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Enzymolysis method for preparing velvet antler collagen

A collagen and deer antler technology is applied in the field of enzymatic hydrolysis for preparing deer antler collagen, which can solve problems such as waste of deer antler resources and achieve the effect of convenient application.

Inactive Publication Date: 2013-06-26
栾清杨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the traditional way of utilizing velvet antler is a great waste of velvet antler resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take a certain amount of 1 kilogram of fresh velvet antler, add 3000 milliliters of lactate buffer, grind it with a tissue grinder, add biocatalyst: 1 gram of papain, add activator: 0.1 gram of disodium hydrogen phosphate, adjust the pH value to 8.0, adjust the temperature of the solution to 52°C, put it in a reaction tank for 3 hours, centrifuge to remove the residue, and collect 3080 ml of transparent and clear liquid.

Embodiment 2

[0019] Take a certain amount of fresh antler 1 kg, add 3000 ml of citrate buffer, grind it with a tissue grinder, add biocatalyst: 8 g of papain, add activator: 0.1 g of calcium chloride, adjust the pH value 7.0, adjust the temperature of the solution to 37°C, put it into the reaction tank for 5 hours, centrifuge to remove the residue, and collect 3300 ml of transparent and clear liquid.

Embodiment 3

[0021] Take a certain amount of 1 kilogram of fresh velvet antler, add 3000 milliliters of lactate buffer, grind it with a tissue grinder, add biocatalyst: 1 gram of trypsin, add activator: 0.1 gram of manganese chloride, and adjust the pH value 8.0, adjust the temperature of the solution to 52°C, put it into the reaction tank for 3 hours, centrifuge to remove the residue, and collect 3340 ml of transparent and clear liquid.

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PUM

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Abstract

The invention relates to an enzymolysis method for preparing velvet antler collagen. The enzymolysis method comprises the steps of: mincing fresh velvet antler in a buffer solution by using a tissue mincer, adjusting the pH value of the mixture to be 6.0-8.0, adjusting the temperature of the solution to be 20-35 DEG C, adding a compound biological catalyst for hydrolysis, and then carrying out centrifugal or diatomaceous earth filtration to obtain a clear golden velvet antler hydrolysate so as to obtain a product. The obtained product can be applied to various aspects such as pharmacy, health care products and foods, and is extremely convenient to apply. Simultaneously a plurality of new development approaches can be derived by utilizing the technology for developing velvet antler products, so that a series of new products can be obtained.

Description

technical field [0001] The invention relates to an enzymatic hydrolysis method for preparing deer antler collagen, which belongs to the technical field of extracting active ingredients of traditional Chinese medicine. Background technique [0002] Antler is the unossified and densely hairy young horns of the male deer (Cervus nippon Temminck) or red deer (Cervus elaphus Linnaeus) of the deer family. It was first recorded in "Shen Nong's Materia Medica". "Compendium of Materia Medica" states that velvet antler can "generate essence and replenish marrow, nourish blood and benefit yang, strengthen muscles and bones, and cure all deafness, deafness, dark eyes, dizziness and dysentery". The chemical composition in deer antler is relatively complex, including amino acids, phospholipids, fatty acids, protein polypeptides, hormone-like substances, nucleic acids, and various inorganic elements. The analysis of chemical components in various strains of velvet antler shows that the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61P1/14A23L1/29A61K35/32A61K38/39A23L33/00
Inventor 魏强王宇菲
Owner 栾清杨
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