Rice moderate leaf-rolling mutator RL14 and application thereof

A technology for mutating genes and rice, applied in the field of genetic engineering, can solve the problem of low utilization value and achieve the effect of promoting breeding research

Inactive Publication Date: 2013-04-03
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the rice curling materials that have been found so far have great differences in the degree of curling of the leaves, and most of them are of low value in production.

Method used

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  • Rice moderate leaf-rolling mutator RL14 and application thereof
  • Rice moderate leaf-rolling mutator RL14 and application thereof
  • Rice moderate leaf-rolling mutator RL14 and application thereof

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Embodiment Construction

[0015] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0016] Materials used in the preferred embodiment: wild-type Jinhui No. 10 and moderately rolled leaf mutant of rice rl14 Cultivated by Rice Research Institute of Southwest University; M-MLV reverse transcriptase, high-fidelity DNA polymerase PFU, T4 DNA ligase, Trizol kit, DNA gel recovery kit, plasmid extraction kit were purchased from TaKaRa Company; ampicillin ( Ampicillin, Amp) are products of Sigma; primer sy...

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Abstract

The invention discloses a nucleotide sequence and a coding protein sequence of a rice moderate leaf-rolling mutator RL14, thus providing a powerful tool for rice transgenic research and promoting the breeding research on high-yield and high-quality rice.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, relates to a rice mutant gene, and also relates to the application of the mutant gene. Background technique [0002] Rice (Orvza sativa L.) is one of the most important food crops in the world, and nearly half of the world's population uses rice as a staple food. With the continuous increase of the world's population and the continuous reduction of arable land, food security has become a worldwide problem. Rice is a C3 plant with strong photorespiration and low effective photosynthetic rate, especially during the grain filling stage of rice in high temperature and drought areas. Due to the high temperature, its photorespiration will waste a lot of photosynthetic products, which will not only affect the yield, but also seriously affect Rice quality. Therefore, increasing the effective photosynthetic rate is an effective way to increase rice yield and improve rice quality. To achieve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/02A01H5/00
Inventor 何光华方立魁赵芳明桑贤春凌英华李云峰杨正林
Owner SOUTHWEST UNIV
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