Rabbit haemorrhagic disease tissue inactivated vaccine and preparation method thereof
An inactivated vaccine and tissue technology, which can be used in pharmaceutical formulations, inactivation/attenuation, medical preparations containing active ingredients, etc. It can solve the problems of economic losses in rabbit farms, rabbit plague in rabbit populations, etc., and enhance immunity. Antibody production and enhancement of leukocyte phagocytic ability
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Embodiment 2
[0023] The rabbit plague tissue inactivated vaccine of this embodiment contains rabbit plague tissue virus liquid and propolis adjuvant, and the addition ratio is: 10 mg of propolis is contained in each milliliter of virus extract.
Embodiment 3
[0025] The rabbit plague tissue inactivated vaccine of this embodiment contains rabbit plague tissue virus liquid and propolis adjuvant, and the addition ratio is: 5 mg of propolis is contained in each milliliter of virus extract.
[0026] Preparation method embodiment of rabbit plague tissue inactivated vaccine
[0027] The preparation method of the rabbit plague tissue inactivated vaccine of the present embodiment is as follows:
[0028] 1) Treatment of diseased materials: autopsy dead rabbits with typical symptoms of rabbit plague, aseptically take more than half of the liver and add sterile saline at a ratio of 1:5, plus penicillin and streptomycin 1000U (ug) / ml, and place for 4 Centrifuge at 4000rpm / min for 5 minutes, freeze and thaw 3 times at 10000rpm / min for 5 minutes, take the supernatant and purify it by centrifugation at 10000rpm / min for 30 minutes at 4℃, and store the supernatant aseptically for later use.
[0029] 2) Rabbit subculture: 1ml / mouse of the above-ment...
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