Method for preparing antibacterial compound from scorpion

The technology of a compound and whole scorpion is applied in the field of preparation of antibacterial compounds and achieves the effects of simple operation process, broad-spectrum antibacterial effect and obvious antibacterial effect.

Inactive Publication Date: 2014-04-02
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] With the development of science and technology at home and abroad, the research on scorpions is deepening day by day, but most of them focus on the research of scorpion venom. The active ingredients of scorpion venom are mainly polypeptides, and the research on the small molecule antibacterial compounds of secondary metabolites is still in progress. No relevant reports

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  • Method for preparing antibacterial compound from scorpion
  • Method for preparing antibacterial compound from scorpion
  • Method for preparing antibacterial compound from scorpion

Examples

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Effect test

Embodiment 1

[0048] The method for preparing antibacterial compound from scorpion, concrete steps are:

[0049] Step 1, add 500g of scorpion into 700ml of dichloromethane, soak for 2 days, filter to obtain filter residue, set aside;

[0050] Step 2, according to the method of step 1, repeatedly soak and filter the filter residue twice to obtain the filter residue, dry it, and set aside;

[0051] Step 3. Add 12 g of the dried filter residue in step 2 to 700 ml of methanol with a concentration of 60%, soak for 2 days, and filter to obtain filtrate I and filter residue for subsequent use;

[0052] Step 4, according to the method of step 3, repeatedly soak and filter the filter residue in step 3 twice, collect the filtrate after soaking and filtering each time, mix the filtrate I and the filtrate collected each time to obtain a mixed solution, spare;

[0053] Step 5. Place the mixed solution in step 4 at -60°C, freeze until it becomes a paste mixture, send the frozen paste mixture into a fre...

Embodiment 2

[0060] The method for preparing antibacterial compound from scorpion, its concrete steps are:

[0061] Step 1, add 500g of scorpion into 850ml of dichloromethane, soak for 3 days, filter to obtain filter residue, set aside;

[0062] Step 2, according to the method of step 1, repeatedly soak and filter the filter residue 3 times to obtain the filter residue, dry it, and set aside;

[0063] Step 3. Add 18 g of the dried filter residue in step 2 to 850 ml of methanol with a concentration of 70%, soak for 3 days, and filter to obtain filtrate I and filter residue for subsequent use;

[0064] Step 4, according to the method of step 3, repeatedly soak and filter the filter residue in step 3 for 3 times, collect the filtrate after soaking and filtering each time, mix the filtrate I and the filtrate collected each time to obtain a mixed solution, spare;

[0065] Step 5. Place the mixed solution in step 4 at -60°C, freeze until it becomes a paste mixture, send the frozen paste mixtur...

Embodiment 3

[0072] The method for preparing antibacterial compound from scorpion, its concrete steps are:

[0073] Step 1, add 500g of scorpion into 1000ml of dichloromethane, soak for 4 days, filter to obtain filter residue, set aside;

[0074] Step 2, according to the method of step 1, repeatedly soak and filter the filter residue 3 times to obtain the filter residue, dry it, and set aside;

[0075] Step 3, add 24g of the dried filter residue in step 2 to 1000ml of methanol with a concentration of 80%, soak for 4 days, filter to obtain filtrate I and filter residue, and set aside;

[0076] Step 4, according to the method of step 3, repeatedly soak and filter the filter residue in step 3 4 times, collect the filtrate after soaking and filtering each time, mix the filtrate I and the filtrate collected each time to obtain a mixed solution, spare;

[0077] Step 5. Place the mixed solution in step 4 at -60°C, freeze until it becomes a paste mixture, send the frozen paste mixture into a fre...

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Abstract

The invention relates to a method for preparing an antibacterial compound from a scorpion. The method comprises: adding a scorpion into dichloromethane for immersing and filtering so as to obtain a filter residue, which is then added into methanol for immersing and filtering, thus obtaining a filter solution I and a filter residue, then conducting immersion and filtration to the filter residue for 2-4 times, collecting the filter solution of each time, and mixing the filter solution I with the filter solutions collected each time so as to obtain a mixed solution, freezing the mixed solution at a temperature of -60DEG C to a paste mixture, then drying the mixture at a temperature of -54DEG C so as to obtain a crude extract, then preparing the crude extract and methanol with a concentration of 70% into a methanol extract containing the crude extract and having a concentration of 60mg / ml, carrying out gradient elution and separation to the methanol extract by a high performance liquid chromatography analytical method, thus obtaining fractions with biological activity, mixing the fractions having biological activity with a silica gel uniformly to form a loading solution, which is subjected to elution and separation through a silica gel column chromatography method, thus obtaining a product I and a product II. The preparation method of the invention is characterized by simple operation, and the antibacterial compound from a scorpion has an obvious antibacterial effect.

Description

technical field [0001] The invention relates to a method for preparing an antibacterial compound, in particular to a method for preparing an antibacterial compound from a scorpion. Background technique [0002] Arthropods, also known as "arthropods", are the most diverse phylum in the animal kingdom, accounting for 85% of the known animals, reaching more than 1 million species, and the number of each species is astonishingly large, almost in any space on the earth. Arthropods can be found. Common ones include centipedes, scorpions, spiders and various insects. Traditional Chinese medicine is a complex material system with rich scientific connotations. However, the complexity of the material components and activities of traditional Chinese medicine is beyond imagination. In the current era of rapid technological development, the material basis and mechanism of action of herbal medicines are still a major scientific problem facing mankind. As a traditional animal Chinese me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J19/00C07D239/06A61K31/585A61K31/505A61P31/04
Inventor 尹卫平高婷刘普任云利邓瑞雪
Owner HENAN UNIV OF SCI & TECH
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