Establishment of PCR-HRM analysis method for rapid differential diagnosis of different serotypes of avian leukemia viruses
A kind of avian leukosis virus and virus technology, applied in the field of virus research, can solve the problems of inability to achieve high-throughput detection tasks, expensive subgroup-specific probes, and limitations on the practical application of fluorescent quantitative PCR detection methods, and achieve good expansion Increased, reduced time, good specific effect
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[0060] Below in conjunction with embodiment, further illustrate the present invention.
[0061] Extraction of Avian Leukosis Virus RNA:
[0062] The kit MiniBEST Viral RNA / DNA Extraction Kit Ver.4.0 was used to extract the avian leukosis virus RNA in the disease samples. Disease samples can be whole blood, plasma, egg white, cloacal swabs and other samples that are easy to obtain and have no serious harm to the animal body. Disease samples can also be tissue samples, such as spleen and thymus.
[0063] Reverse transcription of avian leukosis virus RNA:
[0064] Use the M-MLV RTase cDNA Synthesis Kit reverse transcription kit to reverse transcribe the avian leukosis virus RNA (in order to further ensure the subsequent HRM analysis and simplify the comparative analysis of the results, the concentration of the cDNA obtained by the reverse transcription can be measured with a nucleic acid protein analyzer. Make the PCR starting template concentration relatively consistent). ...
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