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Manufacturing method of scallop color fan for measuring total carotenoid content in adductor muscles of scallops and application of scallop color fan

A carotene and production method technology, applied in the field of colorimetric tools, can solve the problems of cumbersome methods, high cost, poor practicability and the like, and achieve the effects of strong operability, strong environmental protection and strong practicability

Active Publication Date: 2013-05-01
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has many disadvantages: 1) The process is complex and time-consuming, and it usually takes one week from sampling, drying, grinding, extraction, centrifugation, analysis to the completion of determination; 2) There are many and expensive instruments and equipment, including expensive vacuum freezing Dryer, UV spectrophotometer, high-speed centrifuge, grinder, pipette and other equipment; 3) Organic solvents are needed for chemical extraction of total carotenoids, which is not only costly, but also easy to pollute the environment; 4) Poor practicability: no On-site measurement can be carried out, but it must be carried out in the laboratory; the method is cumbersome, requires professionals to operate, and cannot be applied in practice
However, so far, there has not been any report on the quick and easy determination of total carotenoid content in shellfish

Method used

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  • Manufacturing method of scallop color fan for measuring total carotenoid content in adductor muscles of scallops and application of scallop color fan

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Experimental program
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Effect test

Embodiment 1

[0050] like figure 1 As shown, the scallop color comparison fan made by the present invention includes a fan body 1 and a fan handle 2, and the fan body 1 includes a color area 3 and a numerical value area 4, and the color area 3 includes 10 spaced from left to right Color standard gradient; the numerical value area 4 includes 10 numerical values, and the numerical values ​​are 10, 30, 50, 70, 100, 130, 160, 190, 220 and 250 from left to right, and the unit is μg / g, respectively 10 standard color gradients corresponding to the color area 3; the fan body 1 is connected to the fan handle 2.

[0051] The 10 color standard gradients of the color area 3 are all single golden yellow, and the color changes from light to dark from left to right.

[0052] The 10 numerical values ​​in the numerical value area 4 represent the total carotenoid content, and the unit is μg / g.

[0053] The 10 numerical values ​​representing the total carotenoid content in the numerical value area 4 are adj...

Embodiment 2

[0059] The preparation method of scallop color comparison fan of the present invention is as follows:

[0060] Time: May 2012

[0061] 1) Take 20 live scallops, the shells are all golden yellow, and the color of adductor muscle varies from light yellow to dark yellow;

[0062] 2) Take pictures of the scallop adductor muscle under the same conditions, and use Photoshop to obtain the R, G, and B values ​​of the three primary colors of each individual;

[0063] 3) Determination of the total carotenoid content TCC (Total Carotenoids Content) in the adductor muscle of each individual by conventional methods (Zheng et al., 2010);

[0064] 4) Use Matlab software to linearly fit the above R, G, B values ​​and TCC values ​​respectively, and increase the TCC standard value with a gradient of 10 to obtain standard R, G, B values;

[0065] 5) Input the above values ​​into Photoshop to obtain the color block corresponding to the TCC standard value;

[0066] 6) Print 10 color blocks from...

Embodiment 3

[0091] Time: July 2012.

[0092] 1) One 1.5-year-old living Chlamys scallop (number: #3), open its two shells;

[0093] 2) Use the scallop colorimetric fan to compare the color of the scallop adductor muscle;

[0094] 3) It is obtained that the total carotenoid content of #3 individual is 100~130μg / g, which takes 4 seconds.

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Abstract

The invention relates to a manufacturing method of a scallop color fan for measuring the total carotenoid content in adductor muscles of scallops and application of the scallop color fan. The content range of the total carotenoid in the adductor muscles of each scallop can be obtained within 3-5 seconds by comparing the manufactured scallop color fan with the adductor muscle color of the scallop. The content range of the measured total carotenoid in the adductor muscles of Chlamys nobilis Reeve is 10-250 Mu g / g. The manufacturing method of the scallop color fan for measuring the total carotenoid content in adductor muscles of scallops is simple and strong in operability. A method for measuring the measured total carotenoid in the adductor muscles of Chlamys nobilis Reeve is rapid, simple, convenient, high in efficiency, low in cost, easy to popularize, strong in practicability and strong in environmentally-friendly property.

Description

technical field [0001] The present invention relates to a method for making a colorimetric tool for measuring the total carotenoid content of marine shellfish and its application, in particular to a method for making a scallop colorimetric fan for measuring the total carotenoid content in the adductor muscle of a scallop and its application. its application. Background technique [0002] Carotenoids (carotenoids), also known as lipid pigments, belong to terpenoids, usually 40 carbon hydrocarbons (carotene) and their oxidized derivatives (lutein). Synthesized by algae and microorganisms, it exists in nature in the form of yellow, orange-red or red pigments, and 750 species have been discovered so far. [0003] Since carotenoids are widely distributed and have various functions and special properties, since the yellow β-carotene was first isolated from carrots in 1831, carotenoid research has always been one of the most interesting fields. Carotenoids have bright color and h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 郑怀平李荣宗刘合露林钊鹏杨林锋张涛
Owner SHANTOU UNIV
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