Rice moderate leaf-rolling mutator RL14 and application thereof
A technology for mutating genes and rice, applied in the field of genetic engineering, can solve the problem of low utilization value and achieve the effect of promoting breeding research
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[0015] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.
[0016] Materials used in the preferred embodiment: wild-type Jinhui No. 10 and moderately rolled leaf mutant of rice rl14 Cultivated by Rice Research Institute of Southwest University; M-MLV reverse transcriptase, high-fidelity DNA polymerase PFU, T4 DNA ligase, Trizol kit, DNA gel recovery kit, plasmid extraction kit were purchased from TaKaRa Company; ampicillin ( Ampicillin, Amp) are products of Sigma; primer sy...
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