Method for preparing antibacterial compound from scorpion
The technology of a compound and whole scorpion is applied in the field of preparation of antibacterial compounds and achieves the effects of simple operation process, broad-spectrum antibacterial effect and obvious antibacterial effect.
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Embodiment 1
[0048] The method for preparing antibacterial compound from scorpion, concrete steps are:
[0049] Step 1, add 500g of scorpion into 700ml of dichloromethane, soak for 2 days, filter to obtain filter residue, set aside;
[0050] Step 2, according to the method of step 1, repeatedly soak and filter the filter residue twice to obtain the filter residue, dry it, and set aside;
[0051] Step 3. Add 12 g of the dried filter residue in step 2 to 700 ml of methanol with a concentration of 60%, soak for 2 days, and filter to obtain filtrate I and filter residue for subsequent use;
[0052] Step 4, according to the method of step 3, repeatedly soak and filter the filter residue in step 3 twice, collect the filtrate after soaking and filtering each time, mix the filtrate I and the filtrate collected each time to obtain a mixed solution, spare;
[0053] Step 5. Place the mixed solution in step 4 at -60°C, freeze until it becomes a paste mixture, send the frozen paste mixture into a fre...
Embodiment 2
[0060] The method for preparing antibacterial compound from scorpion, its concrete steps are:
[0061] Step 1, add 500g of scorpion into 850ml of dichloromethane, soak for 3 days, filter to obtain filter residue, set aside;
[0062] Step 2, according to the method of step 1, repeatedly soak and filter the filter residue 3 times to obtain the filter residue, dry it, and set aside;
[0063] Step 3. Add 18 g of the dried filter residue in step 2 to 850 ml of methanol with a concentration of 70%, soak for 3 days, and filter to obtain filtrate I and filter residue for subsequent use;
[0064] Step 4, according to the method of step 3, repeatedly soak and filter the filter residue in step 3 for 3 times, collect the filtrate after soaking and filtering each time, mix the filtrate I and the filtrate collected each time to obtain a mixed solution, spare;
[0065] Step 5. Place the mixed solution in step 4 at -60°C, freeze until it becomes a paste mixture, send the frozen paste mixtur...
Embodiment 3
[0072] The method for preparing antibacterial compound from scorpion, its concrete steps are:
[0073] Step 1, add 500g of scorpion into 1000ml of dichloromethane, soak for 4 days, filter to obtain filter residue, set aside;
[0074] Step 2, according to the method of step 1, repeatedly soak and filter the filter residue 3 times to obtain the filter residue, dry it, and set aside;
[0075] Step 3, add 24g of the dried filter residue in step 2 to 1000ml of methanol with a concentration of 80%, soak for 4 days, filter to obtain filtrate I and filter residue, and set aside;
[0076] Step 4, according to the method of step 3, repeatedly soak and filter the filter residue in step 3 4 times, collect the filtrate after soaking and filtering each time, mix the filtrate I and the filtrate collected each time to obtain a mixed solution, spare;
[0077] Step 5. Place the mixed solution in step 4 at -60°C, freeze until it becomes a paste mixture, send the frozen paste mixture into a fre...
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