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Aspergillus niger liquid fermentation assisted method for extraction of red date pigment

A technology of liquid fermentation and Aspergillus niger, which is applied in the field of fruit pigment extraction and the use of microbial technology to extract fruit pigments. It can solve the problems of no microbial fermentation extraction of jujube pigment and no reports of jujube red pigment extraction, etc., to achieve anti-oxidation Similar ability, improved extraction efficiency, and significant dose-effect relationship

Inactive Publication Date: 2014-09-10
新疆刀郎枣业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, in the prior art, reports on the extraction of red pigment or jujube pigment are mainly concentrated on traditional chemical extraction methods, biological enzymatic extraction and other methods. There is no report on the extraction of jujube pigment by microbial fermentation, let alone the extraction of jujube red pigment.

Method used

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  • Aspergillus niger liquid fermentation assisted method for extraction of red date pigment
  • Aspergillus niger liquid fermentation assisted method for extraction of red date pigment
  • Aspergillus niger liquid fermentation assisted method for extraction of red date pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Utilize Aspergillus niger liquid fermentation to extract jujube pigment

[0039] For details, see the attached figure 1 , % is calculated according to weight percentage, and the method for extracting jujube pigment by liquid fermentation of Aspergillus niger is as follows.

[0040] (1) Sieving: Pass the crushed jujube skin through a 40-mesh sieve.

[0041] (2) Liquid fermentation: The jujube peel powder crushed and sieved in the above steps is used as a fixed carbon source, sodium nitrate is used as a nitrogen source, and the carbon-to-nitrogen ratio is 5:1 by weight. The addition amount of skin powder was 2%, Tween-80 was 0.02%, and the addition amount of Aspergillus niger liquid strain was 10%. After fermentation at 30°C for 5 days, it was sterilized at 121°C for 15 minutes.

[0042] (3) Drying: Dry in an oven at 80°C for 1 hour.

[0043] (4) Extraction: Use 0.8% NaOH solution as the extractant, and the extraction temperature is 80°C. The dry material ...

Embodiment 2

[0046] Embodiment two: traditionally adopted chemical method extracts jujube pigment

[0047] For details, see the attached figure 2 , the technical points of extracting red jujube pigment by the traditional chemical method are as follows:

[0048] The pulverized jujube skin is passed through a mesh sieve, and after extraction and centrifugation, the extract is centrifuged at a speed of 4000r / min, and the supernatant is taken. The specific preparation process parameters are well known in the art, and the same technical steps as in Example 1 can be used. Basic parameters.

Embodiment 3

[0049] Example 3: Optimization of Aspergillus niger liquid fermentation method to help extract jujube pigment

[0050] 1. The effect of nitrogen source on the extraction yield of jujube pigment:

[0051] Select urea, peptone, sodium nitrate, yeast extract, and ammonium chloride as nitrogen sources, respectively, and add them to the liquid fermentation medium at a carbon-to-nitrogen ratio of 6:1 to investigate the effects of different nitrogen sources on the extraction yield of jujube pigments. See the attached image 3 .

[0052] In order to consume the plant cellulose in the jujube peel and change the permeability of the cell wall, the red jujube peel is directly used as a fixed carbon source to avoid the influence of other substances as carbon sources on the extraction yield of the red jujube pigment. Sodium nitrate is the best carbon source for the fermentation method of Aspergillus niger strain to help extract the pigment of jujube.

[0053] 2. The effect of carbon-nit...

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Abstract

The invention discloses a method for using Aspergillus niger liquid fermentation to assist in extracting jujube pigment. Using Aspergillus niger as the fermentation strain, jujube skin powder as the fixed carbon source, sodium nitrate as the nitrogen source, the carbon-to-nitrogen ratio was 5:1, the addition amount of red jujube skin powder was 2%, and Tween-80 was 0.02%. Aspergillus liquid strains were added in an amount of 10%, fermented at 30°C for 5 days, sterilized at 121°C for 15 minutes, dried in an oven at 80°C for 1 hour, and 0.8% NaOH solution was used as the extractant, and the extraction temperature was 80°C. The dry material and extractant are 1:20 (g / ml) according to the ratio of solid to liquid, the extraction time is 4 hours, and the number of stages is 3. The extraction solution is centrifuged at 4000r / min for 15 minutes, and the supernatant is taken. Compared with the traditional chemical extraction method, the product yield of the method provided by the application is increased by 2.11%, good technical effect is obtained, and it has extensive practical value.

Description

technical field [0001] The invention belongs to the technical field of extracting fruit pigments, in particular, the invention relates to the technical field of extracting fruit pigments by means of microbial technology. Background technique [0002] Natural pigments are important additives in the fields of food, health care and cosmetics. Compared with synthetic pigments, its biggest advantage is higher safety. And most of them contain high nutritional value and pharmacological activity. Jujube is distributed all over the north and south of my country. The yield is large and rich in natural red pigment. Extracting red pigment from jujube or a large amount of discarded jujube peels during processing not only comprehensively utilizes jujube resources, but also has great benefits for environmental protection. Studies have shown that jujube red pigment has a good stabilizing effect on oxidizing agents and reducing agents, and is an ideal food additive and medicinal raw mate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00
Inventor 敬思群王侠房芳
Owner 新疆刀郎枣业有限公司