Oyster polysaccharide gel formulation capable of playing protection role in immunological liver injury

A technology of immune liver injury and protective effect, applied in the field of research and development of pharmaceutical products, can solve the problem of insignificant curative effect and other problems

Inactive Publication Date: 2013-08-28
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, the curative effect of the high-dose oyster polysaccharide group is more obvious, but the curative effect of the low-dose oyster polysaccharide group is not significant, and it shows that the curative effect can be enhanced with the increase of the dose within the test dose range

Method used

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  • Oyster polysaccharide gel formulation capable of playing protection role in immunological liver injury
  • Oyster polysaccharide gel formulation capable of playing protection role in immunological liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Step (1): Select 10 kg of fresh oysters, shell the oysters, wash the oyster meat, remove impurities, drain, beat, add bromelain to the homogenate, the amount of enzyme added is 3% of the homogenate, and adjust with 10% sodium carbonate The pH is 8.0, enzymolysis at 65°C for 4 hours; then adjust the pH to 8.5 and add alkaline protease, and enzymolysis at 55°C for 3 hours. Place the extract at 90°C for 20 minutes, inactivate bromelain and alkaline protease, cool, centrifuge at high speed and low temperature (10000r / min) for 30 minutes, take the supernatant to obtain about 290 g of oyster crude polysaccharide, freeze-dry about 290 g, and set aside;

[0013] Step (2): Dissolve the freeze-dried powder in step (1) with 10 times of water, let it stand still, centrifuge to remove insoluble matter, filter with filter paper, slowly add 2 times of 95% ethanol to the filtrate, and overnight at 4°C , centrifuged, ethanol was recovered from the supernatant, the precipitate was washed...

Embodiment 2

[0017] Step (1): Select 20 kg of fresh oysters, shell the oysters, wash the oyster meat, remove impurities, drain, beat, add bromelain to the homogenate, the amount of enzyme added is 2% of the homogenate, and adjust with 10% sodium carbonate The pH is 7.5, enzymolysis at 60°C for 3h; then adjust the pH to 8.5 and add alkaline protease, and enzymolysis at 55°C for 4h. Place the extract at 90°C for 10 minutes to inactivate bromelain and alkaline protease, cool, centrifuge at high speed and low temperature (8500r / min) for 30 minutes, take the supernatant to obtain about 550 g of oyster crude polysaccharide, freeze-dry about 550 g, and set aside;

[0018] Step (2): Dissolve the freeze-dried powder in step (1) with 20 times of water, let it stand still, centrifuge to remove insoluble matter, filter with filter paper, slowly add 3 times of 95% ethanol to the filtrate, and keep overnight at 4°C , centrifuged, ethanol was recovered from the supernatant, the precipitate was washed wit...

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PUM

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Abstract

The invention belongs to the technical field of research and development of medicine products, and relates to a biotechnological product formulation capable of playing a protection role in immunological liver injury. The method comprises the following steps of: shelling oysters; cleaning oyster meat; removing impurities; draining; beating; carrying out enzymolysis; extracting effective components, and further purifying to prepare the gel formulation; bottling; and sealing for use when needed. The product disclosed by the invention plays the protection role in the liver and a conditioning role in a liver function and is favorable to the industrialized production.

Description

technical field [0001] The invention belongs to the technical field of research and development of pharmaceutical products, and specifically relates to an oyster polysaccharide gel preparation that has a protective effect on immune liver damage. The product of the invention has a protective effect on the liver and has a conditioning effect on liver function. The product of the invention is beneficial to industrialization Production. Background technique [0002] Immune liver injury (including autoimmune hepatitis) is a kind of hepatitis that cannot be cured naturally due to the decreased immune tolerance of the patient's liver. Regarding the pathogenesis of autoimmune hepatitis, the latest view holds that the body, under the action of susceptibility genes, is induced by environmental factors, drugs or infectious agents to develop the disease. Loss of immune tolerance, leading to liver damage. The immune liver injury induced by BCG combined with lipopolysaccharide in mice i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K31/715A61P1/16A61K35/56A61K35/618
Inventor 张莉刘叶舟田振华卢学敏
Owner SHANDONG UNIV
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