Method for detecting canine rabies virus antibody and detection kit

A rabies virus and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to use canine serum or plasma, and the sensitivity and accuracy are not as good as enzyme-linked immunosorbent assays, and achieve high accuracy.

Inactive Publication Date: 2013-09-25
ZHENGZHOU ZHONGDAO BIOTECHNOLOGY CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

Anti-rabies virus IgG antibody ELISA detection kit with application number 201010257264 for Chinese patent, this kit is a detection of whether there is antibody in human serum or plasma, and cannot be used for canine serum or plasma
The Chinese patent with the application number 201010197930 discloses a colloidal gold test paper card for detecting rabies virus antibodies in dogs and cats and its preparation method and application. Immunization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Canine rabies virus antibody (IgG) detection kit consists of two parts: coated plate and reagent reaction system, including rabies virus antigen coated reaction plate, standard, positive control serum, negative control serum, washing solution (20×), sample Diluent, enzyme-labeled conjugate, chromogen A, chromogen B, and stop solution.

[0035] (1) Rabies virus antigen-coated reaction plate: the coating volume is 100ul / well of rabies virus antigen, the antigen concentration is 1mg / mL, and the specification is 96 wells / block;

[0036] (2) Standard product: rabies antibody standard product, the potency is 0.5IU / mL, and the specification is 0.5mL / bottle;

[0037] (3) Positive control serum: 1mL canine positive serum, 100mL 0.01mol / L PBS (pH7.2), 1mL / bottle;

[0038] (4) Negative control serum: Canine negative serum 1mL, 0.01mol / L PBS (pH7.2) 100mL, specification 1mL / bottle;

[0039] (5) Lotion (20×): Each 1000ml contains 4g potassium dihydrogen phosphate, 58g disodium hyd...

Embodiment 2

[0046] The canine rabies virus antibody (IgG) detection kit was used to detect 218 dog sera, and the neutralization method was used for comparative experiments.

[0047] 1. Detection method

[0048] (1) Numbering: 1 blank control well; 3 standard wells; 1 negative control well; 1 positive control well; the rest are sample wells to be tested, numbered 1-218.

[0049] (2) Add sample diluent: use a sampler to add sample diluent to the standard wells and sample wells of the coated plate, 100ul per well; do not add sample diluent to the blank control wells, negative control wells and positive control wells.

[0050] (3) Adding samples: add 10ul of the standard substance to the standard well, add 10ul of the sample to be tested in each well of the sample to be tested, add 100ul of negative control serum to the negative control well, add 100ul of positive control serum to the positive control well; do not add samples to the blank control well .

[0051] (4) Incubation (1): Mix well...

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Abstract

The invention provides a method for detecting canine rabies virus antibody (IgG) and a detection kit. The kit is composed of a coated plate and a reagent reaction system, and comprises a rabies virus antigen-coated reaction plate, a standard substance, positive contrast serum, a washing lotion (20X), a sample weak solution, an enzyme-marked combination substance, a developer A, a developer B and a stopping solution. The method is characterized in that the method uses an ELISA method to determine that the content of the canine rabies virus antibody (IgG) reaches a absorbance corresponding to a protective level by detecting the standard substance of the kit, and by compared the absorbance of the sample to be detected with the absorbance of the standard substance, the content of the canine rabies virus antibody (IgG) contained in the sample to be detected is judged whether to reach an immunization protective level. The method and the kit can simultaneously detect a lot of samples, and a detection result is remarkably with a result by a neutralization experiment, has high accuracy degree, is suitable for monitoring an immunization inoculation effect and determining an individual immunization state, and can be used for investigating animal eqpidemic diseases.

Description

technical field [0001] The invention relates to a detection method of canine rabies virus antibody (IgG), especially provides a canine rabies virus antibody (IgG) detection kit, which can be used for monitoring the effect of immunization and determining the immune status of individuals, belonging to animal epidemics detection field. Background technique [0002] Rabies (rabies), also known as hydrophobia, is a zoonotic acute infectious disease of the central nervous system caused by rabies virus. The disease is common in dogs, wolves, cats and other carnivores. Infected by the bite of a sick animal, once the disease occurs, the mortality rate is almost 100%. According to the latest national statutory report infectious disease epidemic situation announced by the Ministry of Health, rabies still ranks first in the number of deaths reported by infectious diseases. In recent years, due to the gradual increase of dog breeding, the incidence rate has a rising trend. Therefore, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 曾小宇张如民路纪琪任宝红王振龙赵林萍王思敏魏卓
Owner ZHENGZHOU ZHONGDAO BIOTECHNOLOGY CO LTD
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