Preparation method and applications of cDNA chip for detecting drug resistance level of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin

A technology of high-efficacy cypermethrin and Bactrocera dorsalis, applied in biochemical equipment and methods, measurement/testing of microorganisms, chemical library, etc., can solve the problems of lack of reports, achieve simple and effective process, delay drug resistance, and realize food production safe effect

Active Publication Date: 2014-03-05
SOUTH CHINA AGRI UNIV
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few reports on how many genes are involved in drug resistance, whether different drug resistan...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and applications of cDNA chip for detecting drug resistance level of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin
  • Preparation method and applications of cDNA chip for detecting drug resistance level of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin
  • Preparation method and applications of cDNA chip for detecting drug resistance level of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Test the construction of CDNA chips with anti -enemy cachines and high -efficiency cylinoblobroblobrotlobal chlorophytte

[0035] 1. The construction of the SSH Library of the Orange Small Fly Anti -Enemy and Effective Berbin Elear

[0036] (1) Selection of Orange Xiaolishi Flies Resistance Products:

[0037] The sensitive product reserved with a laboratory is used as a pro -agency F for the screening of the anti -drug product system 0 It is raised under the same conditions of indoor, and the pharmaceutical film method is used to scree with enemy cachines and high -efficiency chloride.

[0038] Sensitive product F 0 Standard for selection: The concentration of the death of the enemy's sensitive products of the enemy's small solid fly adults LC 50 = 2.65mg / L; the concentration of high -efficiency cyanoline to the death of the sensitive product of the orange flies 50 = 3.36mg / L.

[0039] Obtained by the Department of Resistance: After the screening of some algebrabi...

Embodiment 2

[0054] Example 2 The CDNA chip prepared by the present invention to detect the anti -drug resistance of the orange flies

[0055] 1. Selection of Orange Xiaoshi Fly Anti -sex products

[0056] The sensitive products reserved with a laboratory are used as the pro -agent F0 of the anti -drug product system. They are raised under the same conditions indoors. The pharmaceutical film method is used to scree with enemy cachines and high -efficiency chloride.Specific steps are the same.The results obtained 3.21 times, 18.23 times, and 69.5 times the anti -aggressive anti -enemy antidotians; 6.8 times, 16.2 times, and 160.74 times of anti -highly semonal chloride.At the same time, 160.74 times the antidine chloride ester is determined that the interactive resistance to enemy cachines is 21.20 times.

[0057] 2. Orange Xiaoshi Fly Anti -sex products and sensitive product sample total RNA extraction

[0058] Each anti -sex product and sensitive product of the selected breeding are extracted...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method and applications of a cDNA (complementary Deoxyribonucleic Acid) chip for detecting drug resistance level of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin. Upregulated drug resistance specificity expression gene segments of different resistant strains of bactrocera dorsalis can be obtained respectively by utilizing a forward suppression subtractive hybridization method, the related drug-resistant specificity expression genes are subjected to PCR (Polymerase Chain Reaction) amplification, 118 related resistance specificity gene segments of bactrocera dorsalis resistant to trichlorphon and 103 related resistance specificity gene segments of bactrocera dorsalis resistant to beta-cypermethrin can be obtained through purifying and recovering. The cDNA chip for detecting the resistance of bactrocera dorsalis resistant to trichlorphon and beta-cypermethrin can be constructed by taking the specific resistant target gene segments as DNA probes. The cDNA chip can simply and effectively realize the real-time detection on the drug resistance level of bactrocera dorsalis.

Description

Technical field [0001] The invention involves the field of insect resistance testing technology.More specifically, a CDNA chip and its preparation methods and applications involving a CDNA chip and its preparation methods and applications that detect the anti -enemy cachines and high -efficiency chloroline. Background technique [0002] The World Health Organization's definition of insect resistance in 1957 is: "insects have the ability to resist the weight of most individuals in the normal population and develop in their population."The resistance of insects for insecticides is an evolutionary phenomenon. It is an external manifestation of continuous changes in the internal genetic structure of insect populations under the effect of pesticides.Drug resistance, negative interoperability resistance and multi -drug resistance.The consequences of insects produce anti-medicinal properties are: the current effect of the prevention and treatment of chemical chemicals is reduced-people ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C40B40/08C40B50/06
CPCC12Q1/6888C12Q2600/158
Inventor 曾玲姜建军陆永跃何晓芳梁广文温硕洋
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap