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Immune magnetic particle for purifying aflatoxin samples and preparing method and application method thereof

A technology of aflatoxin and magnetic particles, which is applied in the purification process of aflatoxin samples, can solve problems such as low separation efficiency, complicated purification and separation operations, and potential safety hazards, and achieve high immobilization capacity, reduce damage and pollute the environment , the effect of avoiding contact

Active Publication Date: 2014-06-25
XIAN GOLDMAG NANOBIOTECH
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Problems solved by technology

[0009] The purpose of the present invention is to provide immunomagnetic microparticles for purifying aflatoxin samples and methods for their preparation and application, so as to solve the technical problems of complex purification and separation of aflatoxin samples in the background technology, low separation efficiency, and large potential safety hazards question

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  • Immune magnetic particle for purifying aflatoxin samples and preparing method and application method thereof

Examples

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Embodiment 1

[0041] Example 1 The preparation process of purifying aflatoxin immunomagnetic particles in the sample, and the specific process of purifying aflatoxin in the sample

[0042] 1) Preparation of immunomagnetic particles:

[0043] a. Immobilization of aflatoxin monoclonal antibody on the surface of isothiocyanate magnetic particles: Take 1 mg of the magnetic particle suspension and add it to a 2 ml centrifuge tube, shake gently to resuspend. Place on a magnetic separator, magnetically separate, and discard the supernatant. Add 1ml of coupling buffer (0.01M, PBS buffer at pH 7.4), shake gently to resuspend. Place on a magnetic separator, magnetically separate, and discard the supernatant. Repeat the operation once. Prepare 250 μg of aflatoxin monoclonal antibody into a solution with a certain concentration (0.01-10 mg / ml), add an appropriate amount to the magnetic particles, and react in a shaker at 37°C and 180 r / min for 45 minutes. After the reaction is completed, magnetic s...

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Abstract

The invention aims at providing an immune magnetic particle for purifying aflatoxin samples and a preparing method and an application method thereof so as to solve technical problems in the prior art that aflatoxin sample purification and separation is complex in operation and low in separation efficiency and has large potential safety hazard. After aflatoxin antibodies and magnetic particles are reacted together for some time, the immune magnetic particle with specificity is prepared. After coarsely extracted aflatoxins and the immune magnetic particle are reacted together for some time, the aflatoxins are attached to the surface of the immune magnetic particle in affinity mode. After magnetic separation, the aflatoxin samples can be purified so that the relative pure aflatoxin samples can be obtained, and further the relative pure aflatoxin samples are used for fluophotometers, high performance liquid chromatographs (HPLCs) and enzyme-linked immuno sorbent assay (ELISA).

Description

technical field [0001] The invention relates to a purification treatment process for aflatoxin samples, in particular to an immunomagnetic particle used for purifying aflatoxin samples and a preparation and application method thereof. Background technique [0002] Aflatoxin (AFT) is a secondary metabolite of fungi, mainly produced by Aspergillus flavus, Aspergillus parasitica and Aspergillus tererii, and widely exists in soil, animals and plants, and various nuts, especially peanuts and walnuts. There are four main types of aflatoxins: B1, B2, G1, and G2, among which B1 is considered to be the strongest carcinogen and mutagen among chemical carcinogens. In order to prevent food contaminated by AFT from entering the human consumption chain, timely detection will be an effective means. [0003] At present, the methods for determining aflatoxin (AFT) mainly include thin-layer chromatography, high performance liquid chromatography, enzyme-linked immunosorbent assay, mass spectr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N1/28
Inventor 崔亚丽王艳霞张秦鲁
Owner XIAN GOLDMAG NANOBIOTECH
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