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Application of labdane diterpene glycoside compounds in rubus chingii Hu as medicine

A technology of raspberry palmate, diterpene glycosides, applied in the field of medicine

Inactive Publication Date: 2013-09-25
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But so far, there is no relevant report about the activity of helianthane-type diterpene glycosides in raspberry palmate to prevent and treat chronic nephritis, kidney-yang deficiency, osteoporosis and prostatitis

Method used

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  • Application of labdane diterpene glycoside compounds in rubus chingii Hu as medicine
  • Application of labdane diterpene glycoside compounds in rubus chingii Hu as medicine
  • Application of labdane diterpene glycoside compounds in rubus chingii Hu as medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, preparation raspberry extract

[0033] (1) extract

[0034] 50kg of immature fruit of Raspberry palmis, with 250L of hydrous ethanol 250L of 70% percolates to obtain extracting solution by conventional method, centrifugal after extracting solution decompression and concentration, get supernatant for subsequent use;

[0035] (2) Purification

[0036] The above supernatant was subjected to polystyrene-type porous adsorption resin adsorption, and eluted with water to remove impurities, then eluted with 80L of 60% aqueous alcohol, and the eluate containing helianthin-type diterpene glycosides was collected.

[0037] (3) Concentration and drying

[0038] The eluate was concentrated under reduced pressure and dried to obtain 6.5 kg of raspberry extract.

Embodiment 2

[0039] Embodiment 2, preparation raspberry extract

[0040] (1) extract

[0041] 50kg of immature fruit of Raspberry palmis, with 250L of hydrous ethanol 250L of 50% percolation to obtain extracting solution by conventional method, centrifugal after extracting solution decompression and concentrating, get supernatant for subsequent use;

[0042] (2) Purification

[0043] The above supernatant was subjected to polystyrene type porous adsorption resin adsorption, and after removing impurities by elution with water, it was eluted with 100L of 50% aqueous alcohol, and the eluate containing helianthin-type diterpene glycosides was collected.

[0044] (3) Concentration and drying

[0045] The eluate was concentrated under reduced pressure and dried to obtain 7.3 kg of raspberry extract.

Embodiment 3

[0046] Embodiment 3, the preparation of the helicanane type diterpene glycoside compound contained in the raspberry extract

[0047] Take 6.5 kg of the raspberry extract prepared in Example 1, suspend it in water, and carry out adsorption with polystyrene-type porous adsorption resin (D101, Tianjin Nankai University Chemical Plant), and then elute with water, 60% ethanol, and 95% ethanol respectively, Collect and concentrate 60% eluate to obtain 60% ethanol fraction. The 60% ethanol part was routinely subjected to silica gel (particle size 200-300 mesh, Shandong Yantai Zhifu Huangwu Silica Gel Development and Experimental Factory) column chromatography, followed by ethyl acetate-ethanol (16:2:1, 8:2:1 and 5:2:1) gradient elution and monitored by TLC (silica plate for HSGF 254 , particle size 8±2μm, Shandong Yantai Zhifu Huangwu Silica Gel Development and Experimental Factory), the developer is ethyl acetate: ethanol: water 10:1:0.5-4:2:1. details as follows:

[0048] Elute ...

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Abstract

The invention relates to the field of medical technology and provides an application of labdane diterpene glycoside compounds in rubus chingii Hu in preparing a medicine for preventing or treating chronic nephritis, kidney-yang deficiency, osteoporosis and prostatitis. The general formula of the chemical structure of the labdane diterpene glycosides in rubus chingii Hu is shown by the formula (I) in the specification. According to in-vivo animal experiments, the compounds provided by the invention have activity in preventing and treating chronic nephritis, kidney-yang deficiency, osteoporosis and prostatitis, wherein the curative effect of raspberry glycoside-F1-7 is better.

Description

technical field [0001] The invention relates to the technical field of medicine, specifically refers to the use of helicanoid diterpene glycosides contained in Rubus chingii Hu in the preparation of prevention and treatment of chronic nephritis, kidney yang deficiency, osteoporosis and prostatitis application in medicine. Background technique [0002] Palm leaf raspberry (Rubus chingii Hu), also known as East China raspberry, is a plant of the genus Rubus in the family Rosaceae. Its dried immature fruit is used as medicine and is called raspberry. . [0003] In order to develop and utilize this plant, the inventors have carried out a systematic study on the chemical components of this plant, and isolated a series of helicanoid diterpene glycosides from it, see literature: Nan Sun, Yan Wang, Yun Liu, Mei-Li Guo, and Jun Yin, A NEW ent-LABDANE DITERPENE SAPONIN FROM THE FRUITS OF Rubus chingii, Chemistry of Natural Compounds, Vol.49, No.1, March, 2013, 49-53. [0004] But s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7032A61K31/704A61P13/12A61P19/10A61P13/08A61P29/00
Inventor 郭美丽何建明高越
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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