Breeding method for gene aggregation related to inosinic acid and intramuscular fat content in high-quality broilers

Abstract

The invention discloses a high-quality broiler chicken inosinic acid and intramuscular fat content related gene polymerization breeding method comprising the following steps: respectively amplifying a gene by using three pairs of primers under the condition of PCR (Polymerase Chain Reaction) by taking two-continuous-generation high-quality broiler chicken genome DNAs (Deoxyribose Nucleic Acids) as templates; judging the sizes of amplification products of the first primer, the second primer and the third primer according to agarose gel electrophoresis, and then, screening the mutation of three alkali bases by using a DNA sequencing technology, wherein the three alkali bases include an exon 9 of an adenylosuccinate lyase gene, a non-coding region of a phosphoribosylglycineamide synthetase-5-aminoimidazole ribonucleotide synthetase-phosphoribosylglycineamide transmethylase gene 5' and an intron 2 of an adipocyte fatty acid binding protein gene; and then, carrying out gene typing and gene frequency analysis on SNPs (Single Nucleotide Polymorphisms) of the three loci by using polyacrylamide gel electrophoresis. By using the high-quality broiler chicken inosinic acid and intramuscular fat content related gene polymerization breeding method, the favorable polymerization of a protogene type can be realized, and meanwhile, the genetic mechanisms of the chicken meat quality, flavor and character can be searched more comprehensively.

Description

technical field [0001] The invention relates to a breeding method, in particular to a breeding method for gene aggregation related to inosinic acid and intramuscular fat content of high-quality broilers. technical background [0002] Improving meat quality and flavor has become an important direction in the research of high-quality broiler breeding, in which inosinic acid and intramuscular fat content are important factors affecting meat quality and flavor traits. However, both inosinic acid and intramuscular fat are affected by many factors, and the progress of conventional breeding is slow; therefore, it is currently more effective to use molecular marker-assisted selection to accelerate genetic progress by using candidate genes related to inosinic acid and intramuscular fat traits. Means, so as to improve chicken quality and flavor breeding at the genetic level. The research on molecular marker screening and marker-assisted selection of muscle quality traits at home and ...

AI Technical Summary

Problems solved by technology

However, the meat quality trait is a comprehensive trait controlled by multiple genes. Molecular assisted breeding only starting from a single gene often cannot achieve the goal of comprehensively improving meat quality and ultimately cultivating high-quality chickens.