Kit for detecting or assisting in detecting ethambutol resistance of multiple drugs resistant bacillus tuberculosis
A technology of Mycobacterium tuberculosis and ethambutol, which is applied in the detection/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of high treatment costs, high mortality, and long treatment cycles, and achieve Effective early rapid diagnosis and high diagnostic sensitivity
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Embodiment 1
[0031] Example 1. Obtaining of the ethambutol resistance kit for detection or auxiliary detection of multidrug-resistant Mycobacterium tuberculosis
[0032] 1. Acquisition of multidrug-resistant Mycobacterium tuberculosis ethambutol resistance-related gene mutation sites
[0033] (1) Genomic DNA was extracted, as follows:
[0034] The sputum samples of 61 tuberculosis patients from the Beijing Institute of Tuberculosis Control and 110 tuberculosis patients from the Shanghai Center for Disease Control and Prevention who were identified as resistant to rifampicin (RIF) and isoniazid (INH) Smear, culture, isolation, purification, etc. These tuberculosis patients were all infected with multidrug-resistant Mycobacterium tuberculosis (MDR Mycobacterium tuberculosis), and MDR-resistant Mycobacterium tuberculosis (slender and slightly curved bacilli, 1 to 4X0.4μm), save for future use;
[0035] Resuscitate the MDR Mycobacterium tuberculosis isolated and purified above in a biosafety...
Embodiment 2
[0063] Example 2, Application of the Ethambutol Resistance Kit for Detection or Auxiliary Detection of Multidrug-resistant Mycobacterium tuberculosis
[0064] The samples were: 61 tuberculosis patients identified as ethambutol-resistant and 21 tuberculosis patients identified as non-ethambutol-resistant from Beijing Institute of Tuberculosis Control, Shanghai Center for Disease Control and Prevention Sputum samples from 110 TB patients identified as ethambutol-resistant and 45 TB patients identified as non-ethambutol-resistant; these TB patients were all infected with multidrug-resistant Mycobacterium tuberculosis, That is, all patients were resistant to rifampicin (RIF) and isoniazid (INH) after testing, and all patients who underwent the following experiments were informed.
[0065] Method 1: Detection of ethambutol resistance at 4 mutation sites
[0066] 1. Separate and purify the sputum samples of each patient in the above-mentioned regions to obtain multidrug-resistant M...
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