A method for testing the efficacy of duck hemorrhagic ovariitis inactivated vaccine

A technology for hemorrhagic oophoritis and inactivated vaccine, which is applied in the field of veterinary biological product inspection to achieve the effect of strong operability and objective method

Active Publication Date: 2015-12-09
INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are no detailed reports on objective, feasible, operable and scientific vaccine efficacy evaluation methods

Method used

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  • A method for testing the efficacy of duck hemorrhagic ovariitis inactivated vaccine

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Effect test

Embodiment 1

[0023] Example 1 Duck Hemorrhagic Ovariitis Inactivated Vaccine Efficacy Testing Age in Days of Ducks

[0024] The Peking ducks aged 42, 50, 100, 120, 200, 300, 400, 560 and 600 days were divided into 9 groups, 10 in each group, and each group was Peking ducks of the same age (purchased from Beijing Jinxing Duck Co., Ltd. Center Nankou breeding duck farm, duck hemorrhagic ovariitis antibody was negative). The test ducks were challenged with the same dose of DHOV-HB strain virus by oral route, containing about 3×10 4 ELD 50 Duck blood was collected through the wing vein 5 days after challenge, and the serum was separated aseptically. Serum was inoculated with 6-day-old SPF chicken embryos via the yolk sac route, 5 eggs / duck, 0.2ml / embryo, and incubated at 37°C for 168 hours. Chicken embryos that died within 24 hours were discarded, and the number of chicken embryos that died within 24 to 168 hours and were positive for DHOV by RT-PCR was calculated. One or more of the 5 chi...

Embodiment 2

[0028] Embodiment 2 Duck hemorrhagic ovariitis inactivated vaccine efficacy evaluation with the sex of the duck

[0029] Eighty 120-day-old Peking ducks of equal male and female were challenged orally with the same dose of DHOV-HB strain virus, containing approximately 3×10 4 ELD 50 / only. From 1 to 8 days after the challenge, the blood of ducks was collected through the wing vein, 10 ducks per day, and the serum was separated aseptically. Serum was inoculated with 6-day-old SPF chicken embryos via the yolk sac route, and 5 chicken embryos were inoculated with serum from each duck, 0.2ml / embryo, and incubated at 37°C for 168 hours. Chicken embryos that died within 24 hours were discarded, and the number of chicken embryos that died within 24 to 168 hours and were positive for DHOV was calculated. One or more of the 5 chicken embryos in each serum was dead and DHOV positive was recorded as the duck virus isolation positive. The positive rate of virus isolation in female Pek...

Embodiment 3

[0030] Example 3 Immune protection of duck hemorrhagic ovariitis inactivated vaccine efficacy evaluation-virus isolation method (1)

[0031] Use the inactivated duck hemorrhagic ovariitis vaccine (batch number 2012001, developed by the Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences and Ruipu (Baoding) Biopharmaceutical Co., Ltd.) to immunize 10 140-day-old Peking ducks, all female, as a vaccine immunization Group. The immunization dose and method of the vaccine immunization group are as follows: the vaccine dose for the first immunization is 1ml / bird, and the chest muscle is injected. Two weeks after the first immunization, follow the same dose and the same way for the second immunization, and challenge the virus 4 weeks after the second immunization. The challenged virus strain is DHOV-HB strain, and the challenged virus is administered orally. The challenged dose for each duck is about 3×104 ELD 50 . At the same ...

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Abstract

The invention provides a test method for efficacy of duck hemorrhagic ovaritis inactivated vaccine. The method comprises steps of: immunizing ducks using the duck hemorrhagic ovaritis inactivated vaccine for two times, wherein the second immunization is carried out two weeks after the first immunization; challenging the immunized ducks with duck hemorrhagic ovaritis virus three to five weeks after the second immunization; collecting duck serum after the challenge; inoculating the isolated serum into SPF chicken eggs or duck eggs; and calculating the number of eggs which die within 24-168 hours and are tested positive for the duck hemorrhagic ovaritis virus, wherein if the positive incidence of the isolated virus is more than 80% in contrast group ducks while the negative incidence of the isolated virus is more than 70% in the immunized group ducks, the vaccine efficacy is determined to pass the test. The test method for efficacy of duck hemorrhagic ovaritis inactivated vaccine of the invention has the advantages of simple in operation and accurate in test results, and thereby can be wildly used.

Description

technical field [0001] The invention relates to a method for testing the efficacy of inactivated vaccines for veterinary use, in particular to a method for testing the efficacy of inactivated vaccines for duck hemorrhagic oophoritis, which belongs to the technical field of testing biological products for animals. Background technique [0002] Duck Hemorrhagic Ovaritis (DH) is a newly popular, acute and highly contagious duck infectious disease. Its pathogen is Duck Hemorrhagic Ovaritis Virus (DHOV), which belongs to flavivirus Members of the family infected ducks of different ages, causing ovarian hemorrhage, testicular atrophy, egg production, fertilization rate and hatching rate of sexually mature ducks, and increased duck death rate. [0003] In the study of the taxonomic status of the virus, through the sequence homology analysis and evolution analysis of the genome sequence of the F4 generation duck embryo passage virus of the isolated virus strain and known viruses, it...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/15
Inventor 刘月焕杨保收韩春华何平有林健郁宏伟朱秀同刘浩毛雅园徐倩倩赵际成段会娟潘洁梁武
Owner INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI
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