Amplification composition and rapid detection kit used for trisomy 21 syndrome detection

Abstract

The invention relates to a PCR (polymerase chain reaction) amplification composition and rapid detection kit used for trisomy 21 syndrome detection. Ten short tandem repeat sequences (D21S1437, D21S2052, D21S11, D21S2055, D21S1411, D21S1432, D21S1435, LFG21, Penta D and D21S1413) on a chromosome 21 and two non-short tandem repeat sequences (Penta E and FGA) on the chromosome 21 are amplified at the same time in one PCR system. The PCR amplification composition and rapid detection kit system can be used for diagnosing numerical abnormalities of a chromosome 21 of human, the obtained product is provided with fluorescent marks as each locus adopts a primer marked by fluorescence, detection can be carried out on instruments such as a genetic analysis meter, the obtained fragment length is more accurate, and repeatability is higher. The PCR amplification composition and rapid detection kit used for trisomy 21 syndrome detection have the advantages that operation is easy, large-scale popularization is convenient, the whole process is automatic, and the whole detection process can be completed by about six hours only.

Description

Technical field [0001] The present invention relates to the field of biotechnology detection, in particular to a PCR amplification composition for trisomy 21 detection, involving 10 pairs of specific primers and a rapid detection kit thereof. Background technique [0002] Down's syndrome, also known as Trisomy 21 syndrome, is a disease caused by an increase in the number of chromosome 21. It is the most common chromosomal disease in humans. It was first reported by British doctor Langdon Down in 1866. Have done a clinical description, so it is called Down syndrome. The incidence of live births is about 1 / 600-1 / 800, and the total prevalence of trisomy 21 in my country is 0.47‰, of which 0.26‰ in urban areas and 0.56‰ in rural areas (Zhang Zhixiang et al., Trisomy 21 in China Epidemiological study of symptom, "Chinese Journal of Clinical Psychology", 1996 03). Children with trisomy 21 often have symptoms such as growth retardation, physical and intellectual impairment, and inabili...

AI Technical Summary

Problems solved by technology

Children with trisomy 21 often have symptoms such as growth retardation, physical and intellectual disabilities, and inability to take care of themselves. Their living conditions are poor and they cause a great burden to the family.

The above-mentioned patents often use unreasonable or small number of STR sites, and the number of sites contained in each QF-PCR amplification system is small, resulting in insufficient detection efficiency and accuracy

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