Method for detecting canine rabies virus antibody and detection kit
A rabies virus and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to use canine serum or plasma, and the sensitivity and accuracy are not as good as enzyme-linked immunosorbent assays, and achieve high accuracy.
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Embodiment 1
[0034] Canine rabies virus antibody (IgG) detection kit consists of two parts: coated plate and reagent reaction system, including rabies virus antigen coated reaction plate, standard, positive control serum, negative control serum, washing solution (20×), sample Diluent, enzyme-labeled conjugate, chromogen A, chromogen B, and stop solution.
[0035] (1) Rabies virus antigen-coated reaction plate: the coating volume is 100ul / well of rabies virus antigen, the antigen concentration is 1mg / mL, and the specification is 96 wells / block;
[0036] (2) Standard product: rabies antibody standard product, the potency is 0.5IU / mL, and the specification is 0.5mL / bottle;
[0037] (3) Positive control serum: 1mL canine positive serum, 100mL 0.01mol / L PBS (pH7.2), 1mL / bottle;
[0038] (4) Negative control serum: Canine negative serum 1mL, 0.01mol / L PBS (pH7.2) 100mL, specification 1mL / bottle;
[0039] (5) Lotion (20×): Each 1000ml contains 4g potassium dihydrogen phosphate, 58g disodium hyd...
Embodiment 2
[0046] The canine rabies virus antibody (IgG) detection kit was used to detect 218 dog sera, and the neutralization method was used for comparative experiments.
[0047] 1. Detection method
[0048] (1) Numbering: 1 blank control well; 3 standard wells; 1 negative control well; 1 positive control well; the rest are sample wells to be tested, numbered 1-218.
[0049] (2) Add sample diluent: use a sampler to add sample diluent to the standard wells and sample wells of the coated plate, 100ul per well; do not add sample diluent to the blank control wells, negative control wells and positive control wells.
[0050] (3) Adding samples: add 10ul of the standard substance to the standard well, add 10ul of the sample to be tested in each well of the sample to be tested, add 100ul of negative control serum to the negative control well, add 100ul of positive control serum to the positive control well; do not add samples to the blank control well .
[0051] (4) Incubation (1): Mix well...
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