Polarization interference multi-element CARS (Coherent Anti-stokes Raman Scattering) microscopic imaging method

Abstract

The invention relates to a polarization interference multi-element CARS (Coherent Anti-stokes Raman Scattering) microscopic imaging method. The method comprises the steps that: high and heavy frequency polarization femtosecond laser is taken as a probe light of a CARS signal, and a part of the high and heavy frequency polarization femtosecond laser after beam splitting is taken as pump light pump PCF (Photon Crystal Fiber); and the output of a super continuous spectrum generated by the PCF is divided into two parts, a part is taken as a first Stokes exciting light, the other part is taken as a second Stokes exciting light after the polarization angle of the super continuous spectrum is changed by a half wave plate, and a CARS microscopic image is obtained through a confocal microscopic optical path and a spectrum analysis system. The polarization interference multi-element CARS microscopic imaging method provided by the invention has the advantages that: femtosecond CARS probe light and two beams of the super continuous spectrum Stokes exciting lights generated by the pump PCF after the beam splitting are subjected to polarization interference, and polarized component in some one direction is extracted and a peak-to-peak value after interference enhancement is selected through a polarization analyzer, thus the influence of off-resonance background noise of a sample can be eliminated, and the CARS microscopic image with high contrast and broad band is obtained.

Description

technical field [0001] The invention relates to a polarization interference multivariate CARS microscopic imaging method. Background technique [0002] Coherent anti-Stokes Raman scattering (CARS) is a third-order nonlinear optical process. CARS microscopic imaging technology can obtain the spatial distribution images of specific molecules with high sensitivity, as well as the The dynamic process of the interaction between them is monitored. Traditional CARS microscopic imaging technology uses narrow-linewidth picosecond laser pulses to obtain CARS images of specific chemical bonds, but for the study of molecules with complex components and structures, it is necessary to adjust the laser point by point It is time-consuming and difficult to use in order to obtain the complete CARS spectral information of the molecule at the center wavelength of the molecule. In addition, the quality of CARS microscopic imaging is related to the background of the sample, and the solvent and ot...

AI Technical Summary

Problems solved by technology

[0002] Coherent anti-Stokes Raman scattering (CARS) is a third-order nonlinear optical process. CARS microscopic imaging technology can obtain the spatial distribution images of specific molecules with high sensitivity, as well as the The dynamic process of the interaction between them is monitored. Traditional CARS microscopic imaging technology uses narrow-linewidth picosecond laser pulses to obtain CARS images of specific chemical bonds, but for the study of molecules with complex components and structures, it is necessary to adjust the laser point by point It is time-consuming and difficult to use in order to obtain the complete CARS spectral information of the molecule at the center wavelength of the molecule. In addition, the quality of CARS microscopic imaging is related to the background of the sample, and the solvent and other media around the biological sample will produce strong non-resonance signal, thereby reducing resonance imaging contrast and spectral selectivity

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