Application of DNA (Deoxyribonucleic Acid)-PKcs in preparation of medicine for changing drug resistance of tumor cells on MTX (Methotrexate)
A tumor cell, MTX technology, applied in the field of biomedicine, can solve problems such as genome instability
Active Publication Date: 2013-09-18
HARBIN MEDICAL UNIVERSITY
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Although there are no reports of DNA-PKcs causing gene amplification, gene a
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The invention relates to application of DNA (Deoxyribonucleic Acid)-PKcs in preparation of a medicine for influencing dihydrofolate reductase (DHFR) gene amplification and changing the drug resistance of tumor cells on MTX (Methotrexate) and belongs to biological medicines. The application comprises the following steps: establishing an MTX drug-resistant cell evolution model, selecting low-concentration drug-resistant (HSRs) and high-concentration drug-resistant (DMs) cells as research objects, detecting the gene amplification degree and form in the parent cells and drug-resistant cells, the DNA damage conditions and DNA-PKcs expression conditions, establishing clone which stably interferes the DNA-PKcs in the MTX drug-resistant cells aiming at the core protein DNA-PKcs in the NHEJ path, and detecting the amplification degree and form of DHFR genes in stable interference clone, the cell growth and apoptosis and change of the drug resistance.
Description
technical field [0001] The present invention relates to the application of DNA-PKcs in the preparation of drugs for changing the drug resistance of tumor cells to MTX, in particular to the application of DNA-PKcs in the preparation of drugs for affecting the amplification of DHFR genes and changing the drug resistance of tumor cells to MTX. Biomedical field. Background technique [0002] Methotrexate (MTX) is the first effective anti-metabolite in the treatment of malignant tumors. It is currently the most widely used folic acid antagonist in tumor treatment. It can competitively bind to dihydrofolate reductase DHFR) inhibits nucleotide metabolism, thereby blocking DNA synthesis and specifically killing proliferating cells. Although MTX has become a commonly used drug for tumor treatment, long-term use will lead to drug resistance, which will seriously affect the efficacy of the drug, so how to overcome MTX drug resistance is the main problem to improve the efficiency of it...
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Login to view more IPC IPC(8): A61K38/45A61P35/00A61K31/519
Inventor 傅松滨孟祥宁朱静李春香金焰孙文靖于旸
Owner HARBIN MEDICAL UNIVERSITY
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