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PPAR (Peroxisome Proliferator Activated Receptor) alpha gene and method of applying PPAR alpha gene as goose fatty deposition related genetic marker

An alpha gene and gene technology, which is applied in the molecular biology technology and breeding field of goose, can solve the problems of no research report on goose PPARalpha gene and few chickens, and achieve the effect of great application value and economic benefit.

Inactive Publication Date: 2015-04-29
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, compared with chickens, there are few studies on the DNA genetic markers, genetic diversity and related functional genes of goose, and there is no research report on goose PPARα gene.

Method used

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  • PPAR (Peroxisome Proliferator Activated Receptor) alpha gene and method of applying PPAR alpha gene as goose fatty deposition related genetic marker
  • PPAR (Peroxisome Proliferator Activated Receptor) alpha gene and method of applying PPAR alpha gene as goose fatty deposition related genetic marker
  • PPAR (Peroxisome Proliferator Activated Receptor) alpha gene and method of applying PPAR alpha gene as goose fatty deposition related genetic marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Goose related gene SNP screening

[0023] 200 commercial geese of the matching line of healthy chick Xupu geese at the same age were selected for feeding experiment. The high-abdominal fat type and low-abdominal fat type of these geese were identified, and the genomic DNA was extracted from the samples of the high-abdominal fat type and the low-abdominal fat type.

[0024] According to the mRNA sequence of the chicken PPARα gene in GenBank, Primer 5.0 was used to design primers to cover the partial coding sequence of the gene. , synthesized by Shanghai Yingwei Chuangjin Company. The above primers were used to amplify the extracted goose genomic DNA respectively.

[0025] PCR: 10μl Reaction system: 10×buffer (containing Mg 2+ ) 1 μl, upper and lower primers (20 μmol / L) each 0.15 μl, dNTPs (10 μmol / L) 0.2 μl, Taq enzyme 1U, DNA (100ng) 0.7 μl, ultrapure water to the required volume.

[0026] PCR amplification program: 30 PCR cycle parameters after pre-denatu...

Embodiment 2

[0033] Example 2 Verification of SNPs related to abdominal fat rate in production practice

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Abstract

The invention discloses a PPAR (Peroxisome Proliferator Activated Receptor) alpha gene and an application of the PPAR alpha gene serving as a goose fatty trait genetic marker. Genetic markers in the PPAR alpha gene, which are related to the abdominal fat weight and abdominal fat percentage of a goose are determined, the existence of polymorphism of the PPAR alpha gene in a nucleic acid sample obtained from the goose is determined, and the polymorphism results in the change of an encoded nucleic acid and is related to the abdominal fat weight and the abdominal fat percentage. The single nucleotide polymorphism of the gene can be used for carrying out fatty trait breeding on animals, genome DNAs (deoxyribonucleic acids) of which contain the PPAR alpha gene, so that a dominant genotype capable of reducing the abdominal fat weight and abdominal fat percentage of the goose is screened out; and the PPAR alpha gene has very great application value and economic benefits.

Description

technical field [0001] The present invention relates to the field of molecular biology technology and breeding of goose, specifically relate to the existence of the hereditary marker of the feature related to determination and abdominal fat weight, abdominal fat rate, more specifically, it is to measure the polymorphism in the PPARα gene, namely with The existence of single nucleotide polymorphism (Single nucleotide polymorphism, abbreviated as SNP) of goose peroxisome proliferator-activated receptor (PPAR) gene related to abdominal fat weight and abdominal fat rate. Background technique [0002] The development of modern poultry genetic breeding theory and application has greatly improved the growth rate and feed efficiency of broiler poultry, especially broiler chickens and ducks, and the production efficiency has gradually adapted to industrial production. But it is also easy to bring a large amount of deposition of carcass abdominal fat and subcutaneous fat, resulting in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C12Q1/68
Inventor 曲湘勇何俊蒋隽贺长青
Owner HUNAN AGRICULTURAL UNIV
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