevention and control of citrus tetradacus citri is improved, and the citrus planting cost is reduced.

The invention relates to the preparing method of soybean exterase which can be used in the detection of the vegetable and fruits' pesticide residue. The producing procedure comprises the following steps: choosing the high-quality soybean, pulverizing, extracting with phosphate buffer, differential centrifugation separating, salt precipitating by ammonium, purifying by ion exchange chromatography and freeze-drying. The invention has a simple and steady producing technology. It also costs less than the method of extracting pure acetylcholine exterase products. The purified product's sensitivity is more than 19 times as the sensitivity of the enzyme crude extracts. The LDC of monocrotophos, methamidophos, alkron, durshan, dantox, carbofos and chlorofos has reached 0.03125ppm. The LDC of dursan and methidathion is one thirty-second of their MRL, while the LDC of diethion, alkron and Phosalone is one sixteenth of their MRL. The invention can fill the needs of fast detection of phosphate pesticide; it can also be widely used as the enzyme labeling marker in different methods of detecting the pesticide residue.

The invention relates to a Rhodobacter sphaeroides and an application thereof. A novel strain Rhodobacter sphaeroides XR12 screened by the inventors has high tolerance to trichlorfon in a water environment or an aquaculture environment, can reduce the toxicity of trichlorfon in the water environment or the aquaculture environment, and can be applied to the aquaculture field or the water environment treatment field.

An antibactieral composite fertilizer contains N and / or P and / or K fertilizer (10-90 wt.%), naphthalene (0.5-3), chlorothalonil (0.01-1), trichlorfon (0.05-1.2), phoxim (0.01-1.1) and auxiliary. Its advantages are rich nutrients, high insecticiding effect and increasing yield of crops.

The pesticide for crop has effective components including trichlorfon and high effect cypermethrin or cypermethrin in the weight ratio of 1-130, preferably 25-45 and supplementary components including surfactant, agglomeration resisting agent and filler. It is diluted with water before sprayed to rice field to prevent and kill striped rice borer and yellow rice borer and other borer with the use amount being 50-150 g each mou.

The invention discloses a plastic greenhouse soilless flowing water culture technical scheme for eels and belongs to the technical field of aquaculture. Rearing ponds are built by adopting bricks and cement, the area of each pond is 10-20 square meters, each pond is 40cm deep and 1-2m wide, and a pond bank is 20-40cm wide; a plurality of rearing ponds are arranged in parallel to form one unit, the area of each unit is best not more than 500 square meters; and 0.1g of 90% crystal trichlorfon is used for per kilogram of eels and is mixed into feed for feeding for six days continuously, so that the technical scheme is completely implemented. The technical scheme disclosed by the invention is beneficial to improving cultivation quality of the eels, health is important due to edible eels, disinfection treatment in each aspect and control methods of plant diseases and insect pests are beneficial to improving health condition of the eels, self physique is perfect, and great taste when the eels are eaten can be enhanced, so that the technical scheme disclosed by the invention has better economic value and is convenient for popularization.

The invention belongs to the technical field of pesticides and particularly relates to a special mite-killing chemical for bees and a use method. According to the technical scheme, the special mite-killing chemical for the bees comprises components in percentage by weight as follows: 15%-25% of sublimed sulfur, 10%-20% of carbolic acid, 5%-15% of trichlorfon, 10%-20% of boric acid, 15%-25% of boric acid, 5%-15% of naphthalene and the balance of coal ash, and the components are mixed to prepare powder. The chemical contains simple components and does not contain chemical drugs that are harmful to a human body, the components are easy to volatilize and decompose and are pollution-free for bee products, and the chemical is volatilized quickly, is safe and harmless for the bees and has high mite-killing efficiency.

The invention discloses a preparation method and applications of a cDNA (complementary Deoxyribonucleic Acid) chip for detecting drug resistance level of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin. Upregulated drug resistance specificity expression gene segments of different resistant strains of bactrocera dorsalis can be obtained respectively by utilizing a forward suppression subtractive hybridization method, the related drug-resistant specificity expression genes are subjected to PCR (Polymerase Chain Reaction) amplification, 118 related resistance specificity gene segments of bactrocera dorsalis resistant to trichlorphon and 103 related resistance specificity gene segments of bactrocera dorsalis resistant to beta-cypermethrin can be obtained through purifying and recovering. The cDNA chip for detecting the resistance of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin can be constructed by taking the specific resistant target gene segments as DNA probes. The cDNA chip can simply and effectively realize the real-time detection on the drug resistance level of bactrocera dorsalis.

