Anti-gosling plague egg yolk antibody and preparation method thereof

A technology of egg yolk antibody and goose plague defatted egg yolk, which is applied in the direction of antiviral immunoglobulin and immunoglobulin from eggs, etc., can solve the problems of inconvenient storage, complicated preparation process, and multiple protective agent components

Active Publication Date: 2013-06-26
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional egg yolk antibody preparation method has the following disadvantages: 1. The egg yolk contains some pathogens that can be transmitted vertically. If strict disinfection and inactivation are not carried out in the egg yolk antibody production, it can lead to artificial transmission of the virus
2. Bacteria commonly found on the surface of eggshells cannot be inactivated by crude methods; 3. High-concentration egg yolk liquid provides the most favorable conditions for the reproduction of contaminated bacteria; 4. Inconvenient storage and high storage costs; 5. Contains A large amount of macromolecular structure substances such as protein and phospholipids are likely to cause difficulty in absorption after injection, and will cause swelling, bleeding, etc., which will affect the effect of antibodies; 6. It is extremely inconvenient to use, because of its high viscosity, it is easy to block the syringe
Antibodies prepared by this method need to be stored at 2-8°C, and there are many protective agents in the freeze-drying process, the preparation process is complicated, and the cost is high

Method used

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  • Anti-gosling plague egg yolk antibody and preparation method thereof
  • Anti-gosling plague egg yolk antibody and preparation method thereof
  • Anti-gosling plague egg yolk antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Preparation of anti-gosling plague egg yolk antibody

[0060] 1. Preparation of non-polluted gosling venom

[0061] Dilute the virus seed with sterilized phosphate buffered saline (PBS) at 1:100, inoculate 12-day-old non-immune goose embryos through the allantoic cavity, 0.2ml per embryo, and incubate at 38-38.5°C after inoculation without turning the eggs. Eggs were illuminated once a day after inoculation, and dead embryos within 48 hours were discarded, and eggs were illuminated every 8 hours thereafter. Dead embryos were harvested at 72h-120h and cooled at 4°C for 24h. The cooled goose embryos were placed in a biological safety cabinet, and the non-polluted goose embryo allantoic fluid was harvested, and samples were taken for sterility testing and virus content determination. Mix the aseptic goose embryo allantoic fluid and carry out dialysis and concentration to obtain non-polluted gosling venom; absorb and put the prepared non-polluted gosling venom i...

Embodiment 2

[0093] Example 2: Therapeutic effect test of refined egg yolk antibody against gosling plague

[0094] Three batches of 01, 02, and 03 gosling plague egg yolk antibodies were injected into 40 1-day-old, 10-day-old, and 20-day-old goslings respectively, and each gosling was injected with 0.5ml. 48h, 96h, 144h, 192h after injection, respectively use 100LD 50 10 virulent injections each, 10 challenge controls and 10 blank controls were set up for each challenge, kept in isolation, observed for 10 days, and recorded the death of goslings. The results are shown in Table 1, Table 2, and Table 3.

[0095] Table 1 Experimental results of 1-day-old goslings

[0096]

[0097] Table 2 Experimental results of 10-day-old goslings

[0098]

[0099] Table 3 Experimental results of 20-day-old goslings

[0100]

[0101] The test results showed that 3 batches of refined egg yolk antibodies against gosling plague were injected into goslings of different ages, and the relative protect...

Embodiment 3

[0102] Embodiment 3: the shelf life test of egg yolk antibody

[0103] The storage effect of the product under different conditions was measured. Under the condition of reducing the storage cost and ensuring that the antibody titer does not decrease, the product can be stored at 20-25°C, and the storage period is 24 months. The specific shelf life test results are shown in Table 4.

[0104] Table 4 The test results of the storage period of gosling plague refined egg yolk antibody under different conditions

[0105]

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Abstract

The invention relates to an anti-gosling plague egg yolk antibody and a preparation method thereof, which adopt multiple inactivation technologies of physics, chemistry and the like and use the acidified water dilution method in combination with such modern biotechnics as the caprylic acid method, high speed centrifugal and superfiltration to effectively separate and purify immunoglobulin in egg yolk. The antibody contains no harmful substances and outer contaminations, has high safety and no residues at the injection position, has no irritation and toxic and side effects, and has no influence on carcass quality; the antibody is conveniently carried and needs no thawing when used, and is dissolved by using sterile saline solution to the volume prior to lyophilizing, and the injection of the antibody is convenient and fast; the antibody has high stress resistance and stable titer, and can be stored for long time; and the antibody is efficacious when stored for 24 months under the condition of room temperature, and the storage period of the antibody can be greatly prolonged under the condition of 2-8 DEG C. The antibody is a combined antibody, and has good prevention and treatment effect for multiple gosling infections such as gosling plague.

Description

technical field [0001] The invention relates to the technical field of poultry biopharmaceuticals, in particular to an anti-gosling plague egg yolk antibody and a preparation method thereof. Background technique [0002] my country is a big country producing waterfowl, especially the goose industry. In recent years, with the adjustment of the industrial structure, the goose industry has developed faster, but the disease is also increasing. Among a variety of goose infectious diseases, goose plague, goose avian influenza, goose paramyxovirus disease, goose hemorrhagic necrotizing hepatitis, and goose and duck plague virus infection all have high morbidity and mortality, often causing huge Economic loss seriously affects the healthy development of goose industry. Due to the occurrence of a variety of infectious diseases, the pattern of geese with few diseases and easy to raise geese has changed to the situation of more geese with fewer biological agents, high fatality rate a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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