Hybridization breeding method for hypsizygus marmoreus

A technology of mushrooms and strains, applied in the field of hybridization and breeding of mushrooms, can solve problems such as confusion in Chinese translation, difficulty in finding detailed information on biological characteristics of mushrooms, difficulty in popularizing and applying technologies and equipment, and achieve The effect of improving quality and yield and improving breeding efficiency

Inactive Publication Date: 2012-09-19
山东荣丰食用菌有限公司
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AI Technical Summary

Problems solved by technology

In the first few years of the introduction of Jadegrass strains to my country, due to the confusion of the Chinese translation of the name, foreign businessmen introduced the information of Shiji mushrooms very e

Method used

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with embodiment.

[0017] A method for hybridization and breeding of jitake mushrooms, comprising the following steps:

[0018] 1) First activate the mycelium, transfer the preserved strains RF24 and RF30 to 50 plates, and grow for 14 days;

[0019] 2) Select a high-quality culture plate for the two strains to optimize the culture, transfer mycelia to 50 plates respectively, and cultivate them under constant temperature and humidity conditions of 22 degrees for 14 days;

[0020] 3) Then take a high-quality optimized cultured plate for the two strains to purify the strains, transfer the mycelia to 50 plates, and cultivate them for 10 days. silk hybridization;

[0021] 4) After the hyphae are hybridized, culture them under constant temperature and humidity conditions at 22 degrees, and perform screening culture when the mycelia at the two inoculation points are combined and the plate is covered;

[00...

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Abstract

The invention discloses a hybridization breeding method for hypsizygus marmoreus. The method includes the steps of subjecting RF24 and RF30 to hypha activation, performing optimized cultivation on superior panels respectively, then subjecting the RF24 and the RF30 to strain purification on superior panels respectively, selecting exuberant at the top and purified two varieties for hypha hybridization after cultivation, performing screening cultivation when hyphae of two inoculation positions are combined and overgrow the panels after the hypha hybridization, selecting the panels which have the same hypha growing rate and combine together for expanding propagation cultivation after the screening cultivation, performing cultivation on the selected panels for 14 days after the expanding propagation, transferring to a conical flask for 37 days of cultivation and then transferring to a stock seed cultivation bottle for 45 days of growth, transferring to a cultivation bottle for 85 days and performing mycelium stimulation for fruiting, selecting the original strain with the highest yield for tissue isolation and mycelium cultivation, and preserving the strain to a test tube. The hybridization breeding method for the hypsizygus marmoreus can improve quality and yield of the hypsizygus marmoreus, greatly improve breeding efficiency, and is suitable for industrial cultivation of factory production.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for hybridization and breeding of mushrooms. Background technique [0002] True jiji mushroom, also known as jade mushroom, spot jade mushroom, lotus leaf lily mushroom, belongs to Basidiomycotina, layer fungi, agaricales, white mushroom family, and jade mushroom genus. It has the characteristics of beautiful appearance, unique flavor, and rich nutrition. It is a rare edible fungus that has become popular in the Japanese market in recent years and is favored by consumers. The shape of the mushroom is beautiful, the texture is crisp and tender, and the taste is delicious and unique. In Japan, people often compare it with the precious matsutake, and it is dubbed "false matsutake" and "crab-flavored mushroom", and enjoys the reputation of "matsutake when you hear it, and jade when you eat it". [0003] my country is in March, 1986, by the Dalian Branch o...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01H1/02
Inventor 封金华封金杰杨海军刘文钊
Owner 山东荣丰食用菌有限公司
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