Primer and kit for identifying foot-and-mouth disease and immunity of entry animal

Abstract

The invention provides a kit for identifying foot-and-mouth disease and immunity of an entry animal. The kit comprises three pre-coated ELISA (Enzyme Linked Immunosorbent Assay) microwell plates, a goat anti-swine antibody-horseradish peroxidase conjugate, a washing solution, a substrate, a color developing agent, a sample diluent, a stop solution, a positive reference substance and a negative reference substance, wherein the three pre-coated ELISA microwell plates are respectively pre-coated by a foot-and-mouth disease 3ABC antigen under multi-copy showing on the surface attached by T4 phage, a foot-and-mouth disease 3B antigen under multi-copy showing on the surface attached by T4 phage, and a foot-and-mouth disease 3D antigen under multi-copy showing on the surface attached by T4 phage; the washing solution is 0.15mol / L of PBS (Phosphate Buffer Solution) with pH (Potential Of Hydrogen) of 7.2; the substrate is 0.1% of TMB (Tetramethyl Benzidine) solution; the color developing agent is 0.015% of H2O2; the sample diluent comprises 0.1% of bovine serum albumin and 0.1% of sodium azide which serves as a protective agent; the stop solution is 2mol / L of H2SO4; the positive reference substance is diluted by 500 times and adopts 0.1% of sodium azide as the protective agent; and the negative reference substance is diluted by 500 times and adopts 0.1% of sodium azide as the protective agent.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a domestic animal foot-and-mouth disease infection and immune identification kit. Background technique [0002] Foot-and-mouth disease (FMD), also known as aphthous fever (Aphthous fever), is an acute, febrile, high-level contact disease caused by cloven-hoofed animals infected by foot-and-mouth disease virus (FMDV). STDs. In addition to major domestic animals such as pigs, cattle, and sheep, FMDV can also infect more than 30 kinds of wild animals, and the incidence rate after infection is almost as high as 100%. Blisters appear on the mouth, tongue, hooves and breasts of sick animals, ulcerate and form rotten spots. People can also be infected with FMD, the symptoms are mild, and most of the course of the disease is benign. All countries in the world attach great importance to the FMD epidemic, and the World Organization for Animal Health (OIE) and the World Food and Agriculture ...

AI Technical Summary

Problems solved by technology

[0005] However, the existing detection methods still have shortcomings and deficiencies, mainly as follows: ① The multi-copy NSP antigen displayed on the surface of phage is not used; ② The antigen separation and purification process is complicated

Images