Technology for directly cultivating seedless momordica grosvenori by utilizing tetraploid momordica grosvenori female plants
A seedless Luo Han and Luo Han Guo technology, applied in the application, plant genetic improvement, angiosperm/flowering plants and other directions, can solve the problems of inability to apply, the fruit of Luo Han Guo becomes smaller and shorter, and achieves shortening of the breeding cycle and the utilization rate of the whole fruit. Increase, overcome severely diminished effects
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Embodiment 1 4
[0034] Example 1. Cultivation of high-sweetness seedless Luo Han Guo derived from tetraploid female parent
[0035] 1. Take the stem tip of the female Luo Han Guo plant with a good phenotype in the field, wash it with running water for 2 hours, disinfect it with 75% ethanol in an ultra-clean platform for 30 seconds, and then use 0.1% HgCl 2 Disinfect for 5 minutes, and finally rinse 3 times with sterile water.
[0036] 2. Place the sterilized stem tip of Luo Han Guo in MS medium for light cultivation to induce germination.
[0037] 3. After 15 days of germination, cut the shoot tips of the shoots, induce with 0.2% colchicine for 48 hours, and continue to culture on MS medium after induction.
[0038] 4. Cut out one leaf of diploid Luo Han Guo and 30 days of colchicine-induced tender shoots, conduct cell flow cytometry analysis and repeat 3 times, all identified as tetraploid strains continue to culture, diploid, quadruple The result of flow cytometry of body Luo Han Guo cell...
Embodiment 2 4
[0074] Example 2. Cultivation of Seedless Monk Fruit from Tetraploid Female Parents
[0075] Take the stem tip of the female Luo Han Guo plant with a good phenotype in the field, wash it with running water for 3 hours, disinfect it with 75% ethanol for 30 seconds in the ultra-clean bench, and then use 0.1% HgCl 2 Disinfect for 5 minutes and rinse with sterile water 5 times. The sterilized shoot tip of Luo Han Guo was placed in MS medium for light cultivation to induce germination. After the buds grow to 21 days, cut the shoot tips of the shoots, induce with 0.2% colchicine for 36 hours, and continue to culture on MS medium after induction. Cut out 2 leaves of shoots induced by colchicine for about 30 days, carry out cell flow cytometry analysis and repeat more than 3 times and continue to culture the strains identified as tetraploid. The flow cytometry detection technique is the same as that in Example 1.
[0076] Hybridize the obtained tetraploid female plant with the diplo...
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