Anther culture solution

A technology for culture medium and anther, applied in the direction of plant cells, etc., can solve the problems of inability to meet nutritional needs, difficulty in meeting nutritional and physiological needs of plant cells, and inappropriate amounts and proportions of inorganic nutrients.

Inactive Publication Date: 2013-12-11
NANJING NIANJI REFRIGERATION FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing anther culture solution cannot meet the nutritional needs of each stage in the anther culture process, especially the amount and proportion of inorganic nutrients are not suitable, and it is difficult to meet the nutritional and physiological needs of plant cells at each stage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] An anther culture solution, the culture solution is composed of inorganic nutrients, carbon sources, vitamins, growth regulators and organic appendages, the carrier is water,

[0022] Among them, inorganic nutrients are made according to the following ratio: KNO 3 : 150~200mg / L, NH 4 NO 3 : 50~100mg / L, KH 2 PO 4 : 350~500mg / L, K 2 SO 4 : 100~150mg / L, CaCl 2 : 500~650mg / L, NaCl: 50~80mg / L, ZnCl 2 : 0.5~1.0mg / L, CuSO 4 ·H 2 O: 0.05~0.08mg / L, MnSO 4 ·H 2 O: 0.8~1.5mg / L, FeSO 4 : 1.0~1.5mg / L;

[0023] Wherein, the carbon source is sucrose or glucose;

[0024] Wherein, the vitamins are vitamin B1, vitamin B6 and vitamin H;

[0025] Wherein, the growth regulating substance is made of the following ratio: 1~2 mg / L zeatin and 2~3 mg / L 6-benzyl adenine;

[0026] Wherein, the organic additives are yeast extract and amino acid.

[0027] Wherein, the pH value of the culture solution is 5.5-6.5.

[0028] Wherein, the EC value of the culture solution is 1.0-1.5 ms / ...

Embodiment 2

[0041] The preparation method of above-mentioned anther culture fluid comprises the steps:

[0042] Step 1, configure 100L anther culture solution, weigh 75g KNO 3 Dissolve in 5L distilled water to obtain mother liquor a; weigh 25 g NH 4 NO 3 Dissolve in 5L distilled water to obtain mother liquor b; weigh 175g KH 2 PO 4 Dissolve in 5L distilled water to obtain mother liquor c; weigh 50g K 2 SO 4 Dissolve in 5L distilled water to obtain mother liquor d; weigh 250g CaCl 2 Dissolve in 5L distilled water to obtain mother liquid e; weigh 2 5g NaCl and dissolve in 5L distilled water to obtain mother liquid f; weigh 0.2 5g ZnCl respectively 2 , 0.025g CuSO 4 ·H 2 O, 0.4gMnSO 4·H 2 O was dissolved in 5L distilled water to obtain the mother liquor g; weigh FeSO 4 ·7H 2 05g and disodium edetate 7.45g were dissolved in 5L distilled water to obtain mother liquor h;

[0043] Step 2, weigh 100g sucrose, 0.2gL-asparagine, 0.5gL-cystine, 0.6gL-alanine, 0.3g vitamin B1, 0.7g vita...

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PUM

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Abstract

The invention discloses an anther culture solution which comprises an inorganic nutrient, a carbon source, vitamin, a growth regulating substance and an organic additive, and takes water as a carrier. According to the anther culture solution, a large number of nutrient elements and microelements are added to the culture solution according to a nutrient requirement of an anther culture process; particularly adding of the growth regulating substance, a yeast extract and amino acid can meet nutrient requirements of different stages of the anther culture process; division of anther into a cell cluster is expedited effectively; the cell cluster is differentiated into an embryo; a callus tissue is formed, and differentiated into a plant; and the quantity and the proportion of the inorganic nutrient in the culture solution are appropriate, so that nutrient and physiological requirements of a plant cell in different stages are met sufficiently.

Description

[0001] Technical field [0002] The present invention involves a flower medicine culture solution, which is a plant ionization culture technology. Background technique [0003] The cultivation liquid is like soil. It is a base that depends on the survival and development of the icing material in the organization.The existing flower medicine culture solution cannot meet the nutritional needs of each stage during the cultivation of flower medicine, especially the number and proportion of inorganic nutrients in it are not appropriate, and it is difficult to meet the nutrition and physiological needs of plant cells at all stages. Invention content [0004] The purpose of invention: The technical problem to be solved by the present invention is to provide a cultivation solution for the cultivation of flower medicine to cultivate, which contains a large amount of nutrient elements and trace elements and the proportion of inorganic nutrients.Nutrition needs. [0005] In order to solve ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 谭美兰
Owner NANJING NIANJI REFRIGERATION FOOD
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