Abelmoschus manihot extract and novel application of chemical components thereof

A technology of hollyhock flower and extract, which is applied in the field of hollyhock flower extract to treat or prevent diseases or diseases related to oxidative damage, and can solve problems such as damage and DNA nucleic acid strand breaks

Active Publication Date: 2012-10-17
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the half-life of reactive oxygen species is very short, due to its active chemical properties, it can react with DNA in a short period of time, causing DNA nucleic acid strand breaks and oxidative damage, forming DNA crosslinks

Method used

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  • Abelmoschus manihot extract and novel application of chemical components thereof
  • Abelmoschus manihot extract and novel application of chemical components thereof
  • Abelmoschus manihot extract and novel application of chemical components thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the preparation of extract of the present invention and formula I compound

[0053] Step 1: Take the dried flowers (2.8kg) of hollyhock flowers, heat and reflux with 10, 8, and 8 times the amount of 95% EtOH solution for extraction for 3, 2, and 2 hours successively, combine the filtrates, recover the solvent under reduced pressure, and vacuum at 50°C Dry to obtain 95% ethanol extract (648 g, 23.14%), which can be used as the reagent in the following test examples.

[0054] Step 2: Take 500g of the 95% ethanol extract obtained in the above step 1, and process it with D101 macroporous adsorption resin (water → 70% EtOH → 95% EtOH) to obtain water eluate (220g, 10.2%), 70% ethanol Eluate (120 g, 5.55%) and 95% ethanol eluate (22 g, 1.02%). Each eluate can be used as the reagent in the test examples below, especially the 70% ethanol eluate can be used as the reagent in the test examples below.

[0055] Step 3: The 70% ethanol eluate (96 g) obtained in the a...

Embodiment 2

[0057] Example 2: Structural identification and physicochemical properties of marshmallow anthoside C

[0058] Marshmallow anthoside C obtained in step 4 of Example 1: amorphous yellow powder. Under the 254nm ultraviolet light, there is a yellow dark spot, and the color of 10% sulfuric acid ethanol solution is brownish yellow, which may be flavonoids. Its specific rotation is negative ([α] D 25 : +49.0°, MeOH). High-resolution Q-TOF-ESI-MS gives its quasi-molecular ion peak m / z 509.0554[M-H] - , determine its molecular formula as C 21 h 18 o 15 . The information provided by the infrared spectrum shows that there is a hydroxyl group in the structure (3342cm-1 ), unsaturated ketone group (1652cm -1 ), aromatic ring (1607, 1511, 1418cm -1 ). Ultraviolet spectrum further suggested the existence of flavonol skeleton (305, 255nm). exist 1 H-NMR (400MHz, DMSO-d 6 ) in the low-field area of ​​the spectrum δ12.44 (1H, br.s) suggests that there is a phenolic hydroxyl group ...

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PUM

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Abstract

The invention relates to an abelmoschus manihot extract and a novel application of chemical components thereof, concretely, relates to a compound of formula I or its pharmaceutically acceptable salt, solvate, ester and prodrug, and an extract containing the compound of formula I, a composition thereof and an application thereof. The compound or extract of the invention can be used as anti-oxidative damaging agent, and can be used for treating or preventing diseases or symptoms related to oxidative damage.

Description

technical field [0001] The present invention relates to the field of traditional Chinese medicine chemistry, relates to a flavonoid compound obtained by extracting from hollyhock flower, and also relates to the new application of the flavonoid compound and the hollyhock flower extract containing the flavonoid compound, especially the use as an anti-oxidative damage agent and Use for treating or preventing a disease or condition associated with oxidative damage. Background technique [0002] Oxidative damage is an important factor leading to many chronic diseases, such as cardiovascular disease, cancer, liver disease, diabetes and aging. Oxygen produces active oxygen through one-electron reduction reaction in organisms, which participates in the physiological process of the organism and plays an important role in the metabolism and energy production of the organism. When reactive oxygen species are present, superoxide dismutase converts them into oxygen, effectively preventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07A61K31/7048A61P1/16A61P3/10A61P9/00A61P35/00A61P39/06
Inventor 王涛张祎刘二伟高秀梅胡利民李春梅韩立峰尚海花
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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