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Product for performing assisted prediction on postoperative survival time length of esophageal squamous carcinoma patient

A technology for esophageal squamous cell carcinoma and survival time, which can be used in medical preparations containing active ingredients, drug combinations, anti-tumor drugs, etc. It can solve the problem of not finding miRNA classifiers and achieve the effect of improving the disease

Active Publication Date: 2015-02-25
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no classifier of miRNA composition that can stably predict the prognosis of patients with esophageal squamous cell carcinoma has been found

Method used

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  • Product for performing assisted prediction on postoperative survival time length of esophageal squamous carcinoma patient
  • Product for performing assisted prediction on postoperative survival time length of esophageal squamous carcinoma patient
  • Product for performing assisted prediction on postoperative survival time length of esophageal squamous carcinoma patient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, establishment of the inventive method

[0031] 1. Obtaining specimens and survival information

[0032] 119 patients with primary esophageal squamous cell carcinoma who had undergone esophageal squamous cell carcinoma resection in the Cancer Institute of the Chinese Academy of Medical Sciences and adjacent normal tissues (frozen in liquid nitrogen and then transferred to a -80°C refrigerator) were used as specimens; all patients None of them received radiotherapy or chemotherapy before operation; the survival information of the patients was obtained through telephone follow-up. Informed consent was obtained from each patient.

[0033] For each specimen, a portion was reserved for paraffin embedding, sectioned, and routinely stained with H&E for pathological examination. The pathological features of each specimen were independently tested by two case physicians and were consistent, and the proportion of cancer cells in all tumor specimens was above 60%. ...

Embodiment 2

[0073] Embodiment 2, the application of the inventive method

[0074] The cancer tissues of another 47 esophageal squamous cell carcinoma patients (patients to be tested) who underwent esophageal squamous cell carcinoma resection in the Cancer Institute of the Chinese Academy of Medical Sciences were collected, and the postoperative survival time of each patient to be tested was predicted according to the following steps:

[0075] 1. Total RNA extraction and reverse transcription

[0076] According to the method in step 2 of Example 1, the total RNA of the cancer tissues of each patient to be tested was extracted respectively.

[0077] 2. Detection of miRNA expression

[0078] The expression levels of 178 miRNAs shown in Table 2 in the cancer tissue of the patient to be tested were detected according to the method in step 3 of Example 1.

[0079] 3. Data normalization processing

[0080] Rank the above 178 miRNA expression data of each patient's cancer tissue obtained in st...

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Abstract

The invention discloses a product for performing assisted prediction on postoperative survival time length of an esophageal squamous carcinoma patient. The product comprises substances for detecting 178 miRNA expression quantity substances comprising hsa-miR-218-5p, hsa-miR-142-3p, hsa-miR-150-5p and hsa-miR-205-5p, and records a carrier with the diagnostic criteria and functional expressions as follows: if 0.94dig is less than dip, the postoperative survival time of the esophageal squamous carcinoma patient to be tested is more than 5 years, and if the 0.94dig is more than or equal to dip, the postoperative survival time of the esophageal squamous carcinoma patient to be tested is less than 5 years. After the result of the postoperative survival time of the esophageal squamous carcinoma patient is predicted by using the product, more accurate and effective treatment is adopted, and the patient condition can be effectively improved. In addition, the medicine which influences the expression quantities of hsa-miR-218-5p, hsa-miR-142-3p, hsa-miR-150-5p and hsa-miR-205-5p can be used for treating the esophageal squamous carcinoma.

Description

technical field [0001] The invention relates to a medical diagnosis product, in particular to a product for assisting in predicting postoperative survival time of patients with esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is the eighth most common malignant tumor in the world, and its death rate ranks sixth among all cancers. There are two histological types of esophageal cancer: adenocarcinoma and squamous cell carcinoma. At present, TNM staging provides an important basis for clinical prediction of the survival of patients with esophageal squamous cell carcinoma. However, clinicians have found that the prognosis of patients with the same clinical stage and similar treatment strategies may be quite different. This suggests that the current clinical stage mainly based on tumor anatomical characteristics is not accurate enough as a prognostic indicator. Research over the past decade has revealed the heterogeneity of tumors. Different...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A61K45/00A61K45/06A61P35/00
Inventor 赫捷陈照丽李嘉根
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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