The invention relates to an ultra low-volume liquid containing metaflumizone and an organophosphorus pesticide. The ultra low-volume liquid comprises a chemical component compounded of metaflumizone and an active ingredient II, and the balance at least one surfactant and at least one solvent, wherein the active ingredient II is any one of fenitrothion, trichlorfon, methyl parathion, isazofos, pyridafenthion, quinalphos, triazophos, fosthiazate, diazinon, phoxim, fenitrothion, pirimiphos-methyl, fenthion, cyanophos, profenofos, chlorpyrifos, chlorpyrifos-methyl, prothiofos, sulprofos, dimethoate, malathion, methidathion and isocarbophos. The ultra low-volume liquid is convenient for processing, has a long duration of efficacy and high efficacy, does not cause pesticide resistance of targets easily, produces low pollution on the environment, saves pesticides and water, improves a pesticide utilization rate, and realizes control of insect pests damaging paddy, wheat, corn, vegetables, tobacco, tea trees, fruit trees and sugarcane through ultra low-volume spraying, low-volume spraying or ultra low-volume electrostatic spraying.

An antibactieral composite fertilizer contains N and / or P and / or K fertilizer (10-90 wt.%), naphthalene (0.5-3), chlorothalonil (0.01-1), trichlorfon (0.05-1.2), phoxim (0.01-1.1) and auxiliary. Its advantages are rich nutrients, high insecticiding effect and increasing yield of crops.

The present invention relates to synergistic precursor medicine compound for treating Alzheimer disease or its salt and their preparation process, and medicine composition containing the compound or its salt and their application in treating Alzheimer disease. Specially, the compound, which is (1-dimethyl phosphoryl-2, 2, 2-trichloro)-ethyl-1-alcohol nicotinate, is formed with nicotinic acid and metrifonate and through ester bond coupling. The compound or its salt of the present invention has excellent effect on treating Alzheimer disease.

The invention relates to continuous granulation process equipment for trichlorfon. Synthesized trichlorfon materials are put into a heat preservation kettle to be gradually cooled, it is guaranteed that the materials are in a liquid state, and then the materials are conveyed into a pre-crystallizer through a feeding pump to be slightly cooled; and trichlorfon slurry flowing out of the pre-crystallizer is fed into a granulator. The materials are dropped on an annular steel belt through a distributing device, a cooling liquid spraying device can be arranged between the distributing device and the annular steel belt, trichlorfon liquid drops are dropped in cooling liquid to form hard spherical white particles, and then the hard spherical white particles flow out, and finally the hard spherical white particles are dried and cooled. According to the equipment of the invention, no three wastes are discharged basically, so that occupational health injury is reduced; heat generated by synthesis of the trichlorfon is recycled through heat exchange, so that environmental protection and energy conservation are realized; and the particle size of the finished product is more uniform and rounded, and the appearance and the production efficiency of the product are improved.

The invention discloses a preparation method of a trichlorfon sol-gel imprinted sensor, which sequentially comprises the following steps: pretreating a glassy carbon electrode, reducing a graphene oxide modified electrode, preparing a sol-gel pre-polymerization solution, preparing a sol-gel imprinted membrane by electro-polymerization, eluting template molecules and the like. According to the invention, the trichlorfon is used as a template molecule, methyltrimethoxysilane is used as a functional monomer and tetramethoxysilane is used as a cross-linking agent; the imprinted polymer membrane prepared by using a sol-gel method by is high in rigidity and relatively large in specific surface area, so that template molecules and the functional monomer can form a stable host-guest compound; the template molecules can keep a good rigid structure in a polymer net structure and form a cavity with a memory effect, and the prepared sensor is high in selectivity, good in stability, high in sensitivity, low in cost and fast in response. The method is suitable for rapidly detecting the trichlorfon residues in the plant-derived food.

The invention, which relates to the technical field of food detection, discloses a detection method for measuring the trichlorfon in meat food by liquid chromatography-tandem mass spectrometry. The method comprises: 2g to 10g of pork samples are weighed by using an electronic scale, the pork samples are placed in a beaker, 30mL to 100mL of ethyl acetate is added into the beaker, the samples and the ethyl acetate are mixed uniformly by using a homogenizer, and then ultrasonic processing is carried out for 10 min to 20 min in normal temperature water bath; the ethyl acetate extract of the pork samples is transferred to a separating funnel, centrifugal processing is carried out for 5 to 10min in a centrifuge tube at a centrifugal speed that is controlled to be 8000-10000r / min, so that an extract is obtained. According to the invention, the trichlorfon in the port is detected by using the liquid chromatography tandem mass spectrometry and the separation ability of chromatography is combined with the qualitative function of mass spectrometry, thereby realizing the accurate quantitative and qualitative analysis of trichlorfon in pork food. In detection of the trichlorfon residues in porkfood, the interference can be effectively eliminated and the stability of a to-be-tested object can be ensured. Moreover, the detection and analysis method has high sensitivity and high accuracy.

