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Application of Chukrasone A in preparation of medicaments for treating lung cancer

A drug and lung cancer technology, applied in the field of anti-tumor drugs, can solve the problem of damage to normal tissues and cells, and achieve the effect of strong cytotoxicity and prominent substantive characteristics

Active Publication Date: 2013-12-11
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Malignant tumors are diseases that seriously threaten human life and health. Chemotherapy drugs commonly used in clinical practice can greatly damage normal tissues and cells while killing tumor cells, which limits its application.

Method used

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  • Application of Chukrasone A in preparation of medicaments for treating lung cancer
  • Application of Chukrasone A in preparation of medicaments for treating lung cancer
  • Application of Chukrasone A in preparation of medicaments for treating lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: the preparation of the compound Chukrasone A tablet involved in the present invention:

[0013] Take 20 grams of compound Chukrasone A, add 180 grams of conventional excipients for tablet preparation, mix well, and make 1000 tablets with a conventional tablet press.

Embodiment 2

[0014] Embodiment 2: the preparation of the compound Chukrasone A capsule involved in the present invention:

[0015] Get 20 grams of compound Chukrasone A, add conventional auxiliary materials for preparing capsules such as 180 grams of starch, mix well, and pack into capsules to make 1000 tablets.

[0016] The following pharmacodynamic experiments will further illustrate its drug activity.

experiment example

[0017] Experimental example: the inhibitory effect of Chukrasone A on the proliferation of lung cancer cells cultured in vitro and the effect on human normal cells

[0018] Lung cancer cells in the logarithmic growth phase (non-small cell lung cancer cells A 549 , large cell lung cancer cells NCI-H460, lung squamous cell carcinoma cells HTB-8 and human lung squamous cell carcinoma cells QG-5) and human normal liver cells L02 were inoculated on 96-well culture plates, and the seeding volume per well was 3000-4000 Cells / 100uL and 6000 cells / 100uL; aseptically collect 15 mL of venous blood from healthy blood donors, anticoagulate with heparin, separate mononuclear cells by Ficoll density gradient centrifugation, prepare single cell suspension with RPMI1640 containing 10% fetal bovine serum, Adjust the cell concentration to 2×10 5 cells / mL, inoculated in 96-well culture plate, 100 uL per well. After 24 hours of cell culture on the above-mentioned culture plates, the experimental...

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PUM

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Abstract

The invention discloses application of Chukrasone A in preparation of medicaments for treating lung cancer. The compound shows excellent activity for resisting lung cancer, can be used for preparing medicaments for resisting lung cancer, and belongs to the technical field of novel application of medicaments. Chukrasone A can remarkably inhibit in vitro cultured non-small cell lung cancer cells A549, large cell lung cancer cells NCI-H460, squamous cell lung carcinoma cells HTB-8 and human lung epidermoid carcinoma QG-5, but hardly has inhibition effects on human normal hepatocytes LO2 and peripheral blood lymphocytes, and shows a remarkable selectivity. The application of Chukrasone A in preparation of medicaments for treating lung caner is disclosed for the first time, and the frame type belongs to a brand-new frame type, so that the inhibition activity of the Chukrasone A to lung cancer cells is unexpectedly strong.

Description

technical field [0001] The invention relates to the field of antitumor drugs, in particular to the application of Chukrasone A in the preparation of antitumor drugs. Background technique [0002] Malignant tumors are diseases that seriously threaten human life and health. Chemotherapy drugs commonly used in clinical practice can greatly damage normal tissues and cells while killing tumor cells, which limits its application. It has become the direction and urgent task of current anticancer drug research to find highly efficient and low toxic drugs for treating tumors. Finding high-efficiency, low-toxic anticancer drugs has been considered an effective way. [0003] The present invention has confirmed by experiments that Chukrasone A has the effect on non-small cell lung cancer cell A cultured in vitro. 549 , large cell lung cancer cell line NCI-H460, lung squamous cell carcinoma cell line HTB-8 and human lung squamous epithelial cell line QG-5 have significant inhibitory ef...

Claims

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Application Information

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IPC IPC(8): A61K31/585A61P35/00
Inventor 刘洋黄蓉王慧张广江春平吴俊华
Owner NANJING UNIV
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