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Composition for alleviating ultraviolet radiation-induced damage

By using a composition of free methionine and its derivatives, the problem of instability and unsafety of ultraviolet damage preventive agents in the prior art is solved, and a stable and safe ultraviolet damage reduction method is provided, suitable for daily use and treatment of ultraviolet rays Caused skin diseases and cataracts.

Inactive Publication Date: 2014-04-16
SHISEIDO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since UV-A is not absorbed by the ozone layer, UV-A accounts for most of the ultraviolet rays reaching the ground, causing skin damage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] UV damage reduction effect of methionine

[0062] method

[0063] cell culture

[0064] As the cells, commercially available human neonatal dermal fibroblasts (Cryo NHDF-Neo, Sanko Junyaku) were used. The cells according to 2×10 5Cells / mL were inoculated in a commercially available 35mm diameter Petri dish (BD FALCON 353001, Becton Dickinson, Japan), using a commercially available cell culture medium (D-MEM (1g / L glucose), Wako Pure Medicine) supplemented with 10% fetal bovine serum (hereinafter referred to as "common medium") for culture. The cells can be cultured in a medium supplemented with 1% antibiotic (15240-062, GIBCO) in the normal medium. The cells were incubated at 37°C, 5% CO 2 and cultivated under saturated water vapor conditions for about 24 hours.

[0065] Afterwards, the medium used for culturing the cells was replaced with 1×10 -3 % BSO medium 1 mL of BSO (L-buthionine-(S, R)-sulfoximine, Wako Pure Chemical Industries, Ltd.) as a biosynthesis inh...

Embodiment 2

[0086] UV damage reduction effect of serine

[0087] method

[0088] Cell culture, amino acid addition before ultraviolet irradiation, ultraviolet irradiation, amino acid addition after ultraviolet irradiation, and quantification of cell damage were performed in the same manner as in Example 1. With 7.5 or 9J / cm 2 Irradiate with ultraviolet rays (UV-A). When studying the effect of adding serine before ultraviolet irradiation (hereinafter referred to as "serine before irradiation") and adding serine after ultraviolet irradiation (hereinafter referred to as "serine after irradiation"), 0.0001 to 100 μM of L-serine (197 -00403, Wako Pure Chemicals) and D-serine (197-08823, Wako Pure Chemicals). The effect of adding serine after irradiation was evaluated by irradiating cells with 7.5 J / cm 2 After UV-A, return to normal culture medium for 21 hours, and add D-serine to the 21-hour culture medium for evaluation.

[0089] Results of adding serine before irradiation (1)

[0090] ...

Embodiment 3

[0099] UV damage reduction effect of D-cycloserine

[0100] method

[0101] Cell culture, amino acid addition before ultraviolet irradiation, ultraviolet irradiation, amino acid addition after ultraviolet irradiation, and quantification of cell damage were performed in the same manner as in Example 1. at 9J / cm 2 Irradiate with ultraviolet rays (UV-A). When examining the effect of adding D-cycloserine before ultraviolet irradiation (hereinafter referred to as "addition of D-cycloserine before irradiation"), 0.0001 to 100 μM of D-cycloserine (C6880, Sigma) was used.

[0102] Result of adding D-cycloserine before irradiation

[0103] Figure 7 shows the effect of investigating the effect of adding D-cycloserine on irradiation by UV-A9J / cm 2 Experimental results of the damage effect of ultraviolet rays on fibroblasts. The error bar of each experimental condition represents the standard deviation of the measured value of the experimental result repeated 3 to 4 times under the...

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PUM

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Abstract

Disclosed are a composition for alleviating ultraviolet radiation-induced damage, which is stable and safe and can be used on a day-to-day basis, and a medicinal composition, an external skin preparation, a food composition and a medicine for cataract, each comprising said composition for alleviating ultraviolet radiation-induced damage. Specifically disclosed is a composition for alleviating ultraviolet radiation-induced damage which contains one or more kinds of compounds selected from the group consisting of methionine, D-serine and derivatives and / or salts of the same. The aforesaid composition may be an external skin preparation, an anti-wrinkle agent, a sunscreen agent, a medicinal composition for treating and / or preventing skin diseases, a food composition or a medicine for cataract. In the aforesaid composition for alleviating ultraviolet radiation-induced damage, said methionine may be D-methionine, and said D-serine derivative may be D-cycloserine.

Description

technical field [0001] The present invention relates to an ultraviolet damage reducing composition, which contains one or more compounds selected from the group consisting of methionine, D-serine and their derivatives and / or salts; Caused skin disease and method for improving the cosmetic state of skin, the method comprising the step of administering one or more compounds selected from the group consisting of methionine, D-serine, and their derivatives and / or salts . Background technique [0002] Ultraviolet rays can be divided into long-wavelength ultraviolet rays (UV-A) longer than about 320nm, middle-wavelength ultraviolet rays (UV-B) from about 320nm to about 280nm, and short-wavelength ultraviolet rays (UV-C) shorter than about 280nm. Among them, since UV-C is absorbed by the ozone layer, the sunlight reaching the ground does not contain UV-C. Both UV-A and UV-B are contained in sunlight reaching the ground, but UV-B is partially absorbed by the ozone layer. However,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/198A23L1/305A61K8/44A61P17/00A61P17/16A61P35/00A61P37/02A61P37/08A61Q17/04
CPCA61K9/0014A61K9/12A61K9/7007A61K9/4858A61K8/44A61Q19/004A61K8/0208A61K9/107A23V2002/00A61K31/198A61K9/2018A61Q17/04A61Q19/08A23L1/3051A23L33/175A61P17/00A61P17/16A61P27/12A61P35/00A61P37/02A61P37/08A23V2200/318A23V2250/0632A23V2250/0642
Owner SHISEIDO CO LTD
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