Chinese medicine compound or composition of its extract and application thereof
A composition and extract technology, which can be applied in the direction of drug combination, pharmaceutical formula, medical preparation containing active ingredients, etc., can solve the problem of lack of pharmaceutical preparations and the like
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[0028] Preparation technology of traditional Chinese medicine compound and its preparation
[0029] Preparation of traditional Chinese medicine compound
[0030] After the ginseng, coptis and gardenia decoction pieces with a weight ratio of 3:2:2-0.5 are crushed, the Coptis decoction pieces powder is reflux extracted with 50% ethanol for 3 hours each time, and the ethanol extract is concentrated and the pH is adjusted with concentrated hydrochloric acid. 2. Add 10%-12% NaCl solid, stir well to dissolve it completely, let stand overnight, filter with suction, wash the precipitate with a small amount of distilled water, and dry at 50-60°C to obtain Coptidis Rhizome extract. Then decoct the ginseng and gardenia decoction pieces powder with water four times, 2 hours each time, adding 12 times the amount of medicinal materials; combine the decoctions, filter, and concentrate to a clear paste with a relative density of 1.04-1.05 (50°C) , add ethanol to make the alcohol content reac...
Embodiment 1
[0055] Example 1: The composition of the present invention has a protective effect on animals with acute focal cerebral ischemia.
[0056] Animal Model Establishment of Acute Focal Cerebral Ischemia
[0057] Male SD rats with a body weight of 250-270 g were used, referring to the large scale established by Zea Longa et al. (Longa EZ, Weinstein PR, Carlson S, Cummins R. The method of occluding the middle cerebral artery of rats with a suture was injected intraperitoneally with 400 mg / kg of 10% chloral hydrate solution to anesthetize the animal. Rats were fixed in the supine position, the skin was incised in the middle of the neck, and all layers of tissue were bluntly separated to expose the right common carotid artery (CCA). After the bifurcation of the internal carotid artery (ICA) and external carotid artery (ECA), it was separated carefully to avoid damage to the vagus nerve and trachea, and the line was placed for use. The ipsilateral external carotid artery was separate...
Embodiment 2
[0071] Embodiment 2 Composition of the present invention is to senile dementia (AD) animal protective effect research experiment
[0072] β-amyloid (Aβ 1-40 ) incubation
[0073] Aβ with sterile saline 1-40 Dilute to 2ug / ul, incubate at 37°C for 1 week to make it into condensed Aβ 1-40 .
[0074] Aβ 1-40 Establishment of rat dementia model
[0075] Take 250-270g healthy male AD rats and fix them on the rat stereotaxic apparatus after anesthesia, routine skin preparation and disinfection, incise the skin, expose the bregma, and use a micro-injection syringe to the left hippocampus of the rats (with Bregma point as 0 point) , AP-3.0mm, ML2.0mm, DV-2.9mm) slowly inject condensed Aβ 1-40 5 μl, retain the needle for 5 minutes to fully diffuse Aβ, then withdraw the needle slowly, and suture the incision. In the sham operation group, only preoperative anesthesia was performed, the skin was incised to expose the bregma, and Aβ was not injected 1-40 .
[0076] Experimental gro...
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