Technology for directly cultivating seedless momordica grosvenori by utilizing tetraploid momordica grosvenori female plants

A seedless Luo Han and Luo Han Guo technology, applied in the application, plant genetic improvement, angiosperm/flowering plants and other directions, can solve the problems of inability to apply, the fruit of Luo Han Guo becomes smaller and shorter, and achieves shortening of the breeding cycle and the utilization rate of the whole fruit. Increase, overcome severely diminished effects

Active Publication Date: 2013-07-24
怀化兴科创生物技术有限公司
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  • Application Information

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Problems solved by technology

[0005] The current seedless Luo Han Guo technology is to cross the diploid Luo Han Guo male plant with the triploid Luo Han Guo female plant to obtain seedless Luo Han Guo. The fruit of Luo Han Guo obtained by

Method used

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  • Technology for directly cultivating seedless momordica grosvenori by utilizing tetraploid momordica grosvenori female plants
  • Technology for directly cultivating seedless momordica grosvenori by utilizing tetraploid momordica grosvenori female plants
  • Technology for directly cultivating seedless momordica grosvenori by utilizing tetraploid momordica grosvenori female plants

Examples

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Effect test

Embodiment 1 4

[0034] Example 1. Cultivation of high-sweetness seedless Luo Han Guo derived from tetraploid female parent

[0035] 1. Take the stem tip of the female Luo Han Guo plant with a good phenotype in the field, wash it with running water for 2 hours, disinfect it with 75% ethanol in an ultra-clean platform for 30 seconds, and then use 0.1% HgCl 2 Disinfect for 5 minutes, and finally rinse 3 times with sterile water.

[0036] 2. Place the sterilized stem tip of Luo Han Guo in MS medium for light cultivation to induce germination.

[0037] 3. After 15 days of germination, cut the shoot tips of the shoots, induce with 0.2% colchicine for 48 hours, and continue to culture on MS medium after induction.

[0038] 4. Cut out one leaf of diploid Luo Han Guo and 30 days of colchicine-induced tender shoots, conduct cell flow cytometry analysis and repeat 3 times, all identified as tetraploid strains continue to culture, diploid, quadruple The result of flow cytometry of body Luo Han Guo cell...

Embodiment 2 4

[0074] Example 2. Cultivation of Seedless Monk Fruit from Tetraploid Female Parents

[0075] Take the stem tip of the female Luo Han Guo plant with a good phenotype in the field, wash it with running water for 3 hours, disinfect it with 75% ethanol for 30 seconds in the ultra-clean bench, and then use 0.1% HgCl 2 Disinfect for 5 minutes and rinse with sterile water 5 times. The sterilized shoot tip of Luo Han Guo was placed in MS medium for light cultivation to induce germination. After the buds grow to 21 days, cut the shoot tips of the shoots, induce with 0.2% colchicine for 36 hours, and continue to culture on MS medium after induction. Cut out 2 leaves of shoots induced by colchicine for about 30 days, carry out cell flow cytometry analysis and repeat more than 3 times and continue to culture the strains identified as tetraploid. The flow cytometry detection technique is the same as that in Example 1.

[0076] Hybridize the obtained tetraploid female plant with the diplo...

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Abstract

The invention provides a technology for cultivating seedless momordica grosvenori by combining plant tissue culture, polyploid induction and genetic breeding hybridization technologies, and belongs to the field of a modern biological technology. The momordica grosvenori obtained by the technology is great in fruit shape, high in mogroside content and high in whole fruit utilization rate, so that the current situation of a momordica grosvenori planting industry is completely changed, and the difficulty that the cost of mogroside is high and the mogroside is difficult to widely apply can be solved; and the application of the momordica grosvenori in industries of foods, beverages, medicines and the like is promoted and the development of a momordica grosvenori industry is accelerated.

Description

technical field [0001] The invention combines plant tissue culture, polyploid induction and genetic breeding hybridization technology to cultivate seedless Luo Han Guo, which belongs to the field of modern biotechnology. Background technique [0002] Mangosteen [Siraitia grosvenorii (Swingle) C. Jeffrey] belongs to Cucurbitaceae (Cucurbitaceae) Siraitia, is a perennial herbaceous vine, dioecious, and is a unique economic and medicinal plant in my country. Mogroside belongs to cucurbitane triterpenoids. Mogroside V is one of the strongest sweet substances in the world. Its sweetness is 2 times that of stevioside, 6 times that of glycyrrhizin, and 300 times that of sucrose; it does not contain calories, Without any toxic and side effects, it is suitable for all people, especially special groups such as diabetes, obesity and high blood pressure. It is an internationally recognized natural functional sweetener with the best comprehensive properties. [0003] Mogroside exists i...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/08A01H4/00A01H5/08
Inventor 张会新张继贤王小英
Owner 怀化兴科创生物技术有限公司
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