The invention provides a preparation method and production equipment for continuous production of trichlorfon, and effectively solves the problems of low production efficiency, poor production stability and safety, poor product quality and low yield of the existing production method. The continuous production method of trichlorfon comprises the following two stages of synthesis reactions for continuously preparing trichlorfon: (1) adding trichlorfon into a I-stage synthesis kettle as a synthesis kettle reaction mother liquor, then simultaneously dropwise adding trichloroacetaldehyde and dimethyl phosphite for reaction, and controlling the reaction temperature to be 75-85 DEG C; (2) enabling the reaction materials to enter a second-stage synthesis kettle from the first-stage synthesis kettle to continuously react, and controlling the reaction temperature to be 75 DEG C; and the product trichlorfon is obtained after the reaction in the I-stage synthesis kettle and the II-stage synthesiskettle.

The invention discloses a pesticide residue degradation nutrient solution which comprises the following substance components in percentage: 37% of fertilizer, 4% of bacillus subtilis, 3% of white rot fungi, 5% of aspergillus, 4% of photosynthetic bacteria and 45% of underground water. The invention also discloses a preparation method of the pesticide residue degradation nutrient solution. The preparation method comprises the following steps: S1, calculating the weight of the required fertilizer, bacillus subtilis, white rot fungi, aspergillus, photosynthetic bacteria and underground water, and weighing for later use; s2, placing the underground water in a reaction kettle, heating the underground water in the reaction kettle, and keeping the underground water at a constant temperature; and S3, crushing the fertilizer. Fertilizer of the pesticide residue degradation nutrient solution can provide nutritional ingredients for crops and is beneficial to growth of the crops, meanwhile, bacillus subtilis, white rot fungi, aspergillus and photosynthetic bacteria are added into the nutrient solution, pesticides such as trichlorfon and prometryn can be degraded, and guarantee is provided for health of people.

The invention discloses a liquid chromatography-tandem mass spectrometry method for measuring 18 semi-volatile organic pollutants in water. According to the method, a clean water sample such as surface water, drinking water, and the like is simply filtered by a 0.22 [mu]m micro-porous filter membrane, and then a liquid chromatography-mass spectrometry instrument is used to separate and detect the following 18 compounds in water: aniline, benzidine, acrylamide, picric acid, carbaryl, deltamethrin, microcystic toxin-LR, dibutyl phthalate, bis(2-ethylhexyl) phthalate, dichlorvos, trichlorphon, parathion, methyl parathion, malathion, dimethoate, demeton, atrazine, and carbofuran. The provided method can detect many target substances. The detection range of the method is 0.001 to 0.49 [mu]g / L, the relative standard deviation is 0.7 to 15.4%; and the analysis method has the advantages of high sensitivity, quick analysis speed, little pollution, and simple and efficient pretreatment, is especially suitable for standard analysis of drinking water source monitoring, and solves the problems of bad conformability, large labor strength, and low analysis efficiency of the conventional standard method.

The invention discloses a cDNA chip for detecting the resistance level of Bactrocera dorsalis against trichlorfon and beta-cypermethrin, a production method and application thereof. The present invention utilizes the forward suppressive subtractive hybridization (suppression subtractive hybridization, SSH) method to respectively obtain different resistant strains of Bacteralis dorsalis drug resistance-specific up-regulated expression gene fragments, and then PCR amplifies each drug resistance-related specific expression gene, purifies and recovers 118 specific gene fragments related to resistance to trichlorfon of Bactrocera dorsalis and 103 specific gene fragments related to resistance to beta-cypermethrin of Bactrocera dorsalis were obtained. Using the above-mentioned specific resistance target gene fragments as DNA probes, a cDNA chip for the detection of Bacteralis dorsalis resistance to trichlorfon and beta-cypermethrin was constructed. The cDNA chip of the invention can simply and effectively realize the real-time monitoring of the drug resistance level of Bactrocera dorsalis.