Porcine parvovirus BQ-C strain and application of porcine parvovirus BQ-C strain to preparation of inactivated porcine parvovirus vaccine

A parvovirus and inactivated vaccine technology, applied in the field of biological vaccines, can solve problems such as reproductive obstacles and losses in pig farming

Active Publication Date: 2012-10-10
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since then, several strains of PPV have been isolated in various places. At present, it has been found that porcine parvovirus is often mixed with other viruses, such as porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus, resulting in multisystem syndrome in weaned piglets. syndrome, dermatitis, respiratory syndrome, etc., causing great losses to the pig industry in the world
Although different isolates belong to the same serotype, they can cause a variety of clinical symptoms, but they all mainly cause reproductive disorders

Method used

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  • Porcine parvovirus BQ-C strain and application of porcine parvovirus BQ-C strain to preparation of inactivated porcine parvovirus vaccine
  • Porcine parvovirus BQ-C strain and application of porcine parvovirus BQ-C strain to preparation of inactivated porcine parvovirus vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation and culture identification of porcine parvovirus BQ-C strain

[0023] 1 Source and standard of poisonous species

[0024] According to the declaration requirements of new biological products, combined with a large amount of test data obtained by the present invention, and with reference to "Chinese Veterinary Pharmacopoeia" (2005 edition), the poisonous species used for seedling production has been identified, and the poisoned species standard of the trial procedure (draft) is now carried out as follows illustrate.

[0025] 1.1 Source of poisonous seeds

[0026] The virus species used to manufacture this product is porcine parvovirus BQ-C strain, which the inventor obtained after isolation and domestication from diseased pigs in 2005. Since this virus strain is obtained from pregnant sows infected by typical porcine parvovirus It is isolated from the weak piglets produced, and compared with other isolated strains, it has a higher proliferation tite...

Embodiment 2

[0053] The preparation of embodiment 2 inactivated vaccines

[0054] The preparation process of porcine parvovirus inactivated vaccine (BQ-C strain) is prepared according to the current mature commercial vaccine preparation process. Judging from the quality inspection results of 3 batches of vaccines prepared in the laboratory and 5 batches of intermediate trial-produced vaccines, the quality of the vaccine products prepared by this process is stable, and the efficacy inspection results fully meet the established quality standards.

[0055] 1 Preparation of virus solution

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Abstract

The invention discloses a porcine parvovirus BQ-C strain and application of the porcine parvovirus BQ-C strain to the preparation of an inactivated porcine parvovirus vaccine, and belongs to the field of biological vaccines. The porcine parvovirus BQ-C strain has the advantages of high immunogenicity, good cultural character and the like; and porcine parvovirus (BQ-C strain) are inoculated to swine testis (ST) cells, a culture is harvested and inactivated by using binary ethylenimine (BEI), and the culture and a commercial MontanideTM ISA15AVG adjuvant of SEPPIC are mixed and emulsified to form the inactivated porcine parvovirus vaccine. The experiment proves that: diseases caused by porcine parvoviruses can be prevented by immunizing primiparous healthy female sows by using the prepared inactivated porcine parvovirus vaccine; and moreover, the invention has the advantages that the vaccine is safe, an antibody can be quickly generated, the immune period is long-lasting, and the like.

Description

technical field [0001] The invention relates to a porcine parvovirus and its application in preparing porcine parvovirus inactivated vaccine, in particular to a porcine parvovirus BQ-C strain isolated by itself and an inactivated vaccine prepared from the virus strain. It belongs to the field of biological vaccines. Background technique [0002] Porcine parvovirus (Porcine parvovirus, PPV) is one of the main pathogens that cause reproductive disorders in pigs. PPV infects mainly pregnant pigs, especially primiparous sows are susceptible to infection before pregnancy, causing abortion, stillbirth, Fetal mummification, etc., and the mother usually lacks clinical symptoms; of course, other symptoms can also be caused. Since Mayr and Mahnel discovered and confirmed the existence and pathogenicity of PPV in 1966, scholars at home and abroad have conducted extensive and in-depth research on it. In recent years, PPV infection has been expanding and rising, which has caused huge e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/23A61P31/20C12R1/93
Inventor 崔尚金
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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